Mutant influenza viruses with a defective NS1 protein cannot block the activation of PKR in infected cells

Journal of Virology

Volumn 73, Issue 3, 1999, Pages 2425-2433

  Hatada, Eriko ^a   Saito, Sakura ^b   Fukuda, Ryuji ^a  

^a KANAZAWA UNIV SCHOOL OF MEDICINE   (Japan)

^b Natl Inst Hlth   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; INITIATION FACTOR 2; VIRUS RNA;

ARTICLE; AUTOPHOSPHORYLATION; ENZYME ACTIVATION; GENE EXPRESSION; HELA CELL; HUMAN; HUMAN CELL; INFLUENZA VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; RNA BINDING; TEMPERATURE SENSITIVE MUTANT; VIRUS GENOME;

EID: 0032980412     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.3.2425-2433.1999     Document Type: Article

References (41)

1. Haudin, F., C. Bach, S. Cusack, and R. W. H. Ruigok. 1994. Structure of influenza virus RNP. I. Influenza virus nucleoprotein melts secondary structure in panhandle RNA and exposes the bases to the solvent. EMBO J. 13:3159-3165.
2. Chien, C., R. Tejero, V. Huang, D. E. Zimmerman, C. B. Rios, R. M. Krug, and G. T. Monteilon. 1997. A novel RNA-binding motif in influenza A virus non-structural protein 1. Nat. Struct. Biol. 4:891-895.
3. Chou, P. Y., and G. D. Fasman. 1978. Empirical predictions of protein conformation. Annu. Rev. Biochem. 47:251-276.
4. de la Luna, S., P. Fortes, A. Beloso, and J. Ortin. 1995. Influenza virus NS1 protein enhances the rate of translation initiation of viral mRNA. J. Virol. 69:2427-2433.
5. Desselberger, U., V. R. Racaniello, J. J. Zazra, and P. Palese. 1980. The 3′ and 5′-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene 8:315-328.
6. Egorov, A., S. Brandt, S. Sereinig, J. Romanova, B. Ferko, D. Katinger, A. Grassauer, G. Alexandrova, H. Katinger, and T. Muster. 1998. Transfectant influenza A viruses with long deletions in the NS1 protein grow efficiently in Vero cells. J. Virol. 72:5437-6441.
7. Enami, K., T. A. Sato, S. Nakada, and M. Enami. 1994. Influenza virus NS1 protein stimulates translation of the M1 protein. J. Virol. 68:1432-1437.
8. Fortes, P., A. Beloso, and J. Ortin. 1994. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport. EMBO J. 13;704-712.
9. Galabru, J., and A. Hovanessian. 1987. Autophosphorylation of the protein kinase dependent on double-stranded RNA. J. Biol. Chem. 262:15538-15544.
10. Hatada, E., M. Hasegawa, K. Shimizu, M. Hatanaka, and R. Fukuda. 1990. Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8. J. Gen. Virol. 71:1283-1292.
11. Hatada, E., T. Takizawa, and R. Fukuda. 1992. Specific binding of influenza A virus NS1 protein to the virus minus-sense RNA in vitro. J. Gen. Virol. 73:17-25.
12. Hatada, E., and R. Fukuda. 1992. Binding of influenza A virus NS1 protein to dsRNA in vitro. J. Gen. Virol. 73:3325-3329.
13. Hatada, E., S. Saito, N. Okisio, and R. Fukuda. 1997. Binding of the influenza virus NS1 protein to model genome RNAs. J. Gen. Virol. 78:1059-1063.
14. Hsu, M-T., J. D. Parvin, S. Gupta, M. Krystal, and P. Palese. 1987. Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. Proc. Natl. Acad. Sci. USA 84: 8140-8144.
15. I protein kinase. J. Virol. 62:3710-3717.
16. Katze, M. G. 1992. The war against the interferon-induced dsRNA-activated protein kinase: can viruses win? J. Interferon Res. 12:241-248.
17. Kobayashi, M, T. Toyoda, D. M. Adyshev, Y. Azuma, and A. Ishihama. 1994. Molecular dissection of influenza virus nucleoprotein: deletion mapping of the RNA binding domain. J. Virol. 68:8433-8436.
18. Konieezny, A., and B. Safer. 1983. Purification of the eukaryotic initiation factor 2-eukaryotic initiation factor 2B complex and characterization of its guanine nucleotide exchange activity during protein synthesis initiation. J. Biol. Chem. 258:3402-3408.
19. Kunkel, T. A., J. D. Roberts, and R. A. Zakour. 1987 Rapid and efficient site-specific mutagencsis without phenotypic selection. Methods Enzymol. 154:367-382.
20. Lamb, R. A. 1989. Genes and proteins of the influenza viruses, p. 1-87. In R. M. Krug (ed.). The influenza viruses. Plenum Press, New York, N.Y.
21. Lee, T. G., J. Tomita, A. G. Hovanessian, and M. G. Katze. 1992. Characterization and regulation of the 58,000-dalton cellular inhibitor of the interferon-induced, dsRNA-activated protein kinase. J. Biol. Chem. 267:14238-14243.
22. Lu, Y., M. Wambach, M. G. Katze, and R. M. Krug. 1995. Binding of the influenza vims NS1 protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the eIF-2 translation initiation factor. Virology 214:222-228.
23. Manche, L., S. R. Green, C. Schmedt, and M. B. Mathews. 1992. Interactions between double-stranded RNA regulators and the protein kinase DAI. Mol. Cell. Biol. 12:5238-5248.
24. Marion, H. M., T. Aragon, A. Beloso, A. Nielo, and J. Ortin. 1997. The N-terminal half of the influenza virus NS1 protein is sufficient for nuclear retention of mRNA and enhancement of viral mRNA translation. Nucleic Acids Res. 25:4271-4277.
25. Marion, R. M., T. Zurcher, S. de la Luna, and J. Ortin. 1997. Influenza virus NS1 protein interacts with viral transcription-replication complexes in vim. J. Gen. Viral. 78:2447-2451.
26. O'Neill, R. E., J. Talon, and P. Palese. 1998. The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins. EMBO J. 17:288-296.
27. Park, Y. W., and M. G. Katze. 1995. Translational control by influenza virus. J. Biol. Chem. 270:28433-28439.
28. Qian, X.-Y., F. Alonso-Capien, and R. M. Krug. 1994. Two functional domains of the influenza virus NS1 protein are required for regulation of nuclear export of mRNA. J. Virol. 68:2433-2441.
29. Qian, X.-Y., C.-Y. Chien, Y. Lu, G. T. Montelione, and R. M. Krug. 1995. An amino-terminal polypeptide fragment of the influenza virus NS1 protein possesses specific RNA-binding activity and largely helical hackhone structure. RNA 1:948-956.
30. Qiu, Y., and R. M. Krug. 1994. The influenza virus NS1 protein is a poly(A)-hinding protein that inhibits nuclear export of mRNAs containing poly(A). J. Virol. 68:2425-2432.
31. Qiu, Y., M. Nemeroff, and R. M. Krug. 1995. The influenza virus NS1 protein hinds to a specific region in human U6-U2 and U6-U4 sn RNA interactions during splicing. RNA 1:304-316.
32. Richardson, J. C., and R. K. Akkina. 1991. NS2 protein of influenza virus is found in purified virus and phosphorylated in infected cells. Arch. Virol. 116:69-80.
33. Ruigrok, R. W. H., and F. Baudin. 1995. Structure of influenza virus ribonucleoprotein particles. II. Purified RNA-free influenza virus ribonucleoprotein forms structures that are indistinguishable from the intact influenza virus ribonucleoprotein particles. J. Gen. Virol. 76:1009-1014.
34. Saito, S. 1989. Possible involvement of virus-induced protein kinase in the antiviral state induced with interferon-γ against Sindhis virus. J. Interferon Res. 9:23-34.
35. Samuel, C. E., G. S. Knutsonk, M. J. Berry, J. A. Atwater, and S. R. Lasky. 1986. Purification of double-stranded RNA-dependent protein kinase from mouse fibroblasts. Methods Enzymol. 119:499-517.
36. Samuel, C. E. 1993. The eIF2 protein kinases, regulators of translation in eucaryotes from yeasts to humans. J. Biol. Chem. 268:7603-7606.
37. Shimizu, K., M. G. Mullinix, R. M. Chanock, and B. R. Murphy. 1982. Temperature-sensitive mutants of influenza A/Udorn/72 (H3N2) virus. II. Genetic analysis and demonstration of intrasegmental complementation. Virology 117:45-61.
38. Skorko, R., D. F. Summers, and J. M. Galarza. 1991. Influenza virus in vitro transcription: roles of NS1 and NP proteins in regulating RNA synthesis. Virology 180:668-677.
39. Smith, D. B., and K. S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transfcrasc. Gene 67:31-40.
40. Staehli, P. 1990. Interferon-induced proteins and the antiviral state. Adv. Virus Res. 33:147-200.
41. Yasuda, J., S. Nakada, A. Kato, T. Toyoda, and A. Ishihama. 1993. Molecular assembly of influenza virus: association of the NS2 protein with virion matrix. Virology 196:249-255.