Production, purification and functional validation of human secreted amyloid precursor proteins for use as neuropharmacological reagents

Journal of Neuroscience Methods

Volumn 164, Issue 1, 2007, Pages 68-74

  Turner, Paul R ^a   Bourne, Katie ^a   Garama, Daniel ^a   Carne, Alan ^a   Abraham, Wickliffe C ^a   Tate, Warren P ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Alzheimer's disease; Amyloid precursor protein; Neuropharmacological reagent; One step purification; SAPP ; SAPP ; Secreted fragment

Indexed keywords

AMYLOID PRECURSOR PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LUCIFERASE;

ALZHEIMER DISEASE; ANTIBODY SPECIFICITY; ARTICLE; CELL STRAIN COS7; CELL VIABILITY; CONTROLLED STUDY; EMBRYO; FAST PROTEIN LIQUID CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; NEUROBLASTOMA; NEUROPHARMACOLOGY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; SILVER STAINING; VALIDATION STUDY;

ALZHEIMER DISEASE; AMYLOID BETA-PROTEIN PRECURSOR; ANIMALS; ANTIBODY SPECIFICITY; CELL CULTURE TECHNIQUES; CELL LINE; CELL LINE, TUMOR; CELL SURVIVAL; CERCOPITHECUS AETHIOPS; CHROMATOGRAPHY, LIQUID; COS CELLS; HUMANS; IMMUNOBLOTTING; NEUROCHEMISTRY; NEUROPROTECTIVE AGENTS; PROTEIN STRUCTURE, TERTIARY; SILVER STAINING; TRANS-ACTIVATION (GENETICS);

EID: 34250626037     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.04.001     Document Type: Article

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