Flow cytometric immunophenotyping of umbilical cord and peripheral blood haematopoietic progenitor cells by different CD34 epitopes, CD133, p-glycoprotein expression and rhodamine-123 efflux

Transfusion Medicine and Hemotherapy

Volumn 34, Issue 3, 2007, Pages 195-203

  Kamprad, Manja ^a   Kindler, Sven ^a   Schuetze, Nicole ^b   Emmrich, Frank ^a  

^a UNIVERSITY HOSPITAL LEIPZIG   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

CD133; CD34 epitopes; Haematopoietic stem cell; P glycoprotein; Rhodamine 123; Umbilical cord blood

Indexed keywords

CD133 ANTIGEN; CD34 ANTIGEN; EPITOPE; GLYCOPROTEIN P; RHODAMINE 123;

ANTIGEN EXPRESSION; ARTICLE; BLOOD TRANSFUSION; CD34 SELECTION; CELL COMPARTMENTALIZATION; CORD BLOOD STEM CELL TRANSPLANTATION; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL; HUMAN; IMMUNOPHENOTYPING; MAJOR HISTOCOMPATIBILITY COMPLEX RESTRICTION; MALIGNANT TRANSFORMATION; MEDICAL ASSESSMENT; NEWBORN; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN EXPRESSION; UMBILICAL CORD;

EID: 34250009732     PISSN: 16603796     EISSN: None     Source Type: Journal    
DOI: 10.1159/000101555     Document Type: Article

References (53)

1. Eichler H, Burkhart J, Sputtek A, Wiesneth M: Stellungnahme der Sektion Transplantation und Zelltherapie der DGTI (15. August 2005) zur Gewinnung und Langzeitlagerung von autologen und allogenen Stammzellpräparaten aus Nabelschnurblut: Indikationen und Grenzen. Transfus Med Hemother 2005;32:274-282.
2. Keeney M, Gratama JW, Sutherland DR: Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts. Cytometry A 2004;58A:72-75.
3. Mittag A, Lenz D, Gerstner AOH, Tárnok A: Hyperchromatic cytometry principles for cytomics using slide based cytometry. Cytometry A 2006;69A:691-703.
4. Tárnok A, Bocsi J, Brockhoff G: Cytomics - importance of multimodal analysis of cell function and proliferation in oncology. Cell Prolif 2006;39:495-505.
5. Bocsi J, Mittag A, Sack U, Gerstner AOH, Barten MJ, Tárnok A: Novel aspects of systems biology and clinical cytomics. Cytometry A 2006;69A:105-108.
6. Steen R, Tjonnfjord GE, Gaudernack G, Brinch L, Egeland T: Differences in the distribution of CD34 epitopes on normal haemopoietic progenitor cells and leukaemic blast cells. Br J Haematol 1996;94:597-605.
7. Steen R, Tjonnfjord GE, Groseth LAG, Egeland T: Characteristics of haemopoietic progenitor cells related to CD34 epitope class expression. Eur J Haematol 2000;64:245-251.
8. Maynadie M, Gerland L, Aho S, Girodon F, Bernier M, Brunet C, Campos L, Daliphard S, Deneys V, Falkenrodt A, Jacob MC, Kuhlein E, LeCalvez G, Moskovtchenko P, Philip P, Carli PM, Faure GC, Bene MC: Clinical value of the quantitative expression of the three epitopes of CD34 in 300 cases of acute myeloid leukemia. Haematologica 2002;87:795-803.
9. Lanza F, Healy L, Sutherland DR: Structural and functional features of the CD34 antigen: an update. J Biol Regul Homeost Agents 2001;15:1-13.
10. Gratama JW, Sutherland DR, Keeney M, Papa S: Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells. J Biol Regul Homeost Agents 2001;15:14-22.
11. Shmelkov SV, St Clair R, Lyden D, Rafii S: AC133/CD133/prominin-1. Int J Biochem Cell Biol 2005;37:715-719.
12. Miraglia S, Godfrey W, Yin AH, Atkins K, Warnke R, Holden JT, Bray RA, Waller EK, Buck DW: A novel five-transmembrane hematopoietic stem cell antigen: Isolation, characterization, and molecular cloning. Blood 1997;90:5013-5021.
13. Hilbe W, Dirnhofer S, Oberwasserlechner F, Schmid T, Gunsilius E, Hilbe G, Woll E, Kahler CM: CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer. J Clin Pathol 2004;57:965-969.
14. Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB: Identification of human brain tumour initiating cells. Nature 2004;432:396-401.
15. de Wynter EA, Durig J, Cross MA, Heyworth CM, Testa NG: Differential response of CD34+ cells isolated from Cord Blood And Bone Marrow to MIP-1α and the expression of MIP-1α Receptors On These Immature Cells. Stem Cells 1998;16:349-356.
16. + cells from human umbilical cord blood. Br J Haematol 2000;108:793-800.
17. Jaatinen T, Hemmoranta H, Hautaniemi S, Niemi J, Nicorici D, Laine J, Yli-Harja O, Partanen J: Global Gene Expression Profile Of Human Cord Blood-Derived CD133+ cells. Stem Cells 2006;24:631-641.
18. Yin AH, Miraglia S, Zanjani ED, AlmeidaPorada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW: AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997;90:5002-5012.
19. Gallacher L, Murdoch B, Wu DM, Karanu FN, Keeney M, Bhatia M: Isolation and characterization of human CD34-Lin- and CD34+Lin- hematopoietic stem cells using cell surface markers AC133 and CD7. Blood 2000;95:2813-2820.
20. + cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential. Blood 2005;105:617-626.
21. Wagner W, Ansorge A, Wirkner U, Eckstein V, Schwager C, Blake J, Miesala K, Selig J, Saffrich R, Ansorge W, Ho AD: Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis. Blood 2004;104:675-686.
22. Kim M, Moon HB, Spangrude GJ: Major age-related changes of mouse hematopoietic stem/progenitor cells. Ann N Y Acad Sci 2003;996:195-208.
23. Bunting KD: ABC transporters as phenotypic markers and functional regulators of stem cells. Stem Cells 2002;20:11-20.
24. Zinovyeva MV, Zijlmans JMJM, Fibbe WE, Visser JWM, Belyavsky AV: Analysis of gene expression in subpopulations of murine hematopoietic stem and progenitor cells. Exp Hematol 2000;28:318-334.
25. Kim MJ, Cooper DD, Hayes SF, Spangrude GJ: Rhodamine-123 staining in hematopoietic stem cells of young mice indicates mitochondrial activation rather than dye efflux. Blood 1998;91:4106-4117.
26. Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I: The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 1996;5:213-226.
27. Law P, Traylor L, Recktenwald DJ: Cell analysis for hematopoietic stem/progenitor cell transplantation. Cytometry 1999;38:47-52.
28. Levering WHBM, Sutherland DR, Keeney M, Kraan J, Gratama JW: Haematopoietic stem and progenitor cells: enumeration, phenotypic characterisation, and clinical applications. Transfus Med Hemother 2004;31:341-352.
29. Kahn J, Byk T, Jansson-Sjostrand L, Petit I, Shivtiel S, Nagler A, Hardan I, Deutsch V, Gazit Z, Gazit D, Karlsson S, Lapidot T: Overexpression of CXCR4 on human CD34+ progenitors increases their proliferation, migration, and NOD/SCID repopulation. Blood 2004;103:2942-2949.
30. Lanza F, Moretti S, Castagnari B, Latorraca A, Rigolin GM, Bardi A, Castoldi G: Cd34+ leukemic-cells assessed by different CD34 monoclonal- antibodies. Leuk Lymphoma 1995;18:25-30.
31. + bone marrow and peripheral blood stem cells. Scand J Immunol 1998;47:82-90.
32. Steen R, Egeland T: CD34 molecule epitope distribution on cells of haematopoietic origin. Leuk Lymphoma 1998;30:23-30.
33. Sovalat H, Racadot E, Henon P, Fuchs P, Lewandowski H, Billot M: Comparative analysis of class I, II and III epitope-detecting CD34 monoclonal antibodies by quantitative flow cytometry. Hematol Cell Ther 1998;40:259-268.
34. Otto M, Chen X, Martin WJ, Leung W, Knowles J, Holladay M, Houston J, Handgretinger R, Barfield RC: Selection of stem cells by using antibodies that target different CD34 epitopes yields different patterns of T-cell differentiation. Stem Cells 2007;25:537-542.
35. + cells. Stem Cells Dev 2006;15:839-851.
36. + cells. Stem Cells 2006;24:631-641.
37. Toren A, Bielorai B, Jacob-Hirsch J, Fisher T, Kreiser D, Moran O, Zeligson S, Givol D, Yitzhaky A, Itskovitz-Eldor J, Kventsel I, Rosenthal E, Amariglio N, Rechavi G: CD133-positive hematopoietic stem cell 'stemness' genes contain many genes mutated or abnormally expressed in leukemia. Stem Cells 2005;23:1142-1153.
38. + umbilical cord blood hematopoietic stem progenitor cells. Stem Cells Dev 2005;14:188-198.
39. + blood stem cells derived from a single donor. Cell Prolif 2006;39:325-332.
40. + hematopoietic progenitor cells. Transfusion 2004;44:1087-1097.
41. Handgretinger R, Gordon PR, Leimig T, Chen XH, Buhring HJ, Niethammer D, Kuci S: Biology and plasticity of CD133+ hematopoietic stem cells. Ann N Y Acad Sci 2003;996:141-151.
42. + selected progenitor cells from alternative donors. Br J Haematol 2004;124:72-79.
43. +-purified stem cells. Exp Hematol 2005;33:713-718.
44. McGuckin CP, Pearce D, Forraz N, Tooze JA, Watt SM, Pettengell R: Multiparametric analysis of immature cell populations in umbilical cord blood and bone marrow. Eu J Haematol 2003;71:341-350.
45. - stem cells that give rise to SCID-repopulating cells. Blood 2003;101:869-876.
46. Baal N, Reisinger K, Jahr H, Bohle RM, Liang O, Munstedt K, Rao CV, Preissner KT, Zygmunt MT: Expression of transcription factor Oct-4 and other embryonic genes in CD133 positive cells from human umbilical cord blood. Thromb Haemost 2004;92:767-775.
47. Tondreau T, Meuleman N, Delforge A, Dejeneffe M, Leroy R, Massy M, Mortier C, Bron D, Lagneaux L: Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: Proliferation, Oct4 expression, and plasticity. Stem Cells 2005;23:1105-1112.
48. Zhou S, Morris JJ, Barnes YX, Lan L, Schuetz JD, Sorrentino BP: Bcrp1 gene expression is required for normal numbers of side population stem cells in mice, and confers relative protection to mitoxantrone in hematopoietic cells in vivo. Proc Natl Acad Sci U S A 2002;99:12339-12344.
49. Uchida N, Dykstra B, Lyons KJ, Leung FY, Eaves CJ: Different in vivo repopulating activities of purified hematopoietic stem cells before and after being stimulated to divide in vitro with the same kinetics. Exp Hematol 2003;31:1338-1347.
50. Gordon PR, Leimig T, Babarin-Dorner A, Houston J, Holladay M, Mueller I, Geiger T, Handgretinger R: Large-scale isolation of CD133+progenitor cells from G-CSF mobilized peripheral blood stem cells. Bone Marrow Transplant 2003;31:17-22.
51. Rosati A, Candussio L, Crivellato E, Klugmann FB, Giraldi T, Damiani D, Michelutti A, Decorti G: Bodipy-FL-verapamil: A fluorescent probe for the study of multidrug resistance proteins. Cell Oncol 2004;26:3-11.
52. Raaijmakers HGP, van den Bosch G, Boezeman J, de Witte T, Raymakers RAP: Single-cell image analysis to assess ABC-transporter-mediated efflux in highly purified hematopoietic progenitors. Cytometry 2002;49:135-142.
53. Taylor BJ, Olson DP, Ivy SP: Detection of P-glycoprotein in cell lines and leukemic blasts: failure of select monoclonal antibodies to detect clinically significant Pgp levels in primary cells. Leuk Res 2001;25:1127-1135.