Parenteral long-acting amoxicillin reduces intestinal bacterial community diversity in piglets even 5 weeks after the administration

ISME Journal

Volumn 1, Issue 2, 2007, Pages 180-183

  Janczyk, Pawel ^a   Pieper, Robert ^a   Souffrant, Wolfgang Bernhard ^a   Bimczok, Diane ^b   Rothkötter, Hermann Josef ^b   Smidt, Hauke ^c  

^a LEIBNIZ INSTITUTE FOR FARM ANIMAL BIOLOGY FBN   (Germany)

^b OTTO VON GUERICKE UNIVERSITY   (Germany)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Amoxicillin; DGGE; Gut microbiota; Piglets

Indexed keywords

AMOXICILLIN; ANTIINFECTIVE AGENT; BACTERIAL DNA; RNA 16S;

ANIMAL; ANIMAL FOOD; ARTICLE; BACTERIUM; CLASSIFICATION; DNA SEQUENCE; DRUG EFFECT; ECOSYSTEM; GENETICS; GROWTH, DEVELOPMENT AND AGING; INTESTINE; INTRAMUSCULAR DRUG ADMINISTRATION; MICROBIOLOGY; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SUCKLING; SWINE;

AMOXICILLIN; ANIMAL FEED; ANIMALS; ANIMALS, SUCKLING; ANTI-BACTERIAL AGENTS; BACTERIA; DNA, BACTERIAL; ECOSYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INJECTIONS, INTRAMUSCULAR; INTESTINES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SWINE;

ANIMALIA; BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; ESCHERICHIA COLI; HORDEUM; LACTOBACILLUS; LACTOBACILLUS SOBRIUS; ROSEBURIA FAECALIS; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHI; SHIGELLA; TRITICUM AESTIVUM;

EID: 34249947608     PISSN: 17517362     EISSN: 17517370     Source Type: Journal    
DOI: 10.1038/ismej.2007.29     Document Type: Article

References (18)

1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W et al. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
2. de la Cochetière MF, Durand T, Lepage P, Bourreille A, Galmiche JP, Doré J. (2005). Resilience of the dominant human fecal microbiota upon short-course antibiotic challenge. J Clin Microbiol 43: 5588-5592.
3. Duncan SH, Hold GI, Barcenilla A, Stewart CS, Flint HI. (2002). Roseburia intestinalis sp. nov., a novel saccharolytic, butyrate-producing bacterium from human faeces'. Int J Syst Evol Microbiol 52: 1615-1620.
4. Duncan SH, Louis P, Flint HI. (2004). Lactate-Utilizing bacteria, isolated from human faeces, that produce butyrate as a major fermentation product. Appl Environ Microbiol 70: 5810-5817.
5. Felske A, Akkermans AD, de Vos WM. (1998). Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis fingerprints. Appl Environ Microbiol 64: 4581-4587.
6. Janczyk P, Pieper R, Smidt H, Souffrant WB. (2007). Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA-gene amplification and denaturing gradient gel electrophoresis. FEMS Microbiol Ecol [Epub ahead of print, 11 April 2007; doi: 10.1111/j.1574-6941.2007.00317.x].
7. Konstantinov SR, Awati A, Smidt H, Williams BA, Akkermans ADL, de Vos WM. (2004). Specific response of a novel and abundant Lactobacillus amylo-vorus-like phylotype to dietary prebiotics in the guts of weaning piglets. Appl Environ Microbiol 70: 3821-3830.
8. Konstantinov SR, Awati AA, Williams BA, Miller BG, Jones P, Stokes CR et al. (2006a). Post-natal development of the porcine microbiota composition and activities. Environ Microbiol 8: 1191-1199.
9. Konstantinov SR, Poznanski E, Fuentes S, Akkermans ADL, Smidt H, de Vos WM. (2006b). Lactobacillus sobrius sp. nov., a novel isolate abundant in the intestine of weaning piglets. Int J Syst Evol Microbiol 56: 29-32.
10. Leeb M. (2004). A shot in the arm. Nature 431: 892-893.
11. Loh G, Eberhard M, Brunner RM, Hennig U, Kuhla S, Kleessen B et al. (2006). Inulin alters the intestinal microbiota and short-chain fatty acid concentration in growing pigs regardless to their basal diet'. J Nutr 136: 1198-1202.
12. McCracken VJ, Simpson JM, Mackie RI, Gaskins HR. (2001). Molecular ecological analysis of dietary and antibiotic-induced alterations of the mouse intestinal microbiota. J Nutr 131: 1862-1870.
13. Muyzer G, Smalla K. (1998). Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Van Leeuwenhoek Int J Gen Mol Microbiol 73: 127-141.
14. Nathan C. (2004). Antibiotics at the crossroads. Nature 431: 899-902.
15. Nübel U, Engelen B, Felske A, Snaidr J, Wieshuber A, Amann R et al. (1996). Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178: 5636-5643.
16. Simpson JM, McCracken VJ, Gaskins HR, Mackie RI. (2000). Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuterii strain MM53. Appl Environ Microbiol 66: 4705-4714.
17. Simpson JM, McCracken VJ, White BA, Gaskins HR, Mackie RI. (1999). Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota. J Microbiol Methods 36: 167-179.
18. Sullivan A, Edlund C, Nord CE. (2001). Effect of antimicrobial agents on the ecological balance of human microflora. Lancet Infect Dis 1: 101-114.