Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis

FEMS Microbiology Ecology

Volumn 61, Issue 1, 2007, Pages 132-140

  Janczyk, Pawel ^a   Pieper, Robert ^a   Smidt, Hauke ^b   Souffrant, Wolfgang Bernhard ^a  

^a LEIBNIZ INSTITUTE FOR FARM ANIMAL BIOLOGY FBN   (Germany)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

16S rRNA gene; Denaturing gradient gel electrophoresis; Lactobacilli diversity; Weaning pig

Indexed keywords

RIBOSOME RNA;

BACTERIUM; DNA; ELECTROKINESIS; ENVIRONMENTAL CHANGE; MICROBIAL COMMUNITY; PIG; POLYMERASE CHAIN REACTION; RNA; SPECIES RICHNESS; WEANING;

AMPLICON; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; GENE AMPLIFICATION; ILEUM; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS SALIVARIUS; LACTOBACILLUS SOBRIUS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SWINE; WEANING;

ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ILEUM; LACTOBACILLUS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SWINE; WEANING;

ANIMALIA; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS SALIVARIUS; LACTOBACILLUS SOBRIUS; SUIDAE; SUS;

EID: 34249937249     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2007.00317.x     Document Type: Article

References (43)

1. Ahrné S, Lönnemark E, Wold AE, Åberg N, Hesselmar B, Saalman R, Strannegård I-L, Molin G Adlerberth I (2005) Lactobacilli in the intestinal microbiota of Swedish infants. Microbes Infect 7:1256 1262.
2. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389 3402.
3. Bailey M, Haverson K, Inman C, Harris C, Jones P, Corfield G, Miller B Stokes C (2005) The influence of environment on development of the mucosal immune system. Vet Immunol Immunopathol 108:189 198.
4. Berg RD (1996) The indigenous gastrointestinal microflora. Trends Microbiol 4:430 435.
5. Bernet MF, Brassart D, Neser JR Servin AL (1994) Lactobacillus acidophilus LA1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut 35:483 489.
6. Bogovič Matijasič B, Narat M, Zorič Peternel M Rogelj I (2006) Ability of Lactobacillus gasseri K7 to inhibit Escherichia coli adhesion in vitro on Caco-2 cells and ex vivo on pigs' jejunal tissue. Int J Food Microbiol 107:92 96.
7. Boudry G, Péron V, le Huërou-Luron I, Lallès P Sève B (2004) Weaning induces both transient and long-lasting modifications of absorptive, secretory and barrier properties of piglet intestine. J Nutr 134:2256 2262.
8. Coconnier MH, Bernet MF, Kernéis S, Chauvière G, Fourniat J Servin AL (1993) Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion. FEMS Microbiol Lett 110:299 306.
9. Collier CT, Smiricky-Tjardes MR, Albin DM, Wubben JE, Gabert VM, Deplancke B, Bane D, Anderson DB Gaskins HR (2003) Molecular ecological analysis of porcine ileal microbiota responses to antimicrobial growth promoters. J Anim Sci 81:3035 3045.
10. Crosby LD Criddle CS (2003) Understanding bias in microbial community analysis techniques due to rrn operon copy number heterogeneity. Biotechniques 34:790 802.
11. Hampson DJ 1986 Alterations in piglet small intestinal structure at weaning. Res Vet Sci 40:32 40.
12. Heilig HGHJ, Zoetendal EG, Vaughan E, Marteau P, Akkermans ADL de Vos WM (2002) Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68:114 123.
13. Kalliomäki M, Salminen S, Arvilommi H, Kero P, Koskinen P Isolauri E (2001) Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial. Lancet North Am Ed 357:1076 1079.
14. Klappenbach JA, Dunbar JM Schmidt TM (2000) rRNA operon copy number reflects ecological strategies of bacteria. Appl Environ Microbiol 66:1328 1333.
15. Kluess J 2004 Evaluation of ileal digesta in weaning piglets fed different diets, PhD thesis, Mensch und Buch Verlag, Berlin.
16. Konstantinov SR Smidt H (2006) Nutrition and Immunity Mengheri E, Britti MS Finamore A, eds), pp. 23 38. Research Signpost, Kerala.
17. Konstantinov SR, Awati A, Smidt H, Williams BA, Akkermans ADL de Vos WM (2004) Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets. Appl Environ Microbiol 70:3821 3830.
18. Konstantinov SR, Smidt H de Vos WM (2005) Representational difference analysis and real time PCR for strain-specific quantification of Lactobacillus sobrius sp. nov. Appl Environ Microbiol 71:7578 7581.
19. Konstantinov SR, Poznanski E, Fuentes S, Akkermans ADL, Smidt H deVos WM (2006a) Lactobacillus sobrius sp.nov., abundant in the intestine of weaning piglets. Int J Syst Evol Microbiol 56:29 32.
20. Konstantinov SR, Awati AA, Williams BA, Miller BG, Jones P, Stokes CR, Akkermans ADL, Smidt H de Vos WM (2006b) Post-natal development of the porcine microbiota composition and activities. Environ Microbiol 8:1191 1199.
21. Loh G, Eberhard M, Brunner RM, Hennig U, Kuhla S, Kleessen B Metges CC (2006) Inulin alters the intestinal microbiota and short-chain fatty acid concentration in growing pigs regardless to their basal diet. J Nutr 136:1198 1202.
22. Lu L Walker A (2001) Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. Am J Clin Nutr 73:1124S 1130S.
23. McCracken VJ, Simpson JM, Mackie RI Gaskins HR (2001) Molecular ecological analysis of dietary and antibiotic-induced alterations of the mouse intestinal microbiota. J Nutr 131:1862 1870.
24. Muyzer G (1999) DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol 2:317 322.
25. Palys T, Nakamura LK Cohan FM (1997) Discovery and classification of ecological diversity in the bacterial world: the role of DNA sequence data. Int J Sys Bacteriol 47:1145 1156.
26. Pieper R, Janczyk P, Schumann R Souffrant WB (2006) The intestinal microflora of piglets around weaning with emphasis on Lactobacilli. Arch Zootech 9:28 40.
27. Rogosa M, Wiseman RF, Mitchell JA, Disraely MN Beaman AJ (1953) Species differentiation of oral lactobacilli from man including descriptions of Lactobacillus salivarius nov spec and Lactobacillus celobiosus nov spec. J Bacteriol 65:681 699.
28. Savijoki K, Ingmer H Varmanen P (2006) Proteolytic systems of lactic acid bacteria. Appl Microbiol Biotechnol 71:394 406.
29. Schroder O, Gerhard R Stein J (2006) Antibiotic-associated diarrhoea. Z Gastroenterol 44:193 204.
30. Settani L, Valmorri S, van Sinderen D, Suzzi G, Paparella A Corsetti A (2006) Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species. Appl Environ Microbiol 72:3793 3796.
31. Shim SB, Verstegen MW, Kim IH, Kwon OS Verdonk JM (2005) Effects of feeding antibiotic-free creep feed supplemented with oligofructose, probiotics or synbiotics to suckling piglets increases the preweaning weight gain and composition of intestinal microbiota. Arch Anim Nutr 59:419 427.
32. Simpson JM, McCracken VJ, White BA, Gaskins HR Mackie RI (1999) Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota. J Microbiol Methods 36:167 179.
33. Simpson JM, McCracken VJ, Gaskins HR Mackie RI (2000) Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuterii strain MM53. Appl Environ Microbiol 66:4705 4714.
34. Spreeuwenberg MAM, Verdonk JMAJ, Gaskins HR Verstegen MWA (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. J Nutr 131:1520 1527.
35. Taras D, Vahjen W, Macha M Simon O (2005) Response of performance characteristics and fecal consistency to long-lasting dietary supplementation with the probiotic strain Bacillus cereus var. toyoi to sows and piglets. Arch Anim Nutr 59:405 417.
36. du Toit M, Dicks LM Holzapfel WH (2001) Taxonomy of obligately homofermentative and facultatively heterofermentative lactobacilli in pig feaces. Lett Appl Microbiol 32:199 204.
37. Vaughan EE, de Vries MC, Zoetendal EG, Ben-Amor K, Akkermans AD de Vos WM (2002) The intestinal LABs. Antonie Leeuwenhoek 82:341 352.
38. Vaughan EE, Heilig HG, Ben-Amor K de Vos WM (2005) Diversity, vitality and activities of intestinal lactic acid bacteria and bifidobacteria assessed by molecular approaches. FEMS Microbiol Rev 29:477 490.
39. Walter J, Hertel C, Tannock GW, Lis CM, Munro K Hammes WP (2001) Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67:2578 2585.
40. Yu Z Morrison M (2004) Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR denaturing gradient gel electrophoresis. Appl Environ Microbiol 70:4800 4806.
41. Zeyner A Boldt E (2006) Effects of a probiotic Enterococcus faecium strain supplemented from birth to weaning on diarrhoea patterns and performance of piglets. J Anim Physiol Anim Nutr (Berl) 90:25 31.
42. Zoetendal EG, Akkermans ADL de Vos WM (1998) Temperature gradient gel electrophoresis analysis of 16 S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 64:3854 3859.
43. Zoetendal EG, Ben-Amor K, Harmsen HJ, Schut F, Akkermans AD de Vos WM (2002) Quantification of uncultured Ruminococcus obeum-like bacteria in human fecal samples by fluorescent in situ hybridization and flow cytometry using 16S rRNA-targeted probes. Appl Environ Microbiol 68:4225 4232.