Extensive accumulation of influenza virus NS1 protein in the nuclei causes effective viral growth in Vero cells

Microbiology and Immunology

Volumn 51, Issue 5, 2007, Pages 577-580

  Ozaki, Hiroichi ^a   Kida, Hiroshi ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Influenza virus; NS1; Nuclear localization

Indexed keywords

AMINO ACID; NONSTRUCTURAL PROTEIN 1; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LINE; CERCOPITHECIDAE; CONTROLLED STUDY; INFLUENZA VIRUS; NONHUMAN; PROTEIN LOCALIZATION; VERO CELL; VIROGENESIS; VIRUS GENE; VIRUS REPLICATION; VIRUS STRAIN;

CERCOPITHECUS AETHIOPS; ORTHOMYXOVIRIDAE;

EID: 34249076953     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2007.tb03935.x     Document Type: Article

References (18)

1. Alonso-Caplen, F.V., Nemeroff, M.E., Qiu, Y., and Krug, R.M. 1992. Nucleocytoplasmic transport: the influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA. Genes Dev. 6: 255-267.
2. Bergmann, M., Garcia-Sastre, A., Carnero, E., Pehamberger, H., Wolff, K., Palese, P., and Muster, T. 2000. Influenza virus NS1 protein counteracts PKR-mediated inhibition of replication. J. Virol. 74: 6203-6206.
3. Brown, L.E., Hinshaw, V.S., and Webster, R.G. 1983. Antigenic variation in the influenza A virus nonstructural protein, NS1. Virology 130: 134-143.
4. Desmyter, J., Melnick, J.L., and Rawls, W.E. 1968. Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (Vero). J. Virol. 2: 955-961.
5. Fortes, P., Beloso, A., and Ortin, J. 1994. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport. EMBO J. 13: 704-712.
6. Greenspan, D., Krystal, M., Nakada, S., Arnheiter, H., Lyles, D.S., and Palese, P. 1985. Expression of influenza virus NS2 nonstructural protein in bacteria and localization of NS2 in infected eucaryotic cells. J. Virol. 54: 833-843.
7. Greenspan, D., Palese, P., and Krystal, M. 1988. Two nuclear location signals in the influenza virus NS1 nonstructural protein. J. Virol. 62: 3020-3026.
8. Hatada, E., Saito, S., and Fukuda, R. 1999. Mutant influenza viruses with a defective NS1 protein cannot block the activation of PKR in infected cells. J. Virol. 73: 2425-2433.
9. Lu, Y., Qian, X.Y., and Krug, R.M. 1994. The influenza virus NS1 protein: a novel inhibitor of pre-mRNA splicing. Genes Dev. 8: 1817-1828.
10. Monto, A.S., Maassab, H.F., and Bryan, E.R. 1981. Relative efficacy of embryonated eggs and cell culture for isolation of contemporary influenza viruses. J. Clin. Microbiol. 13: 233-235.
11. Ozaki, H., Govorkova, E.A., Li, C., Xiong, X., Webster, R.G., and Webby, R.J. 2004. Generation of high-yielding influenza A viruses in African green monkey kidney (Vero) cells by reverse genetics. J. Virol. 78: 1851-1857.
12. Qian, X.Y., Alonso-Caplen, F., and Krug, R.M. 1994. Two functional domains of the influenza virus NS1 protein are required for regulation of nuclear export of mRNA. J. Virol. 68: 2433-2441.
13. Qiu, Y., and Krug, R.M. 1994. The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). J. Virol. 68: 2425-2432.
14. Qiu, Y., Nemeroff, M., and Krug, R.M. 1995. The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing. RNA 1: 304-316.
15. Smith, E.J., Marie, I., Prakash, A., Garcia-Sastre, A., and Levy, D.E. 2001. IRF3 and IRF7 phosphorylation in virus-infected cells does not require double-stranded RNAdependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein. J. Biol. Chem. 276: 8951-8957.
16. Talon, J., Horvath, C.M., Polley, R., Basler, C.F., Muster, T., Palese, P., and Garcia-Sastre, A. 2000. Activation of interferon regulatory factor 3 is inhibited by the influenza A virus NS1 protein. J. Virol. 74: 7989-7996.
17. Tan, S.L., and Katze, M.G. 1998. Biochemical and genetic evidence for complex formation between the influenza A virus NS1 protein and the interferon-induced PKR protein kinase. J. Interferon Cytokine Res. 18: 757-766.
18. Wang, X., Li, M., Zheng, H., Muster, T., Palese, P., Beg, A.A., and Garcia-Sastre, A. 2000. Influenza A virus NS1 protein prevents activation of NF-kappaB and induction of alpha/beta interferon. J. Virol. 74: 11566-11573.