Mixed-culture assays for analyzing neuronal synapse formation

Nature Protocols

Volumn 2, Issue 3, 2007, Pages 670-676

  Biederer, Thomas ^a   Scheiffele, Peter ^b  

^a YALE UNIVERSITY   (United States)

^b Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL ADHESION MOLECULE; CELL SURFACE PROTEIN; MEMBRANE PROTEIN; SIGNAL PEPTIDE;

ANIMAL CELL; ARTICLE; ASSAY; CENTRAL NERVOUS SYSTEM; HUMAN; HUMAN CELL; METHODOLOGY; MIXED CELL CULTURE; NERVE CELL; NERVE CELL CULTURE; NONHUMAN; POSTSYNAPTIC MEMBRANE; PRESYNAPTIC MEMBRANE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SYNAPTOGENESIS; TIME; VERTEBRATE; ANIMAL; CHEMISTRY; CULTURE TECHNIQUE; ISOLATION AND PURIFICATION; PROTEOMICS; SYNAPSE;

VERTEBRATA;

ANIMALS; CELL CULTURE TECHNIQUES; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MEMBRANE PROTEINS; NEURONS; PROTEOMICS; SYNAPSES;

EID: 34347225573     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.92     Document Type: Article

References (25)

1. Waites, C.L., Marie Craig, A. & Garner, C.C. Mechanisms of vertebrate synaptogenesis. Annu. Rev. Neurosci. 28, 251-274 (2005).
2. Yamagata, M., Sanes, J.R. & Weiner, J.A. Synaptic adhesion molecules. Curr. Opin. Cell Biol. 15, 621-632 (2003).
3. Scheiffele, P. Cell-cell signaling during synapse formation in the CNS. Annu. Rev. Neurosci. 26, 485-508 (2003).
4. Benson, D.L., Colman, D.R. & Huntley, G.W. Molecules, maps and synapse specificity. Nat. Rev. Neurosci. 2, 899-909 (2001).
5. Hall, A.C., Lucas, F.R. & Salinas, P.C. Axonal remodeling and synaptic differentiation in the cerebellum is regulated by WNT-7a signaling. Cell 100, 525-535 (2000).
6. Umemori, H., Linhoff, M.W., Ornitz, D.M. & Sanes, J.R. FGF22 and its close relatives are presynaptic organizing molecules in the mammalian brain. Cell 118, 257-270 (2004).
7. Scheiffele, P., Fan, J., Choih, J., Fetter, R. & Serafini, T. Neuroligin expressed in nonneuronal cells triggers presynaptic development in contacting axons. Cell 101, 657-669 (2000).
8. Biederer, T. et al. SynCAM, a synaptic adhesion molecule that drives synapse assembly. Science 297, 1525-1531 (2002).
9. Kim, S. et al. NGL family PSD-95-interacting adhesion molecules regulate excitatory synapse formation. Nat. Neurosci. 9, 1294-1301 (2006).
10. Graf, E.R., Zhang, X., Jin, S.-X., Linhoff, M.W. & Craig, A.M. Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins. Cell 119, 1013-1026 (2004).
11. Sara, Y. et al. Selective capability of SynCAM and neuroligin for functional synapse assembly. J. Neurosci. 25, 260-270 (2005).
12. Chih, B., Engelman, H. & Scheiffele, P. Control of excitatory and inhibitory synapse formation by neuroligins. Science 307, 1324-1328 (2005).
13. Prange, O., Wong, T.P., Gerrow, K., Wang, Y.T. & El-Husseini, A. A balance between excitatory and inhibitory synapses is controlled by PSD-95 and neuroligin. Proc. Natl. Acad. Sci. USA 101, 13915-13920 (2004).
14. O'Brien, R.J. et al. Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp. Neuron 23, 309-323 (1999).
15. Fu, Z., Washbourne, P., Ortinski, P. & Vicini, S. Functional excitatory synapses in HEK293 cells expressing neuroligin and glutamate receptors. J. Neurophysiol. 90, 3950-3957 (2003).
16. Nam, C.I. & Chen, L. Postsynaptic assembly induced by neurexin-neuroligin interaction and neurotransmitter. Proc. Natl. Acad. Sci. USA 102, 6137-6142 (2005).
17. Betz, W.J., Mao, F. & Smith, C.B. Imaging exocytosis and endocytosis. Curr. Opin. Neurobiol. 6, 365-371 (1996).
18. Kavalali, E.T., Klingauf, J. & Tsien, R.W. Properties of fast endocytosis at hippocampal synapses. Philos. Trans. R. Soc. London - B 354, 337-346 (1999).
19. Willig, K.I., Rizzoli, S.O., Westphal, V., Jahn, R. & Hell, S.W. STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440, 935-939 (2006).
20. Anderson, T.R., Shah, P.A. & Benson, D.L. Maturation of glutamatergic and GABAergic synapse composition in hippocampal neurons. Neuropharmacology 47, 694-705 (2004).
21. deAnda, F.C. et al. Centrosome localization determines neuronal polarity. Nature 436, 704-708 (2005).
22. Goslin, K., Schreyer, D.J., Skene, J.H. & Banker, G. Development of neuronal polarity: GAP-43 distinguishes axonal from dendritic growth cones. Nature 336, 672-674 (1988).
23. Xu, P. et al. In vitro development of mouse embryonic stem cells lacking JNK/ stress-activated protein kinase-associated protein 1 (JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis. J. Biol. Chem. 278, 48422-48433 (2003).
24. Chih, B., Gollan, L. & Scheiffele, P. Alternative splicing controls selective trans-synaptic interactions of the neuroligin-neurexin complex. Neuron 51, 171-178 (2006).
25. Akins, M.R. & Greer, C.A. Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion. J. Comp. Neurol. 499, 979-989 (2006).