A possible role of alanine for ammonia transfer between astrocytes and glutamatergic neurons

Journal of Neurochemistry

Volumn 75, Issue 2, 2000, Pages 471-479

  Waagepetersen, Helle S ^a   Sonnewald, Ursula ^b   Larsson, Orla M ^a   Schousboe, Arne ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

Gas chromatography mass spectrometry; Glucose; Magnetic resonance spectroscopy

Indexed keywords

ALANINE; AMMONIA; GLUTAMIC ACID; GLUTAMINE; LACTIC ACID; NITROGEN;

AMINO ACID METABOLISM; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ASTROCYTE; CELL METABOLISM; CELL TRANSPORT; GRANULE CELL; MOUSE; NERVE CELL; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; TRANSAMINATION;

ANIMALIA;

EID: 0033929168     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2000.0750471.x     Document Type: Article

References (42)

1. Berl S. and Clarke D. D. (1983) The metabolic compartmentation concept, in Glutamine, Glutamate and GABA in the Central Nervous System (Hertz L., Kvamme E., McGeer E. G., and Schousboe A., eds), pp. 205-217. Liss, New York.
2. Biemann K. (1962) Mass spectrometry, in Organic Chemistry Applications, pp. 223-227. McGraw, New York.
3. Bittar P. G., Charnay Y., Pellerin L., Bouras C., and Magistretti P. J. (1996) Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain. J. Cereb. Blood Flow Metab. 16, 1079-1089.
4. Chee P. Y., Dahl J. L., and Fahien L. A. (1979) The purification and properties of rat brain glutamate dehydrogenase. J. Neurochem. 33, 53-60.
5. Colon A. D., Plaitakis A., Perakis A., Berl S., and Clarke D. D. (1986) Purification and characterization of a soluble and a particulate glutamate dehydrogenase from rat brain. J. Neurochem. 46, 1811-1819.
6. Drejer J. and Schousboe A. (1989) Selection of a pure cerebellar granule cell culture by kainate treatment. Neurochem. Res. 14, 751-754.
7. Dringen R., Wiesinger H., and Hamprecht B. (1993) Uptake of L-lactate by cultured rat brain neurons. Neurosci. Lett. 163, 5-7.
8. Dringen R., Peters H., Wiesinger H., and Hamprecht B. (1995) Lactate transport by cultured glial cells. Dev. Neurosci. 17, 63-69.
9. Erecińska M., Nelson D., Nissim I., Daikhin Y., and Yudkoff M. (1994) Cerebral alanine transport and alanine aminotransferase reaction: alanine as a source of neuronal glutamate. J. Neurochem. 62, 1953-1964.
10. Geddes J. W. and Wood J. D. (1984) Changes in the amino acid content of nerve endings (synaptosomes) induced by drugs that alter the metabolism of glutamate and γ-aminobutyric acid. J. Neurochem. 42, 16-24.
11. Gegelashvili G. and Schousboe A. (1998) Cellular distribution and kinetic properties of high-affinity glutamate transporters. Brain Res. Bull. 45, 233-238.
12. Gegelashvili G., Civenni G., Racagni G., Danbolt N. C., Schousboe I., and Schousboe A. (1996) Glutamate receptor agonists up-regulate glutamate transporter GLAST in astrocytes. Neuroreport 8, 261-265.
13. Hertz L., Juurlink B. H. J., Fosmark H., and Schousboe A. (1982) Astrocytes in primary cultures, in Neuroscience Approached Through Cell Culture. Vol. 1 (Pfeiffer S. E., ed), pp. 175-186. CRC Press, Boca Raton.
14. Hertz L., Juurlink B. H. J., Hertz E., Fosmark H., and Schousboe A. (1989) Preparation of primary cultures of mouse (rat) astrocytes, in A Dissection and Tissue Culture Manual for the Nervous System (Shahar A., de Vellis J., Vernadakis A., and Haber B., eds), pp. 105-108. Liss, New York.
15. Izumi Y., Benz A. M., Zorumski C. F., and Olney J. W. (1994) Effects of lactate and pyruvate on glucose deprivation in rat hippocampal slices. Neuroreport 5, 617-620.
16. Kunst A., Draeger B., and Ziegenhorn J. (1984) D-Glucose: UV methods with hexokinase and glucose-6-phosphate dehydrogenase, in Methods of Enzymatic Analysis (Bergmeyer H. U., ed), pp. 163-172. Verlag Chemie, Weinheim.
17. Laake J. H., Takumi Y., Eidet J., Torgner I. A., Roberg B., Kvamme E., and Ottersen O. P. (1999) Postembedding immunogold labelling reveals subcellular localization and pathway-specific enrichment of phosphate activated glutaminase in rat cerebellum. Neuroscience 88, 1137-1151.
18. Lowry O. H., Rosebrough N. J., Farr A. L., and Randall R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
19. Mawhinney T. P., Robinett R. S. R., Atalay A., and Madson M. A. (1986) Analysis of amino acids as their tert-butyldimethylsilyl derivatives by gas-liquid chromatography and mass spectrometry. J. Chromatogr. 358, 231-242.
20. Noll F. (1984) L-(+)-Lactate, in Methods of Enzymatic Analysis (Bergmeyer H. U., ed), pp. 582-588. Verlag Chemie, Weinheim.
21. Norenberg M. D. and Martinez-Hernandez A. (1979) Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res. 161, 303-310.
22. Pellerin L. and Magistretti P. J. (1994) Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization. Proc. Natl. Acad. Sci. USA 91, 10625-10629.
23. Pellerin L., Pellegri G., Bittar P. G., Charnay Y., Bouras C., Martin J. L., Stella N., and Magistretti P. J. (1998) Evidence supporting the existence of an activity-dependent astrocyte-neuron lactate shuttle. Dev. Neurosci. 20, 291-299.
24. Peng L., Schousboe A., and Hertz L. (1991) Utilization of α-ketoglutarate as a precursor for transmitter glutamate in cultured cerebellar granule cells. Neurochem. Res. 16, 29-34.
25. Schousboe A., Meier E., Drejer J., and Hertz L. (1989) Preparation of primary cultures of mouse (rat) cerebellar granule cells, in A Dissection and Tissue Culture Manual for the Nervous System (Shahar A., de Vellis J., Vernadakis A., and Haber B., eds), pp. 183-186. Liss, New York.
26. Schousboe A., Westergaard N., Waagepetersen H. S., Larsson O. M., Bakken I. J., and Sonnewald U. (1997) Trafficking between glia and neurons of TCA-cycle intermediates and related metabolites. Glia 21, 99-105.
27. Schurr A., West C. A., and Rigor B. M. (1988) Lactate supported synaptic function in the rat hippocampal slice preparation. Science 240, 1326-1328.
28. Schurr A., Payne R. S., Miller J. J., and Rigor B. M. (1997) Brain lactate is an obligatory aerobic energy substrate for functional recovery after hypoxia: further in vitro validation. J. Neurochem. 69, 423-426.
29. Sokoloff L. (1992) The brain as a chemical machine. Prog. Brain Res. 94, 19-33.
30. 13C]NMR spectroscopy of cultured neurons and astrocytes. Neurosci. Lett. 128, 235-239.
31. Tsacopoulos M. and Magistretti P. J. (1996) Metabolic coupling between glia and neurons. J. Neurosci. 16, 877-885.
32. Vannucci S. J., Maher F., and Simpson I. A. (1997) Glucose transporter proteins in brain: delivery of glucose to neurons and glia. Glia 21, 2-21.
33. 13C-NMR spectroscopy. J. Cereb. Blood Flow Metab. 18, 109-117.
34. 13C NMR spectroscopy. Dev. Neurosci. 20, 310-320.
35. Walz W. and Mukerji S. (1988) Lactate release from cultured astrocytes and neurons: a comparison. Glia 1, 366-370.
36. Westergaard N., Fosmark H., and Schousboe A. (1991) Metabolism and release of glutamate in cerebellar granule cells cocultured with astrocytes from cerebellum or cerebral cortex. J. Neurochem. 56, 59-66.
37. Westergaard N., Vanning T., Peng L., Sonnewald U., Hertz L., and Schousboe A. (1993) Uptake, release and metabolism of alanine in neurons and astrocytes in primary cultures. J. Neurosci. Res. 35, 540-545.
38. Westergaard N., Sonnewald U., and Schousboe A. (1995) Metabolic trafficking between neurons and astrocytes: the glutamate/glutamine cycle revisited. Dev. Neurosci. 17, 203-211.
39. Yu A. C. H., Hertz E., and Hertz L. (1984) Alterations in uptake and release rates for GABA, glutamate, and glutamine during biochemical maturation of highly purified cultures of cerebral cortical neurons, a GABAergic preparation. J. Neurochem. 42, 951-960.
40. 15N]Aspartate metabolism in cultured astrocytes. Studies with gas chromatography-mass spectrometry. Biochem. J. 241, 193-201.
41. Yudkoff M., Nissim I., Nelson D., Lin Z.-P., and Erecińska M. (1991) Glutamate dehydrogenase reaction as a source of glutamic acid in synaptosomes. J. Neurochem. 57, 153-160.
42. Yudkoff M., Nissim I., Hertz L., Pleasure D., and Erecińska M. (1992) Nitrogen metabolism: neuronal-astroglial relationships. Prog. Brain Res. 94, 213-224.