The metabolic role of isoleucine in detoxification of ammonia in cultured mouse neurons and astrocytes

Neurochemistry International

Volumn 50, Issue 7-8, 2007, Pages 1042-1051

  Johansen, Maja L ^a   Bak, Lasse K ^a   Schousboe, Arne ^a   Iversen, Peter ^b   Sørensen, Michael ^b   Keiding, Susanne ^b   Vilstrup, Hendrik ^b   Gjedde, Albert ^b   Ott, Peter ^b   Waagepetersen, Helle S ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Aspartate; Energy; Glucose; Glutamate; Glutamine; Hepatic encephalopathy; Metabolism; TCA cycle

Indexed keywords

ALANINE; AMMONIA; AMMONIUM CHLORIDE; ASPARTIC ACID; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; ISOLEUCINE; TRICARBOXYLIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; CATABOLISM; CELL CULTURE; CONTROLLED STUDY; DETOXIFICATION; GLUCOSE METABOLISM; GLYCOLYSIS; MASS SPECTROMETRY; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; SYNTHESIS;

AMMONIA; ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; CARBON ISOTOPES; CELLS, CULTURED; CITRIC ACID CYCLE; GLUCOSE; ISOLEUCINE; ISOTOPE LABELING; METABOLIC DETOXICATION, DRUG; MICE; MICE, INBRED STRAINS; MODELS, BIOLOGICAL; NEURONS;

EID: 34250718524     PISSN: 01970186     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neuint.2007.01.009     Document Type: Article

References (33)

1. Albrecht J., and Dolinska M. Glutamine as a pathogenic factor in hepatic encephalopathy. J. Neurosci. Res. 65 (2001) 1-5
2. Bak L.K., Schousboe A., Sonnewald U., and Waagepetersen H.S. Glucose is necessary to maintain neurotransmitter homeostasis during synaptic activity in cultured glutamatergic neurons. J. Cereb. Blood Flow Metab. 26 (2006) 1285-1297
3. Biemann K. The mass spectra of isotopically labeled molecules, in Mass spectrometry; Organic chemical applications (1962), McGraw-Hill, New York pp. 223-227
4. Clarke D.D., and Sokoloff L. Circulation and energy metabolism of the brain. In: Siegel G.J., Agranoff B.W., Fisher S.K., Albers R.W., and Uhler M.D. (Eds). Basic Neurochemistry (1999), Lippincott-Raven Publishers, Philadelphia, USA 637-669
5. Drejer J., Larsson O.M., Kvamme E., Svenneby G., Hertz L., and Schousboe A. Ontogenetic development of glutamate metabolizing enzymes in cultured cerebellar granule cells and in cerebellum in vivo. Neurochem. Res. 10 (1985) 49-62
6. Drejer J., and Schousboe A. Selection of a pure cerebellar granule cell culture by kainate treatment. Neurochem. Res. 14 (1989) 751-754
7. Fitzpatrick S.M., Hetherington H.P., Behar K.L., and Shulman R.G. The flux from glucose to glutamate in the rat brain in vivo as determined by 1H-observed, 13C-edited NMR spectroscopy. J. Cereb. Blood Flow Metab. 10 (1990) 170-179
8. Geddes J.W., and Wood J.D. Changes in the amino acid content of nerve endings (synaptosomes) induced by drugs that alter the metabolism of glutamate and gamma-aminobutyric acid. J. Neurochem. 42 (1984) 16-24
9. Hawkins R.A., Peterson D.R., and Vina J.R. The complementary membranes forming the blood-brain barrier. IUBMB. Life 54 (2002) 101-107
10. Hertz L., Juurlink B.H.J., Fosmark H., and Schousboe A. Astrocytes in primary cultures. In: Pfeiffer S.E. (Ed). Neuroscience Approached through Cell Culture (1982), CRC Press, Boca Raton 175-186
11. Hertz L., Murthy C.R., Lai J.C., Fitzpatrick S.M., and Cooper A.J. Some metabolic effects of ammonia on astrocytes and neurons in primary cultures. Neurochem. Pathol. 6 (1987) 97-129
12. Hertz L., Juurlink B.H.J., Hertz E., Fosmark H., and Schousboe A. Preparation of primary cultures of mouse (rat) astrocytes. In: Shahar A., de Vellis J., Vernadakis A., and Haber B. (Eds). A Dissection and Tissue Culture Manual for the Nervous System (1989), Liss, New York 105-108
13. Hindfelt B., Plum F., and Duffy T.E. Effect of acute ammonia intoxication on cerebral metabolism in rats with portacaval shunts. J. Clin. Invest. 59 (1977) 386-396
14. Juurlink B.H., and Hertz L. Plasticity of astrocytes in primary cultures: an experimental tool and a reason for methodological caution. Dev. Neurosci. 7 (1985) 263-277
15. Kala G., and Hertz L. Ammonia effects on pyruvate/lactate production in astrocytes-interaction with glutamate. Neurochem. Int. 47 (2005) 4-12
16. Lai J.C., and Cooper A.J. Brain alpha-ketoglutarate dehydrogenase complex: kinetic properties, regional distribution, and effects of inhibitors. J. Neurochem. 47 (1986) 1376-1386
17. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
18. Mason G.F., Rothman D.L., Behar K.L., and Shulman R.G. NMR determination of the TCA cycle rate and alpha-ketoglutarate/glutamate exchange rate in rat brain. J. Cereb. Blood Flow Metab. 12 (1992) 434-447
19. McKhann G.M., and Tower D.B. Ammonia toxicity and cerebral oxidative metabolism. Am. J. Physiol. 200 (1961) 420-424
20. Norenberg M.D., and Martinez-Hernandez A. Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res. 161 (1979) 303-310
21. Ott P., Clemmesen O., and Larsen F.S. Cerebral metabolic disturbances in the brain during acute liver failure: from hyperammonemia to energy failure and proteolysis. Neurochem. Int. 47 (2005) 13-18
22. Schousboe A., Meier E., Drejer J., and Hertz L. Preparation of primary cultures of mouse (rat) cerebellar granule cells. In: Shahar A., de Vellis J., Vernadakis A., and Haber B. (Eds). A Dissection and Tissue Culture Manual for the Nervous System (1989), Liss, New York 183-186
23. Schousboe A., Westergaard N., Sonnewald U., Petersen S.B., Huang R., Peng L., and Hertz L. Glutamate and glutamine metabolism and compartmentation in astrocytes. Dev. Neurosci. 15 (1993) 359-366
24. Sickmann H.M., Schousboe A., Fosgerau K., and Waagepetersen H.S. Compartmentation of lactate originating from glycogen and glucose in cultured astrocytes. Neurochem. Res. 30 (2005) 1295-1304
25. Sonnewald U., Kortner T.M., Qu H., Olstad E., Sunol C., Bak L.K., Schousboe A., and Waagepetersen H.S. Demonstration of extensive GABA synthesis in the small population of GAD positive neurons in cerebellar cultures by the use of pharmacological tools. Neurochem. Int. 48 (2006) 572-578
26. Sonnewald U., Olstad E., Qu H., Babot Z., Cristofol R., Sunol C., Schousboe A., and Waagepetersen H. First direct demonstration of extensive GABA synthesis in mouse cerebellar neuronal cultures. J. Neurochem. 91 (2004) 796-803
27. Waagepetersen H.S., Hansen G.H., Fenger K., Lindsay J.G., Gibson G., and Schousboe A. Cellular mitochondrial heterogeneity in cultured astrocytes as demonstrated by immunogold labeling of alpha-ketoglutarate dehydrogenase. Glia 53 (2006) 225-231
28. Waagepetersen H.S., Sonnewald U., Larsson O.M., and Schousboe A. A possible role of alanine for ammonia transfer between astrocytes and glutamatergic neurons. J. Neurochem. 75 (2000) 471-479
29. Waagepetersen H.S., Sonnewald U., Larsson O.M., and Schousboe A. Multiple compartments with different metabolic characteristics are involved in biosynthesis of intracellular and released glutamine and citrate in astrocytes. Glia 35 (2001) 246-252
30. Yudkoff M. Disorders of amino acid metabolism. In: Siegel G.J., Albers R.W., Brady S.T., and Price D.L. (Eds). Basic Neurochemistry. 7th ed. (2006), Elsevier, Amsterdam 667-683
31. 15N. Neurochem. Res. 15 (1990) 1191-1196
32. Zaganas I., Waagepetersen H.S., Georgopoulos P., Sonnewald U., Plaitakis A., and Schousboe A. Differential expression of glutamate dehydrogenase in cultured neurons and astrocytes from mouse cerebellum and cerebral cortex. J. Neurosci. Res. 66 (2001) 909-913
33. Zwingmann C., Chatauret N., Leibfritz D., and Butterworth R.F. Selective increase of brain lactate synthesis in experimental acute liver failure: results of a [H{single bond}C] nuclear magnetic resonance study. Hepatology 37 (2003) 420-428