Hepatic knockdown of stearoyl-CoA desaturase 1 via RNA interference in obese mice decreases lipid content and changes fatty acid composition

Frontiers in Bioscience

Volumn 12, Issue 10, 2007, Pages 3781-3794

  Xu, Haiyan ^a   Wilcox, Denise ^a   Nguyen, Phong ^a   Voorbach, Martin ^a   Smith, Harriet ^a   Brodjian, Sevan ^a   Suhar, Thomas ^a   Reilly, Regina M ^a   Jacobson, Peer B ^a   Collins, Christine A ^a   Landschulz, Katherine ^a   Surowy, Terry K ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

Ob ob; Obesity; RNA interference; SCD1

Indexed keywords

ADENOVIRIDAE; MUS;

ACYL COENZYME A DESATURASE; FATTY ACID; LIPID;

ANIMAL; ARTICLE; CHEMISTRY; ENZYMOLOGY; GENETICS; LIVER; METABOLISM; MOUSE; OBESITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

ANIMALS; FATTY ACIDS; LIPIDS; LIVER; MICE; OBESITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; STEAROYL-COA DESATURASE;

EID: 34347262079     PISSN: 27686701     EISSN: 27686698     Source Type: Journal    
DOI: 10.2741/2352     Document Type: Article

References (42)

1. Miyazaki, M., W. C. Man & J. M. Ntambi: Targeted disruption of stearoyl-CoA desaturase1 gene in mice causes atrophy of sebaceous and meibomian glands and depletion of wax esters in the eyelid. J Nutr, 131, 2260-8 (2001) (Pubitemid 32851866)
2. Miyazaki, M. & J. M. Ntambi: Role of stearoylcoenzyme A desaturase in lipid metabolism. Prostaglandins Leukot Essent Fatty Acids, 68, 113-21 (2003) (Pubitemid 36216475)
3. Dobrzyn, A. & J. M. Ntambi: The role of stearoyl-CoA desaturase in body weight regulation. Trends Cardiovasc Med, 14, 77-81 (2004) (Pubitemid 38340851)
4. Kaestner, K. H., J. M. Ntambi, T. J. Kelly, Jr. & M. D. Lane: Differentiation-induced gene expression in 3T3-L1 preadipocytes. A second differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem, 264, 14755-61 (1989) (Pubitemid 19237293)
5. Miyazaki, M., A. Dobrzyn, P. M. Elias & J. M. Ntambi: Stearoyl-CoA desaturase-2 gene expression is required for lipid synthesis during early skin and liver development. Proc Natl Acad Sci U S A, 102, 12501-6 (2005) (Pubitemid 41266388)
6. Zheng, Y., S. M. Prouty, A. Harmon, J. P. Sundberg, K. S. Stenn & S. Parimoo: Scd3-a novel gene of the stearoyl-CoA desaturase family with restricted expression in skin. Genomics, 71, 182-91 (2001) (Pubitemid 32165085)
7. Miyazaki, M., M. J. Jacobson, W. C. Man, P. Cohen, E. Asilmaz, J. M. Friedman & J. M. Ntambi: Identification and characterization of murine SCD4, a novel heartspecific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. J Biol Chem, 278, 33904-11 (2003) (Pubitemid 37553224)
8. Zhang, S., Y. Yang & Y. Shi: Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J, 388, 135-42 (2005) (Pubitemid 40732940)
9. Zhang, L., L. Ge, S. Parimoo, K. Stenn & S. M. Prouty: Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J, 340(Pt 1), 255-64 (1999) (Pubitemid 29251114)
10. Beiraghi, S., M. Zhou, C. B. Talmadge, N. Went-Sumegi, J. R. Davis, D. Huang, H. Saal, T. A. Seemayer & J. Sumegi: Identification and characterization of a novel gene disrupted by a pericentric inversion inv (4) (p13.1q21.1) in a family with cleft lip. Gene, 309, 11-21 (2003) (Pubitemid 36513857)
11. Yu, C., Y. Chen, G. W. Cline, D. Zhang, H. Zong, Y. Wang, R. Bergeron, J. K. Kim, S. W. Cushman, G. J. Cooney, B. Atcheson, M. F. White, E. W. Kraegen & G. I. Shulman: Mechanism by which fatty acids inhibit insulin activation of insulin receptor substrate-1 (IRS-1) - associated phosphatidylinositol 3-kinase activity in muscle. J Biol Chem, 277, 50230-6 (2002) (Pubitemid 36042170)
12. Miyazaki, M., S. M. Bruggink & J. M. Ntambi: Identification of mouse palmitoyl-coenzyme A Delta9-desaturase. J Lipid Res, 47, 700-4 (2006)
13. Hu, C. C., K. Qing & Y. Chen: Diet-induced changes in stearoyl-CoA desaturase 1 expression in obesity-prone and-resistant mice. Obes Res, 12, 1264-70 (2004) (Pubitemid 41137470)
14. Miyazaki, M., A. Dobrzyn, W. C. Man, K. Chu, H. Sampath, H. J. Kim & J. M. Ntambi: Stearoyl-CoA desaturase 1 gene expression is necessary for fructosemediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and - independent mechanisms. J Biol Chem, 279, 25164-71 (2004) (Pubitemid 38756766)
15. Legrand, P., D. Catheline, J. M. Hannetel & P. Lemarchal: Stearoyl-CoA desaturase activity in primary culture of chicken hepatocytes. Influence of insulin, glucocorticoid, fatty acids and cordycepin. Int J Biochem, 26, 777-85 (1994)
16. Waters, K. M. & J. M. Ntambi: Insulin and dietary fructose induce stearoyl-CoA desaturase 1 gene expression of diabetic mice. J Biol Chem, 269, 27773-7 (1994) (Pubitemid 24346619)
17. Liang, C. P. & A. R. Tall: Transcriptional profiling reveals global defects in energy metabolism, lipoprotein, and bile acid synthesis and transport with reversal by leptin treatment in ob/ob mouse liver. J Biol Chem, 276, 49066-76 (2001)
18. Lee, K. N., M. W. Pariza & J. M. Ntambi: Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochem Biophys Res Commun, 248, 817-21 (1998) (Pubitemid 28404506)
19. Bene, H., D. Lasky & J. M. Ntambi: Cloning and characterization of the human stearoyl-CoA desaturase gene promoter: transcriptional activation by sterol regulatory element binding protein and repression by polyunsaturated fatty acids and cholesterol. Biochem Biophys Res Commun, 284, 1194-8 (2001) (Pubitemid 32917965)
20. Asilmaz, E., P. Cohen, M. Miyazaki, P. Dobrzyn, K. Ueki, G. Fayzikhodjaeva, A. A. Soukas, C. R. Kahn, J. M. Ntambi, N. D. Socci & J. M. Friedman: Site and mechanism of leptin action in a rodent form of congenital lipodystrophy. J Clin Invest, 113, 414-24 (2004) (Pubitemid 38544185)
21. Kim, H. J., M. Miyazaki & J. M. Ntambi: Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism. J Lipid Res, 43, 1750-7 (2002) (Pubitemid 35168290)
22. Cohen, P., M. Miyazaki, N. D. Socci, A. Hagge-Greenberg, W. Liedtke, A. A. Soukas, R. Sharma, L. C. Hudgins, J. M. Ntambi & J. M. Friedman: Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science, 297, 240-3 (2002) (Pubitemid 34761283)
23. Attie, A. D., R. M. Krauss, M. P. Gray-Keller, A. Brownlie, M. Miyazaki, J. J. Kastelein, A. J. Lusis, A. F. Stalenhoef, J. P. Stoehr, M. R. Hayden & J. M. Ntambi: Relationship between stearoyl-CoA desaturase activity and plasma triglycerides in human and mouse hypertriglyceridemia. J Lipid Res, 43, 1899-907 (2002) (Pubitemid 35315808)
24. Hulver, M. W., J. R. Berggren, M. J. Carper, M. Miyazaki, J. M. Ntambi, E. P. Hoffman, J. P. Thyfault, R. Stevens, G. L. Dohm, J. A. Houmard & D. M. Muoio: Elevated stearoyl-CoA desaturase-1 expression in skeletal muscle contributes to abnormal fatty acid partitioning in obese humans. Cell Metab, 2, 251-61 (2005)
25. Zheng, Y., K. J. Eilertsen, L. Ge, L. Zhang, J. P. Sundberg, S. M. Prouty, K. S. Stenn & S. Parimoo: Scd1 is expressed in sebaceous glands and is disrupted in the asebia mouse. Nat Genet, 23, 268-70 (1999) (Pubitemid 29526420)
26. Miyazaki, M., Y. C. Kim, M. P. Gray-Keller, A. D. Attie & J. M. Ntambi: The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1. J Biol Chem, 275, 30132-8 (2000)
27. Ntambi, J. M., M. Miyazaki, J. P. Stoehr, H. Lan, C. M. Kendziorski, B. S. Yandell, Y. Song, P. Cohen, J. M. Friedman & A. D. Attie: Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci U S A, 99, 11482-6 (2002) (Pubitemid 34920951)
28. Miyazaki, M., H. J. Kim, W. C. Man & J. M. Ntambi: Oleoyl-CoA is the major de novo product of stearoyl-CoA desaturase 1 gene isoform and substrate for the biosynthesis of the Harderian gland 1-alkyl-2, 3-diacylglycerol. J Biol Chem, 276, 39455-61 (2001) (Pubitemid 37371324)
29. Miyazaki, M., Y. C. Kim & J. M. Ntambi: A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis. J Lipid Res, 42, 1018-24 (2001) (Pubitemid 32645781)
30. Dobrzyn, P., A. Dobrzyn, M. Miyazaki, P. Cohen, E. Asilmaz, D. G. Hardie, J. M. Friedman & J. M. Ntambi: Stearoyl-CoA desaturase 1 deficiency increases fatty acid oxidation by activating AMP-activated protein kinase in liver. Proc Natl Acad Sci U S A, 101, 6409-14 (2004) (Pubitemid 38585987)
31. Miyazaki, M., A. Dobrzyn, H. Sampath, S. H. Lee, W. C. Man, K. Chu, J. M. Peters, F. J. Gonzalez & J. M. Ntambi: Reduced adiposity and liver steatosis by stearoyl-CoA desaturase deficiency are independent of peroxisome proliferator-activated receptor-alpha. J Biol Chem, 279, 35017-24 (2004) (Pubitemid 39318138)
32. Jiang, G., Z. Li, F. Liu, K. Ellsworth, Q. Dallas-Yang, M. Wu, J. Ronan, C. Esau, C. Murphy, D. Szalkowski, R. Bergeron, T. Doebber & B. B. Zhang: Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1. J Clin Invest, 115, 1030-8 (2005) (Pubitemid 40489408)
33. Rahman, S. M., A. Dobrzyn, P. Dobrzyn, S. H. Lee, M. Miyazaki & J. M. Ntambi: Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B in muscle. Proc Natl Acad Sci U S A, 100, 11110-5 (2003) (Pubitemid 37140160)
34. Rahman, S. M., A. Dobrzyn, S. H. Lee, P. Dobrzyn, M. Miyazaki & J. M. Ntambi: Stearoyl-CoA desaturase 1 deficiency increases insulin signaling and glycogen accumulation in brown adipose tissue. Am J Physiol Endocrinol Metab, 288, E381-7 (2005) (Pubitemid 40110647)
35. Gutierrez-Juarez, R., A. Pocai, C. Mulas, H. Ono, S. Bhanot, B. P. Monia & L. Rossetti: Critical role of stearoyl-CoA desaturase-1 (SCD1) in the onset of diet-induced hepatic insulin resistance. J Clin Invest, 116, 1686-95 (2006)
36. Dobrzyn, A., P. Dobrzyn, M. Miyazaki, H. Sampath, K. Chu & J. M. Ntambi: Stearoyl-CoA desaturase 1 deficiency increases CTP:choline cytidylyltransferase translocation into the membrane and enhances phosphatidylcholine synthesis in liver. J Biol Chem, 280, 23356-62 (2005) (Pubitemid 40853249)
37. Ntambi, J. M.: Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. J Lipid Res, 40, 1549-58 (1999) (Pubitemid 29418696)
38. Dobrzyn, A. & J. M. Ntambi: The role of stearoyl-CoA desaturase in the control of metabolism. Prostaglandins Leukot Essent Fatty Acids, 73, 35-41 (2005) (Pubitemid 40826926)
39. Neschen, S., K. Morino, L. E. Hammond, D. Zhang, Z. X. Liu, A. J. Romanelli, G. W. Cline, R. L. Pongratz, X. M. Zhang, C. S. Choi, R. A. Coleman & G. I. Shulman: Prevention of hepatic steatosis and hepatic insulin resistance in mitochondrial acyl-CoA:glycerol-sn-3-phosphate acyltransferase 1 knockout mice. Cell Metab, 2, 55-65 (2005) (Pubitemid 43239825)
40. Sun, Y., M. Hao, Y. Luo, C. P. Liang, D. L. Silver, C. Cheng, F. R. Maxfield & A. R. Tall: Stearoyl-CoA desaturase inhibits ATP-binding cassette transporter A1-mediated cholesterol efflux and modulates membrane domain structure. J Biol Chem, 278, 5813-20 (2003) (Pubitemid 36800827)
41. Ricquier, D. & F. Bouillaud: Mitochondrial uncoupling proteins: from mitochondria to the regulation of energy balance. J Physiol, 529 Pt 1, 3-10 (2000)
42. Grav, H. J., K. J. Tronstad, O. A. Gudbrandsen, K. Berge, K. E. Fladmark, T. C. Martinsen, H. Waldum, H. Wergedahl & R. K. Berge: Changed energy state and increased mitochondrial beta-oxidation rate in liver of rats associated with lowered proton electrochemical potential and stimulated uncoupling protein 2 (UCP-2) expression: evidence for peroxisome proliferator-activated receptoralpha independent induction of UCP-2 expression. J Biol Chem, 278, 30525-33 (2003) (Pubitemid 36994556)