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Drag swabs were assembled as previously described by Caldwell et al. [16]. with some modification. Briefly, 2 sets of sterile gauze pads (10.2 × 10.2 cm) were tied both at the end (2 m) and at 30 cm above (1.70 m) the end of the string. The assembled swabs were placed in a sterile whirl plastic bag and heated at 60°C overnight in a dry oven. Before use, swabs were wet with skim milk.
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Drag swabs were assembled as previously described by Caldwell et al. [16]. with some modification. Briefly, 2 sets of sterile gauze pads (10.2 × 10.2 cm) were tied both at the end (2 m) and at 30 cm above (1.70 m) the end of the string. The assembled swabs were placed in a sterile whirl plastic bag and heated at 60°C overnight in a dry oven. Before use, swabs were wet with skim milk.
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Samples were enriched with tetrathionate broth (210420, Becton Dickinson, and Co., Sparks, MD; -60 mL), and incubated for 24 h at 37°C, They were then streaked for isolation on novobiocincontaining (20 μ.g/mL) brilliant green agar (BGA, 228530, Becton Dickinson and Co.) plates and incubated for an additional 24 h at 37°C. Plates were examined for the presence or absence of typical lactose-negative colonies of Salmonella. Suspected colonies were serologically evaluated with O antigen (229511, Becton, Dickinson, and Co.).
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Samples were enriched with tetrathionate broth (210420, Becton Dickinson, and Co., Sparks, MD; -60 mL), and incubated for 24 h at 37°C, They were then streaked for isolation on novobiocincontaining (20 μ.g/mL) brilliant green agar (BGA, 228530, Becton Dickinson and Co.) plates and incubated for an additional 24 h at 37°C. Plates were examined for the presence or absence of typical lactose-negative colonies of Salmonella. Suspected colonies were serologically evaluated with O antigen (229511, Becton, Dickinson, and Co.).
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A 950-mL quantity of OA was added to 7.8 L of tap water in the medicator bucket. The OA reached the turkeys at the end of the water line at a final dilution of 1:128
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9 cfu/ mL. Enterobacteriaceae bacteria species were combined and kept in 1 bottle separate from the 2 lactic acid bacteria. All bacteria were maintained at 4°C until their use.
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9 cfu/ mL. Enterobacteriaceae bacteria species were combined and kept in 1 bottle separate from the 2 lactic acid bacteria. All bacteria were maintained at 4°C until their use.
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