Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo

Nature Protocols

Volumn 2, Issue 6, 2007, Pages 1479-1489

  Song, Bing ^a   Gu, Yu ^a   Pu, Jin ^a   Reid, Brian ^a   Zhao, Zhiqiang ^a   Zhao, Min ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ACTIVITY; CELL CULTURE; CELL MATURATION; CELL MIGRATION; CLINICAL PROTOCOL; CONTROLLED STUDY; CULTURE MEDIUM; DIRECT CURRENT; ELECTRIC FIELD; EMBRYO; IN VITRO STUDY; IN VIVO STUDY; MICROSCOPY; MODULATION; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; THREE DIMENSIONAL IMAGING; TISSUE CULTURE; WOUND HEALING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CHICK EMBRYO; ELECTRIC STIMULATION; ELECTRICITY; HYDROGEN-ION CONCENTRATION; MICE; RATS; TISSUE CULTURE TECHNIQUES; WOUND HEALING; WOUNDS AND INJURIES;

EID: 34250739896     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.205     Document Type: Article

References (49)

1. DuBois-Reymond, E. Vorläufiger Abriss einer Untersuchung uber den sogenannten Froschstrom und die electomotorischen Fische. Ann. Phy. U. Chem. 58, 1-30 (1843).
2. Chiang, M.C., Cragoe, E.J. Jr. & Variable, J.W. Jr. Intrinsic electric fields promote epithelization of wounds in the newt, Notophthalmus viridescens. Dev. Biol. 146, 377-385 (1991).
3. Barker, A.T., Jaffe, L.F. & Vanable, J.W. Jr. The glabrous epidermis of cavies contains a powerful battery. Am. J. Physiol. 242, R358-R366 (1982).
4. Borgens, R.B., Vanable, J.W. Jr. & Jaffe, L.F. Bioelectricity and regeneration: Large currents leave the stumps of regenerating newt limbs. Proc. Natl. Acad. Sci. USA 74, 4528-4532 (1977).
5. Jaffe, L.F. & Variable, J.W. Jr. Electric fields and wound healing. Clin. Dermatol. 2, 34-44 (1984).
6. Reid, B., Song, B., McCaig, C.D. & Zhao, M. Wound healing in rat cornea: the role of electric currents. FASEB J. 19, 379-386 (2005).
7. Chiang, M., Robinson, K.R. & Vanable, J.W. Jr. ELectrical fields in the vicinity of epithelial wounds in the isolated bovine eye. Exp. Eye Res. 54, 999-1003 (1992).
8. Kloth, L.C. Etectrical stimulation for wound healing: A review of evidence from in vitro studies, animal experiments, and clinical trials. Int. J. Low Extrem. Wounds 4, 23-44 (2005).
9. Nuccitelli, R. Endogenous electric fields in embryos during development, regeneration and wound healing. Radiat. Prot. Dosimetry 106, 375-383 (2003).
10. Nuccitelli, R. A role for endogenous electric fields in wound healing. Curr. Top. Dev. Biol. 58, 1-26 (2003).
11. Robinson, K.R. & Messerli, M.A. Left/right, up/down: The role of endogenous electrical fields as directional signals in development, repair and invasion. Bioessays 25, 759-766 (2003).
12. Sta Iglesia, D.D., Cragoe, E.J. Jr. & Vanable, J.W. Jr. Electric field strength and epithelization in the newt (Notophthalmus viridescens). J. Exp. Zool. 274, 56-62 (1996).
13. Sta Iglesia, D.D. & Vanable, J.W. Jr. Endogenous lateral electric fields around bovine corneal lesions are necessary for and can enhance normal rates of wound healing. Wound Repair Regen. 6, 531-542 (1998).
14. McCaig, C.D., Rajnicek, A.M., Song, B. & Zhao, M. Controlling cell behavior electrically: Current views and future potential. Physiol. Rev. 85, 943-978 (2005).
15. Foulds, I.S. & Barker, A.T. Human skin battery potentials and their possible role in wound heating. Br. J. Dermatol. 109, 515-522 (1983).
16. Hotary, K.B. & Robinson, K.R. Endogenous electrical currents and voltage gradients in Xenopus embryos and the consequences of their disruption. Dev. Biol. 166, 789-800 (1994).
17. Jaffe, L.F. & Stem, C.D. Strong electrical currents leave the primitive streak of chick embryos. Science 206, 569-571 (1979).
18. Shi, R. & Borgens, R.B. Embryonic neuroepithelial sodium transport, the resulting physiological potential, and cranial development. Dev. Biol. 165, 105-116 (1994).
19. Shi, R. & Borgens, R.B. Three-dimensional gradients of voltage during development of the nervous system as invisible coordinates for the establishment of embryonic pattern. Dev. Dyn. 202, 101-114 (1995).
20. +-ATPase and cell membrane potentials comprise a very early step in left-right patterning. Cell 111, 77-89 (2002).
21. Hotary, K.B. & Robinson, K.R. Evidence of a role for endogenous electrical fields in chick embryo development. Development 124 985-996 (1992).
22. Borgens, R.B., Jaffe, L.F. & Cohen, M.J. Large and persistent electrical currents enter the transected lamprey spinal cord. Proc. Natl. Acad. Sci. USA 77, 1209-1213 (1980)
23. Borgens, R.B., Roederer, E. & Cohen, M.J. Enhanced spinat cord regeneration in lamprey by applied electric fields. Science 213 611-617 (1981).
24. Borgens, R.B., Blight, A.R. & McGinnis, M.E. Behavioral recovery induced by applied electric fields after spinal cord hemisection in guinea pig. Science 238, 366-369 (1987).
25. Shapiro, S. et al. Oscillating field stimulation for complete spinal cord injury in humans: A phase 1 trial. J. Neurosurg. Spine 2, 3-10 (2005).
26. McCaig, C.D. & Zhao, M. Physiological electrical fields modify cell behaviour. Bioessays 19, 819-826 (1997).
27. Mycielska, M.E. & Djamgoz, M.B. Cellular mechanisms of direct-current electric field effects: Galvanotaxis and metastatic disease. J. Cell Sci. 117, 1631-1639 (2004).
28. Erickson, C.A. & Nuccitelli, R. Embryonic fibroblast motility and orientation can be influenced by physiological electric fields. J. Cell Biol. 98, 296-307 (1984).
29. Hinkle, L., McCaig, C.D. & Robinson, K.R. The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field. J. Physiol. 314, 121-135 (1981).
30. McCaig, C.D., Rajnicek, A.M., Song, B. & Zhao, M. Has etectrical growth cone guidance found its potential? Trends Neurosci. 25, 354-359 (2002).
31. Nishimura, K.Y., Isseroff, R.R. & Nuccitelli, R. Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mammalian wounds. J. Cell Sci. 109, 199-207 (1996).
32. Pullar, C.E. et al. β4 Integrin and EGF coordinately regulate electric field-mediated directional migration via Rac1. Mol. Biol. Cell 17, 4925-4935 (2006).
33. Song, B., Zhao, M., Forrester, J.V. & McCaig, C.D. Electrical cues regulate the orientation and frequency of cell division and the rate of wound healing in vivo. Proc. Natl. Acad. 5ci. USA 99, 13577-13582 (2002).
34. Zhao, M., Forrester, J.V. & McCaig, C.D. A small, physiological electric field orients cell division. Proc. Natl. Acad. Sci. USA 96, 4942-4946 (1999).
35. Zhao, M. et al. Electrical signals control wound healing through phosphatidylinositol-3-OH kinase-gamma and PTEN. Nature 442, 457-460 (2006).
36. Vanhaesebroeck, B. Charging the batteries to heal wounds through PI3K. Nat. Chem. Biol. 2, 453-455 (2006).
37. Borgens, R.B., Shi, R., Mohr, T.J. & Jaeger, C.B. Mammalian cortical astrocytes align themselves in a physiological voltage gradient. Exp. Neurol. 128, 41-49 (1994).
38. Cho, M.R., Thatte, H.S., Lee, R.C. & Golan, D.E. Induced redistribution of cell surface receptors by alternating current electric fields. FASEB J. 8, 771-776 (1994).
39. Cooper, M.S. & Keller, R.E. Perpendicular orientation and directional migration of amphibian neural crest cells in dc electrical fields. Proc. Natl. Acad. Sci. USA 81, 160-164 (1984).
40. Jaffe, L.F. Electrophoresis along cell membranes. Nature 265 600-602 (1977).
41. Patel, N. & Poo, M.M. Orientation of neurite growth by extracellular electric fields. J. Neurosci. 2, 483-496 (1982).
42. Poo, M.M. & Robinson, K.R. Electrophoresis of concanavalin A receptors along embryonic muscle cell membrane. Nature 265, 602-605 (1977).
43. Poo, M.M., Poo, W.J. & Lam, J.W. Lateral electrophoresis and diffusion of concanavalin A receptors in the membrane of embryonic muscle cell. J. Cell Biol. 76, 483-501 (1978).
44. Zhao, M., Agius-Fernandez, A., Forrester, J.V. & McCaig, C.D. Orientation and directed migration of cultured corneal epithelial cells in small electric fields are serum dependent. J. Cell Sci. 109 (Pt 6): 1405-1414 (1996).
45. Robinson, K.R. Endogenous and applied electrical currents: Their measurement and application. in Electric Fields in Vertebrate Repair (ed. Alan R. Liss) 1-25 (John Wiley & Sons, 1989).
46. Zhao, M., Jin, T., McCaig, C.D., Forrester, J.V. & Devreotes, P.N. Genetic analysis of the role of G protein-coupled receptor signaling in etectrotaxis. J. Cell Biol. 157, 921-927 (2002).
47. Eagle, H. Buffer combinations for mammalian cell culture. Science 174, 500-503 (1971).
48. Jenkins, L.S., Duerstock, B.S. & Borgens, R.B. Reduction of the current of injury leaving the amputation inhibits limb regeneration in the red spotted newt. Dev. Biol. 178, 251-262 (1996).
49. Adams, D. & Levin, M. Strategies and techniques for investigation of biophysical signals in patterning. in Analysis of Growth Factor Signaling in Embryos (eds. Whitman, M. & Sater, A.K.) 177-214 (Methods in Signal Transduction Series, CRC Press, 2006).