Heparin and EDTA as anticoagulant differentially affect cytokine mRNA level of cultured porcine blood cells

Journal of Immunological Methods

Volumn 324, Issue 1-2, 2007, Pages 38-47

  Duvigneau, J C ^a   Sipos, W ^a   Hartl, R T ^a   Bayer, M ^a   Moldzio, R ^a   Stevenson, L ^b   Adair, B ^b   Gemeiner, M ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Anticoagulant; Cytokine mRNA; EDTA; Heparin; Multiplex; PBMC; Pig; Real time PCR; TaqMan; Whole blood

Indexed keywords

CONCANAVALIN A; CYTOKINE; EDETIC ACID; GAMMA INTERFERON; HEPARIN; INTERLEUKIN 10; INTERLEUKIN 1ALPHA; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 6; LIPOPOLYSACCHARIDE; LIPOPOLYSACCHARIDE BINDING PROTEIN; MESSENGER RNA; RNA;

ANIMAL CELL; ANTICOAGULATION; ARTICLE; BLOOD CELL; BLOOD CLOTTING; CELL CULTURE; CELL ISOLATION; CELL STIMULATION; CONTROLLED STUDY; CYTOKINE RELEASE; IMMUNOSTIMULATION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; T LYMPHOCYTE; TARGET CELL;

ANIMALS; ANTICOAGULANTS; CELLS, CULTURED; CYTOKINES; EDETIC ACID; HEPARIN; LEUKOCYTES, MONONUCLEAR; RNA, MESSENGER; SWINE;

BACTERIA (MICROORGANISMS); SUIDAE; SUS;

EID: 34347214467     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.04.012     Document Type: Article

References (36)

1. Anastase-Ravion S., Blondin C., Cholley B., Haeffner-Cavaillon N., Castellot J.J., and Letourneur D. Heparin inhibits lipopolysaccharide (LPS) binding to leukocytes and LPS-induced cytokine production. J. Biomed. Mater. Res. A 66 (2003) 376
2. Attanasio M., Gori A.M., Giusti B., Pepe G., Comeglio P., Brunelli T., Prisco D., Abbate R., Gensini G.F., and Neri Serneri G.G. Cytokine gene expression in human LPS-and IFNgamma-stimulated mononuclear cells is inhibited by heparin. Thromb. Haemost. 79 (1998) 959
3. Barstad R.M., Ovrum E., Ringdal M.A., Oystese R., Hamers M.J., Veiby O.P., Rolfsen T., Stephens R.W., and Sakariassen K.S. Induction of monocyte tissue factor procoagulant activity during coronary artery bypass surgery is reduced with heparin-coated extracorporeal circuit. Br. J. Haematol. 94 (1996) 517
4. Bosshart H., and Heinzelmann M. Lipopolysaccharide-mediated cell activation without rapid mobilization of cytosolic free calcium. Mol. Immunol. 41 (2004) 1023
5. Brunialti M.K., Kallas E.G., Freudenberg M., Galanos C., and Salomao R. Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood. Cytometry 50 (2002) 14
6. David D., Bani L., Moreau J.L., Demaison C., Sun K., Salvucci O., Nakarai T., de Montalembert M., Chouaib S., Joussemet M., Ritz J., and Theze J. Further analysis of interleukin-2 receptor subunit expression on the different human peripheral blood mononuclear cell subsets. Blood 91 (1998) 165
7. 2+ response amplitude and duration. Nature 386 (1997) 855
8. Duvigneau J.C., Hartl R.T., Teinfalt M., and Gemeiner M. Delay in processing porcine whole blood affects cytokine expression. J. Immunol. Methods 272 (2003) 11
9. Duvigneau J.C., Hartl R.T., Groiss S., and Gemeiner M. Quantitative simultaneous multiplex real-time PCR for the detection of porcine cytokines. J. Immunol. Methods, 306 (2005) 16
10. Eggesbo J.B., Hjermann I., Hostmark A.T., and Kierulf P. LPS induced release of IL-1 beta, IL-6, IL-8 and TNF-alpha in EDTA or heparin anticoagulated whole blood from persons with high or low levels of serum HDL. Cytokine 8 (1996) 152
11. Feske S., Giltnane J., Dolmetsch R., Staudt L.M., and Rao A. Gene regulation mediated by calcium signals in T lymphocytes. Nat.Immunol. 2 (2001) 316
12. Frieri M., and Metcalfe D.D. Analysis of the effect of mast cell granules on lymphocyte blastogenesis in the absence and presence of mitogens: identification of heparin as a granule-associated suppressor factor. J. Immunol. 131 (1983) 1942
13. Goger B., Halden Y., Rek A., Mosl R., Pye D., Gallagher J., and Kungl A.J. Different affinities of glycosaminoglycan oligosaccharides for monomeric and dimeric interleukin-8: a model for chemokine regulation at inflammatory sites. Biochemistry 41 (2002) 1640
14. Hartel C., Bein G., Muller-Steinhardt M., and Kluter H. Ex vivo induction of cytokine mRNA expression in human blood samples. J. Immunol. Methods 249 (2001) 63
15. Hasan M., Najjam S., Gordon M.Y., Gibbs R.V., and Rider C.C. IL-12 is a heparin-binding cytokine. J. Immunol. 162 (1999) 1064
16. Hoffmeister B., Bunde T., Rudawsky I.M., Volk H.D., and Kern F. Detection of antigen-specific T cells by cytokine flow cytometry: the use of whole blood may underestimate frequencies. Eur. J. Immunol. 33 (2003) 3484
17. Hogasen A.K., and Abrahamsen T.G. Heparin suppresses lipopolysaccharide-induced monocyte production of several cytokines, but simultaneously stimulates C3 production. Thromb. Res. 80 (1995) 179
18. Johnell M., Elgue G., Larsson R., Larsson A., Thelin S., and Siegbahn A. Coagulation, fibrinolysis, and cell activation in patients and shed mediastinal blood during coronary artery bypass grafting with a new heparin-coated surface. J. Thorac. Cardiovasc. Surg. 124 (2002) 321
19. Lappegard K.T., Fung M., Bergseth G., Riesenfeld J., and Mollnes T.E. Artificial surface-induced cytokine synthesis: effect of heparin coating and complement inhibition. Ann. Thorac. Surg. 78 (2004) 38
20. Lider O., Mekori Y.A., Miller T., Bar-Tana R., Vlodavsky I., Baharav E., Cohen I.R., and Naparstek Y. Inhibition of T lymphocyte heparanase by heparin prevents T cell migration and T cell-mediated immunity. Eur. J. Immunol. 20 (1990) 493
21. Lipscombe R.J., Nakhoul A.M., Sanderson C.J., and Coombe D.R. Interleukin-5 binds to heparin/heparan sulfate. A model for an interaction with extracellular matrix. J. Leukoc. Biol. 63 (1998) 342
22. Lo C.J., Garcia I., Cryer H.G., and Maier R.V. Calcium and calmodulin regulate lipopolysaccharide-induced alveolar macrophage production of tumor necrosis factor and procoagulant activity. Arch. Surg. 131 (1996) 44
23. Mayringer I., Reindl M., and Berger T. A critical comparison of frequently used methods for the analysis of tumor necrosis factor-alpha expression by human immune cells. J. Immunol. Methods 235 (2000) 33
24. Mummery R.S., and Rider C.C. Characterization of the heparin-binding properties of IL-6. J. Immunol. 165 (2000) 5671
25. Petrovsky N., and Harrison L.C. Cytokine-based human whole blood assay for the detection of antigen-reactive T cells. J. Immunol. Methods 186 (1995) 37
26. Ramsden L., and Rider C.C. Selective and differential binding of interleukin (IL)-1 alpha, IL-1 beta, IL-2 and IL-6 to glycosaminoglycans. Eur. J. Immunol. 22 (1992) 3027
27. Rodenburg R.J., van den Hoogen F.H., van de Putte L.B., and van Venrooij W.J. Peripheral blood monocytes of rheumatoid arthritis patients do not express elevated TNF alpha, IL-1beta, and IL-8 mRNA levels. A comparison of monocyte isolation procedures. J. Immunol. Methods 221 (1998) 169
28. Salek-Ardakani S., Arrand J.R., Shaw D., and Mackett M. Heparin and heparan sulfate bind interleukin-10 and modulate its activity. Blood 96 (2000) 1879
29. Schonherr E., and Hausser H.J. Extracellular matrix and cytokines: a functional unit. Dev. Immunol. 7 (2000) 89
30. Shalekoff S., Page-Shipp L., and Tiemessen C.T. Effects of anticoagulants and temperature on expression of activation markers CD11b and HLA-DR on human leukocytes. Clin. Diagn. Lab. Immunol. 5 (1998) 695
31. Takamatsu H.H., Denyer M.S., Stirling C., Cox S., Aggarwal N., Dash P., Wileman T.E., and Barnett P.V. Porcine gammadelta T cells: possible roles on the innate and adaptive immune responses following virus infection. Vet. Immunol. Immunopathol. 112 (2006) 49
32. Tanaka Y., Kimata K., Adams D.H., and Eto S. Modulation of cytokine function by heparan sulfate proteoglycans: sophisticated models for the regulation of cellular responses to cytokines. Proc. Assoc. Am. Physicians 110 (1998) 118
33. Vanham G.L., Penne G., Vereecken C., Vingerhoets J., and Kestens L. Interleukin-2 receptor subunit expression and function on human peripheral T cells is not dependent on the anticoagulant. Blood 92 (1998) 2989
34. Wang L., Brown J.R., Varki A., and Esko J.D. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L-and P-selectins. J. Clin. Invest 110 (2002) 127
35. Yancy, H., Ayers, S.L., Farrell, D.E., Day, A., & Myers, M.J. (2001) Differential cytokine mRNA expression in swine whole blood and peripheral blood mononuclear cell cultures. Vet. Immunol.Immunopathol.2001.May.10; 79.(1-2):41.-52., 79, 41-52.
36. Zuckermann F.A. Extrathymic CD4/CD8 double positive T cells. Vet. Immunol. Immunopathol., 72 (1999) 55