Purification of the M flax-rust resistance protein expressed in Pichia pastoris

Plant Journal

Volumn 50, Issue 6, 2007, Pages 1107-1117

  Schmidt, Simon A ^a,d   Williams, Simon J ^a   Wang, Ching I A ^b   Sornaraj, Pradeep ^a   James, Ben ^a   Kobe, Bostjan ^b   Dodds, Peter N ^c   Ellis, Jeffrey G ^c   Anderson, Peter A ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c CSIRO   (Australia)

^d AUSTRALIAN WINE RESEARCH INSTITUTE   (Australia)

Author keywords

Disease resistance; Flax; Pichia pastoris; Recombinant protein; TIR NBS LRR

Indexed keywords

DISEASES; GENETIC ENGINEERING; PLANTS (BOTANY);

DISEASE RESISTANCE; RECOMBINANT PROTEIN;

PROTEINS;

RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; BASIDIOMYCETES; BIOSYNTHESIS; FLAX; GENE; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PICHIA; PLANT DISEASE; PLASMID;

BASIDIOMYCOTA; FLAX; GENE EXPRESSION; GENES, PLANT; GENETIC VECTORS; PICHIA; PLANT DISEASES; PLANT PROTEINS; PLASMIDS; RECOMBINANT PROTEINS;

DISEASES; GENETIC ENGINEERING; PLANTS; PROTEINS;

FUNGI; LINUM USITATISSIMUM; MELAMPSORA LINI; PICHIA PASTORIS;

EID: 34250159521     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2007.03104.x     Document Type: Article

References (43)

1. Ackers, G.K. (1976) Molecular sieve methods of analysis. In The Proteins, Vol. 1 (Neurath, H. Hill, R.L. eds). New York : Academic press, pp. 1 94.
2. Al-Daoude, A., Zabala, M.D., Ko, J.H. Grant, M. (2005) RIN13 is a positive regulator of the plant disease resistance protein RPM1. Plant Cell, 17, 1016 28.
3. Anderson, P.A., Lawrence, G.J., Morrish, B.C., Ayliffe, M.A., Finnegan, E.J. Ellis, J.G. (1997) Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region. Plant Cell, 9, 641 51.
4. Belkhadir, Y., Nimchuk, Z., Hubert, D.A., Mackey, D. Dangl, J.L. (2004) Arabidopsis RIN4 negatively regulates disease resistance mediated by RPS2 and RPM1 downstream or independent of the NDR1 signal modulator and is not required for the virulence functions of bacterial Type III effectors AvrRpt2 or AvrRpm1. Plant Cell, 16, 2822 35.
5. Bendahmane, A., Farnham, G., Moffett, P. Baulcombe, D.C. (2002) Constitutive gain-of-function mutants in a nucleotide binding site-leucine rich repeat protein encoded at the Rx locus of potato. Plant J. 32, 195 204.
6. van der Biezen, E.A., Freddie, C.T., Kahn, K., Parker, J.E. Jones, J.D.G. (2002) Arabidopsis RPP4 is a member of the RPP5 multigene family of TIR-NB-LRR genes and confers downy mildew resistance through multiple signalling components. Plant J. 29, 439 51.
7. Brake, A.J. (1989) Secretion of heterologous proteins directed by the yeast alpha-factor leader. Biotechnology, 13, 269 80.
8. Brake, A.J., Merryweather, J.P., Coit, D.G., Heberlein, U.A., Masiarz, F.R., Mullenbach, G.T., Urdea, M.S., Valenzuela, P. Barr, P.J. (1984) Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA, 81, 4642 6.
9. Catanzariti, A.M., Dodds, P.N., Lawrence, G.J., Ayliffe, M.A. Ellis, J.G. (2006) Haustorially expressed secreted proteins from flax rust are highly enriched for avirulence elicitors. Plant Cell, 18, 243 56.
10. Chen, W.Y., Wu, C.F. Liu, C.C. (1996) Interactions of imidazole and proteins with immobilized Cu(II) ions: effects of structure, salt concentration, and pH in affinity and binding capacity. J. Colloid Interface Sci. 180, 135 43.
11. Dinesh-Kumar, S.P., Tham, W.H. Baker, B.J. (2000) Structure-function analysis of the tobacco mosaic virus resistance gene N. Proc. Natl Acad. Sci. USA, 97, 14789 94.
12. Dodds, P.N., Lawrence, G.J. Ellis, J.G. (2001) Six amino acid changes confined to the leucine-rich repeat beta-strand/beta-turn motif determine the difference between the P and P2 rust resistance specificities in flax. Plant Cell, 13, 163 78.
13. Dodds, P.N., Lawrence, G.J., Catanzariti, A.M., Ayliffe, M.A. Ellis, J.G. (2004) The Melampsora lini AvrL567 avirulence genes are expressed in haustoria and their products are recognized inside plant cells. Plant Cell, 16, 755 68.
14. Dodds, P.N., Lawrence, G.J., Catanzariti, A.M., Teh, T., Wang, C.I.A., Ayliffe, M.A., Kobe, B. Ellis, J.G. (2006) Direct protein interaction underlies gene-for-gene specificity and coevolution of the flax resistance genes and flax rust avirulence genes. Proc. Natl Acad. Sci. USA, 103, 8888 93.
15. Ellis, J.G., Lawrence, G.J., Finnegan, E.J. Anderson, P.A. (1995) Contrasting complexity of two rust resistance loci in flax. Proc. Natl. Acad. Sci. U. S. A. 92, 4185 8.
16. Ellis, J., Dodds, P. Pryor, T. (2000) Structure, function and evolution of plant disease resistance genes. Curr. Opin. Plant Biol. 3, 278 84.
17. Flor, H.H. (1956) The complementary genic systems in flax and flax rust. Adv. Genet. 8, 29 54.
18. Fuller, R.S., Brake, A.J. Thorner, J. (1989) Intracellular targeting and structural conservation of a prohormone-processing endoprotease. Science, 246, 482 6.
19. Gatlin, C.L., Kleemann, G.R., Hays, L.G., Link, A.J. Yates, J.R. (1998) Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography-microspray and nanospray mass spectrometry. Anal. Biochem. 263, 93 101.
20. Islam, M.R. Shepherd, K.W. (1991) Present status of genetics of rust resistance in flax. Euphytica, 55, 255 67.
21. Jia, Y., McAdams, S.A., Bryan, G.T., Hershey, H.P. Valent, B. (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. EMBO J. 19, 4004 14.
22. Kruijt, M., De Kock, M.J.D. De Wit, P. (2005) Receptor-like proteins involved in plant disease resistance. Mol. Plant Pathol. 6, 85 97.
23. Lawrence, G.J., Ellis, J.G., Finnegan, E.J., Dennis, E.S. Peacock, W.J. (1989) Tagging rust resistance genes in flax. In Breeding Research: the Key To the Survival of the Earth (Iyama, S. Takeda, G. eds). Tsukuba, Japan : Organising Committee of the Sixth International Congress SABRAO, pp. 535 8.
24. Leister, R.T. Katagiri, F. (2000) A resistance gene product of the nucleotide binding site - leucine rich repeats class can form a complex with bacterial avirulence proteins in vivo. Plant J. 22, 345 54.
25. Luck, J.E., Lawrence, G.J., Finnegan, E.J., Jones, D.A. Ellis, J.G. (1998) A flax transposon identified in two spontaneous mutant alleles of the L6 rust resistance gene. Plant J. 16, 365 9.
26. Martin, G.B. (1999) Functional analysis of plant disease resistance genes and their downstream effectors. Curr. Opin. Plant Biol. 2, 273 9.
27. Mestre, P. Baulcombe, D.C. (2006) Elicitor-mediated oligomerization of the tobacco N disease resistance protein. Plant Cell, 18, 491 501.
28. Meyers, B.C., Dickerman, A.W., Michelmore, R.W., Sivaramakrishnan, S., Sobral, B.W. Young, N.D. (1999) Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J. 20, 317 32.
29. Meyers, B.C., Kozik, A., Griego, A., Kuang, H.H. Michelmore, R.W. (2003) Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell, 15, 809 34.
30. Moffett, P., Farnham, G., Peart, J. Baulcombe, D.C. (2002) Interaction between domains of a plant NBS-LRR protein in disease resistance-related cell death. EMBO J. 21, 4511 9.
31. Nielsen, H., Engelbrecht, J., Brunak, S. vonHeijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1 6.
32. Sharma, S. Agarwal, G.P. (2001) Interactions of proteins with immobilized metal ions - Role of ionic strength and pH. J. Colloid Interface Sci. 243, 61 72.
33. Sreerama, N. Woody, R.W. (2000) Estimation of protein secondary structure from circular dichroism spectra: comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Anal. Biochem. 287, 252 60.
34. Sreerama, N. Woody, R.W. (2004) Computation and analysis of protein circular dichroism spectra. Method Enzymol. 383, 318 51.
35. Staskawicz, B.J., Ausubel, F.M., Baker, B.J., Ellis, J.G. Jones, J.D.G. (1995) Molecular genetics of plant disease resistance. Science, 268, 661 7.
36. Stratton, J., Chiruvolu, V. Meagher, M. (1998) High cell-density fermentation. In Pichia Protocols (Higgins, D.R. Cregg, J.M. eds). Totowa, New Jersey : Humana Press, pp. 107 20.
37. Tameling, W.I.L., Elzinga, S.D.J., Darmin, P.S., Vossen, J.H., Takken, F.L.W., Haring, M.A. Cornelissen, B.J.C. (2002) The tomato R gene products I-2 and Mi-1 are functional ATP binding proteins with ATPase activity. Plant Cell, 14, 2929 39.
38. Tameling, W.I.L., Vossen, J.H., Albrecht, M., Lengauer, T., Berden, J.A., Haring, M.A., Cornelissen, B.J.C. Takken, F.L.W. (2006) Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation. Plant Physiol. 140, 1233 45.
39. Tao, Y., Yuan, F.H., Leister, R.T., Ausubel, F.M. Katagiri, F. (2000) Mutational analysis of the Arabidopsis nucleotide binding site-leucine-rich repeat resistance gene RPS2. Plant Cell, 12, 2541 54.
40. Ueda, H., Yamaguchi, Y. Sano, H. (2006) Direct interaction between the tobacco mosaic virus helicase domain and the ATP-bound resistance protein, N factor during the hypersensitive response in tobacco plants. Plant Mol. Biol. 61, 31 45.
41. van't Slot, K.A.E. Knogge, W. (2002) A dual role for microbial pathogen-derived effector proteins in plant disease and resistance. Crit. Rev. Plant. Sci. 21, 229 71.
42. Warren, R.F., Henk, A., Mowery, P., Holub, E. Innes, R.W. (1998) A mutation within the leucine-rich repeat domain of the Arabidopsis disease resistance gene RPS5 partially suppresses multiple bacterial and downy mildew resistance genes. Plant Cell, 10, 1439 52.
43. Winzor, D.J. Scheraga, H.A. (1963) Studies of chemically reacting systems on sephadex 1. Chromatographic demonstration of Gilbert theory. Biochemistry, 2, 1263.