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Volumn 81, Issue 3, 2007, Pages 624-633

The addition of biphasic calcium phosphate to porous chitosan scaffolds enhances bone tissue development in vitro

Author keywords

Bone tissue engineering; Chitosan; In vitro; Osteoblastic differentiation; Scaffold

Indexed keywords

BONE; CALCIUM PHOSPHATE; GROWTH KINETICS; PRECIPITATION (CHEMICAL); SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING; X RAY SPECTROSCOPY;

EID: 34249054897     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.31010     Document Type: Article
Times cited : (67)

References (62)
  • 1
    • 0036323143 scopus 로고    scopus 로고
    • Formation of three dimensional cell/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor
    • Sikavitsas VI, Bancroft GN, Mikos AG. Formation of three dimensional cell/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor. J Biomed Mater Res 2002;62:136-148.
    • (2002) J Biomed Mater Res , vol.62 , pp. 136-148
    • Sikavitsas, V.I.1    Bancroft, G.N.2    Mikos, A.G.3
  • 2
    • 0033696853 scopus 로고    scopus 로고
    • Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds
    • Shea LD, Wang D, Franceshi RT, Mooney DJ. Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds. Tissue Eng 2000;6:605-617.
    • (2000) Tissue Eng , vol.6 , pp. 605-617
    • Shea, L.D.1    Wang, D.2    Franceshi, R.T.3    Mooney, D.J.4
  • 3
    • 0035255365 scopus 로고    scopus 로고
    • Engineering new bone tissue in vitro on highly porous poly(α-hydroxyl acids)/ hydroxyapatite composite scaffolds
    • Ma PX, Zhang RY, Xiao GZ, Franceschi R. Engineering new bone tissue in vitro on highly porous poly(α-hydroxyl acids)/ hydroxyapatite composite scaffolds. J Biomed Mater Res 2001; 54:284-293.
    • (2001) J Biomed Mater Res , vol.54 , pp. 284-293
    • Ma, P.X.1    Zhang, R.Y.2    Xiao, G.Z.3    Franceschi, R.4
  • 4
    • 0034765279 scopus 로고    scopus 로고
    • Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition
    • Zeltinger J, Sherwood JK, Graham DA, Mueller R, Griffith LG. Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng 2001;7:557-572.
    • (2001) Tissue Eng , vol.7 , pp. 557-572
    • Zeltinger, J.1    Sherwood, J.K.2    Graham, D.A.3    Mueller, R.4    Griffith, L.G.5
  • 5
    • 0035813711 scopus 로고    scopus 로고
    • Topical formulations and wound healing applications of chitosan
    • Ueno H, Mori T, Fujinaga T. Topical formulations and wound healing applications of chitosan. Adv Drug Deliv Rev 2001;52: 105-115.
    • (2001) Adv Drug Deliv Rev , vol.52 , pp. 105-115
    • Ueno, H.1    Mori, T.2    Fujinaga, T.3
  • 8
    • 0037022695 scopus 로고    scopus 로고
    • Control of wound infections using a bilayer chitosan wound dressing with sustainable antibiotic delivery
    • Mi FL, Wu YB, Shyu SS, Schoung JY, Huang YB, Tsai YH, Hao JY. Control of wound infections using a bilayer chitosan wound dressing with sustainable antibiotic delivery. J Biomed Mater Res 2001;59:438-449.
    • (2001) J Biomed Mater Res , vol.59 , pp. 438-449
    • Mi, F.L.1    Wu, Y.B.2    Shyu, S.S.3    Schoung, J.Y.4    Huang, Y.B.5    Tsai, Y.H.6    Hao, J.Y.7
  • 9
    • 0034018067 scopus 로고    scopus 로고
    • Drug release behavior of beads and microgranules of chitosan
    • Gupta KC, Ravi Kumar MN. Drug release behavior of beads and microgranules of chitosan. Biomaterials 2000;21:1115-1119.
    • (2000) Biomaterials , vol.21 , pp. 1115-1119
    • Gupta, K.C.1    Ravi Kumar, M.N.2
  • 10
    • 0037122731 scopus 로고    scopus 로고
    • Lee J, Nam S, Im S, Park Y, Lee Y, Seol Y, Chung C, Lee S. Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials. J Control Release 2002;78: 187-197
    • Lee J, Nam S, Im S, Park Y, Lee Y, Seol Y, Chung C, Lee S. Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials. J Control Release 2002;78: 187-197.
  • 11
    • 0242352525 scopus 로고    scopus 로고
    • Le Tien C, Lacroix M, Ispas-Szabo P, Mateescu MA. N-acylated chitosan: Hydrophobic matrices for controlled drug release. J Control Release 2003;93:1-13
    • Le Tien C, Lacroix M, Ispas-Szabo P, Mateescu MA. N-acylated chitosan: Hydrophobic matrices for controlled drug release. J Control Release 2003;93:1-13.
  • 12
    • 0025965343 scopus 로고
    • In vitro properties of a chitosan-bonded hydroxyapatite bone-filling paste
    • Ito M. In vitro properties of a chitosan-bonded hydroxyapatite bone-filling paste. Biomaterials 1991;12:41-45.
    • (1991) Biomaterials , vol.12 , pp. 41-45
    • Ito, M.1
  • 15
    • 0035998390 scopus 로고    scopus 로고
    • Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants
    • Zhang Y, Zhang MQ. Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants. J Biomed Mater Res 2002;61:1-8.
    • (2002) J Biomed Mater Res , vol.61 , pp. 1-8
    • Zhang, Y.1    Zhang, M.Q.2
  • 16
    • 0034672873 scopus 로고    scopus 로고
    • Application of chitosan-based polysaccharide materials in cartilage tissue engineering: A review
    • Suh JKF, Matthew HWT. Application of chitosan-based polysaccharide materials in cartilage tissue engineering: A review. Biomaterials 2000;21:2589-2598.
    • (2000) Biomaterials , vol.21 , pp. 2589-2598
    • Suh, J.K.F.1    Matthew, H.W.T.2
  • 17
    • 0036892089 scopus 로고    scopus 로고
    • Potential use of chitosan as a cell scaffold material for cartilage tissue engineering
    • Nettles DL, Elder SH, Gilbert JA. Potential use of chitosan as a cell scaffold material for cartilage tissue engineering. Tissue Eng 2002;8:1009-1016.
    • (2002) Tissue Eng , vol.8 , pp. 1009-1016
    • Nettles, D.L.1    Elder, S.H.2    Gilbert, J.A.3
  • 19
    • 0042658021 scopus 로고    scopus 로고
    • Kim SE, Park JH, Cho YW, Chung H, Jeong SY, Lee EB, Kwon IC. Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1: Implications for cartilage tissue engineering. J Control Release 2003;91:365-374
    • Kim SE, Park JH, Cho YW, Chung H, Jeong SY, Lee EB, Kwon IC. Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1: Implications for cartilage tissue engineering. J Control Release 2003;91:365-374.
  • 21
    • 0346754916 scopus 로고    scopus 로고
    • Preparation and characterization of macroporous chitosan-gelatin β-tricalcium phosphate composite scaffolds for bone tissue engineering
    • Yin YJ, Ye F, Cui JF, Zhang FJ, Li XL, Yao KD. Preparation and characterization of macroporous chitosan-gelatin β-tricalcium phosphate composite scaffolds for bone tissue engineering. J Biomed Mater Res A 2003;67:844-855.
    • (2003) J Biomed Mater Res A , vol.67 , pp. 844-855
    • Yin, Y.J.1    Ye, F.2    Cui, J.F.3    Zhang, F.J.4    Li, X.L.5    Yao, K.D.6
  • 23
    • 0019447856 scopus 로고
    • Calcium phosphate ceramics as hard tissue prosthetics
    • Jarcho M. Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop 1981;157:257-278.
    • (1981) Clin Orthop , vol.157 , pp. 257-278
    • Jarcho, M.1
  • 24
    • 0036166394 scopus 로고    scopus 로고
    • Properties of osteoconductive biomaterials: Calcium phosphates
    • LeGeros RZ. Properties of osteoconductive biomaterials: Calcium phosphates. Clin Orthop 2002:395;81-98.
    • (2002) Clin Orthop , vol.395 , pp. 81-98
    • LeGeros, R.Z.1
  • 25
    • 0032723976 scopus 로고    scopus 로고
    • Bioactive ceramics: The effect of surface reactivity on bone formation and bone cell function
    • Ducheyne P, Qiu Q. Bioactive ceramics: The effect of surface reactivity on bone formation and bone cell function. Biomaterials 1999;20:2287-2303.
    • (1999) Biomaterials , vol.20 , pp. 2287-2303
    • Ducheyne, P.1    Qiu, Q.2
  • 26
    • 0033843085 scopus 로고    scopus 로고
    • Morphogenesis and tissue engineering of bone and cartilage: Inductive signals, stem cells, and biomimetic biomaterials
    • Reddi AH. Morphogenesis and tissue engineering of bone and cartilage: Inductive signals, stem cells, and biomimetic biomaterials. Tissue Eng 2000;6:351-359.
    • (2000) Tissue Eng , vol.6 , pp. 351-359
    • Reddi, A.H.1
  • 27
    • 0034672872 scopus 로고    scopus 로고
    • Hutchmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000;21:2529-2543.
    • Hutchmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000;21:2529-2543.
  • 28
    • 1642354939 scopus 로고    scopus 로고
    • Cell growth and function on calcium phosphate reinforced chitosan scaffolds
    • Zhang Y, Zhang M. Cell growth and function on calcium phosphate reinforced chitosan scaffolds. J Mater Sci: Mater Med 2004;15:255-260.
    • (2004) J Mater Sci: Mater Med , vol.15 , pp. 255-260
    • Zhang, Y.1    Zhang, M.2
  • 29
    • 0034040355 scopus 로고    scopus 로고
    • Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite
    • Peter SJ, Lu L, Kim DJ, Mikos AG. Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite. Biomaterials 2000;21: 1207-1213.
    • (2000) Biomaterials , vol.21 , pp. 1207-1213
    • Peter, S.J.1    Lu, L.2    Kim, D.J.3    Mikos, A.G.4
  • 30
    • 0036320618 scopus 로고    scopus 로고
    • In vivo evaluation of a bioactive scaffold for bone tissue engineering
    • Livingstone T, Duscheyne P, Garino J. In vivo evaluation of a bioactive scaffold for bone tissue engineering. J Biomed Mater Res 2002;62:1-13.
    • (2002) J Biomed Mater Res , vol.62 , pp. 1-13
    • Livingstone, T.1    Duscheyne, P.2    Garino, J.3
  • 31
    • 0034908229 scopus 로고    scopus 로고
    • The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair
    • Tamura S, Kataoka H, Matsui Y, Shionoya Y, Ohno K, Michi K-I, Takahashi K, Yamaguchi A. The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair. Bone 2001;29:169-175.
    • (2001) Bone , vol.29 , pp. 169-175
    • Tamura, S.1    Kataoka, H.2    Matsui, Y.3    Shionoya, Y.4    Ohno, K.5    Michi, K.-I.6    Takahashi, K.7    Yamaguchi, A.8
  • 32
    • 0032706448 scopus 로고    scopus 로고
    • Stem cell technology and bioceramics: From cell to gene engineering
    • Ohgushi H, Caplan AI. Stem cell technology and bioceramics: From cell to gene engineering. J Biomed Mater Res Appl Biomater 1999;48:913-927.
    • (1999) J Biomed Mater Res Appl Biomater , vol.48 , pp. 913-927
    • Ohgushi, H.1    Caplan, A.I.2
  • 33
    • 0344584399 scopus 로고    scopus 로고
    • Tissue engineering for bone regeneration using differentiated alveolar bone cells in collagen scaffolds
    • Xiao Y, Qian H, Young WG, Bartold PM. Tissue engineering for bone regeneration using differentiated alveolar bone cells in collagen scaffolds. Tissue Eng 2003;13:1167-1177.
    • (2003) Tissue Eng , vol.13 , pp. 1167-1177
    • Xiao, Y.1    Qian, H.2    Young, W.G.3    Bartold, P.M.4
  • 35
    • 2842576198 scopus 로고
    • Determination of the viscometric constants for chitosan
    • Roberts GAF, Domszy JG. Determination of the viscometric constants for chitosan. Int J Biol Macromol 1982;4:374-377.
    • (1982) Int J Biol Macromol , vol.4 , pp. 374-377
    • Roberts, G.A.F.1    Domszy, J.G.2
  • 36
    • 0033151411 scopus 로고    scopus 로고
    • Porous chitosan scaffolds for tissue engineering
    • Madihally SV, Matthew HWT. Porous chitosan scaffolds for tissue engineering. Biomaterials 1999;20:1133-1142.
    • (1999) Biomaterials , vol.20 , pp. 1133-1142
    • Madihally, S.V.1    Matthew, H.W.T.2
  • 37
    • 34249043745 scopus 로고    scopus 로고
    • Balian G, Wang G-J, Diduch D, Hahn C to Musculoskeletal Development Enterprises, LLC, Pluripotential bone marrow cell line and methods of using the same. US Patent 6,082,364, December 15, 1997
    • Balian G, Wang G-J, Diduch D, Hahn C (to Musculoskeletal Development Enterprises, LLC). Pluripotential bone marrow cell line and methods of using the same. US Patent 6,082,364, December 15, 1997.
  • 38
    • 0032983542 scopus 로고    scopus 로고
    • Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential
    • Wang D, Christensen K, Chawla K, Xiao G, Krebsbach PH, Franceschi RT. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 1999; 14:893-903.
    • (1999) J Bone Miner Res , vol.14 , pp. 893-903
    • Wang, D.1    Christensen, K.2    Chawla, K.3    Xiao, G.4    Krebsbach, P.H.5    Franceschi, R.T.6
  • 39
    • 0021061819 scopus 로고
    • Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
    • Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
    • (1983) J Immunol Methods , vol.65 , pp. 55-63
    • Mosmann, T.1
  • 40
    • 0000769015 scopus 로고    scopus 로고
    • Osteoblasts
    • Koller MR, Palsson BO, Masters JRW, editors, Norwell, MA: Kluwer;
    • Di-Silvio L, Gurav N. Osteoblasts. In: Koller MR, Palsson BO, Masters JRW, editors. Primary Mesenchymal Cells. Norwell, MA: Kluwer; 2001. pp 221-241.
    • (2001) Primary Mesenchymal Cells , pp. 221-241
    • Di-Silvio, L.1    Gurav, N.2
  • 41
    • 0026537256 scopus 로고
    • Concepts of osteoblast growth and differentiation - Basis for modulation of bone cell-development and tissue formation
    • Lian JB, Stein GS. Concepts of osteoblast growth and differentiation - Basis for modulation of bone cell-development and tissue formation. Crit Rev Oral Biol Med 1992;3:269-305.
    • (1992) Crit Rev Oral Biol Med , vol.3 , pp. 269-305
    • Lian, J.B.1    Stein, G.S.2
  • 42
    • 0025085822 scopus 로고
    • Relationship of cell-growth to the regulation of tissue-specific gene-expression during osteoblast differentiation
    • Stein GS, Lian JB, Owen TA. Relationship of cell-growth to the regulation of tissue-specific gene-expression during osteoblast differentiation. FASEB J 1990;4:3111-3123.
    • (1990) FASEB J , vol.4 , pp. 3111-3123
    • Stein, G.S.1    Lian, J.B.2    Owen, T.A.3
  • 43
    • 0029061758 scopus 로고
    • Molecular-biology of matrix vesicles
    • Anderson HC. Molecular-biology of matrix vesicles. Clin Orthop 1995;314:266-280.
    • (1995) Clin Orthop , vol.314 , pp. 266-280
    • Anderson, H.C.1
  • 44
    • 80051993203 scopus 로고    scopus 로고
    • Bone mineralization
    • Cowin SC, editor, Boca Raton, FL: CRC Press;
    • Boskey AL. Bone mineralization. In: Cowin SC, editor. Bone Mechanics Handbook. Boca Raton, FL: CRC Press; 2001. pp 5.1-5.33.
    • (2001) Bone Mechanics Handbook
    • Boskey, A.L.1
  • 47
    • 0031914768 scopus 로고    scopus 로고
    • Effects of extracellular calcium on the proliferation and differentiation of porcine osteoblasts in vitro
    • Eklou-Kalonji E, Denis I, Lieberherr M, Pointillart A. Effects of extracellular calcium on the proliferation and differentiation of porcine osteoblasts in vitro. Cell Tissue Res 1998;292:163-171.
    • (1998) Cell Tissue Res , vol.292 , pp. 163-171
    • Eklou-Kalonji, E.1    Denis, I.2    Lieberherr, M.3    Pointillart, A.4
  • 48
    • 0030891960 scopus 로고    scopus 로고
    • A distinct cation-sensing mechanism in MC3T3-E1 osteoblasts functionally related to the calcium receptor
    • Quarles LD, Hartle JE, Siddhanti SR, Guo R, Hinson TK. A distinct cation-sensing mechanism in MC3T3-E1 osteoblasts functionally related to the calcium receptor. J Bone Miner Res 1997;12:393-402.
    • (1997) J Bone Miner Res , vol.12 , pp. 393-402
    • Quarles, L.D.1    Hartle, J.E.2    Siddhanti, S.R.3    Guo, R.4    Hinson, T.K.5
  • 49
    • 0035138842 scopus 로고    scopus 로고
    • Extracellular calcium sensing and extracellular calcium signaling
    • Brown EM, MacLeod RJ. Extracellular calcium sensing and extracellular calcium signaling. Physiol Rev 2001;81:239-297.
    • (2001) Physiol Rev , vol.81 , pp. 239-297
    • Brown, E.M.1    MacLeod, R.J.2
  • 50
    • 0023882543 scopus 로고
    • Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts
    • Silver IA, Murrills RJ, Etherington DJ. Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts. Exp Cell Res 1988;175:266-276.
    • (1988) Exp Cell Res , vol.175 , pp. 266-276
    • Silver, I.A.1    Murrills, R.J.2    Etherington, D.J.3
  • 51
    • 0037039862 scopus 로고    scopus 로고
    • Third-generation biomedical materials
    • Hench LL, Polak JM. Third-generation biomedical materials. Science 2002;295:1016,1017.
    • (2002) Science , vol.295 , pp. 1016-1017
    • Hench, L.L.1    Polak, J.M.2
  • 52
    • 0033622838 scopus 로고    scopus 로고
    • Growth of continuous bonelike mineral within porous poly(lactide-co-glycolide) scaffolds in vitro
    • Murphy WL, Kohn DH, Mooney DJ. Growth of continuous bonelike mineral within porous poly(lactide-co-glycolide) scaffolds in vitro. J Biomed Mater Res 2000;50:50-58.
    • (2000) J Biomed Mater Res , vol.50 , pp. 50-58
    • Murphy, W.L.1    Kohn, D.H.2    Mooney, D.J.3
  • 53
    • 3242681610 scopus 로고    scopus 로고
    • The effect of biomimetic apatite structure on osteoblast viability, proliferation, and gene expression
    • Chou YF, Huang WB, Dunn JCY, Miller TA, Wu BM. The effect of biomimetic apatite structure on osteoblast viability, proliferation, and gene expression. Biomaterials 2005;26:285-295.
    • (2005) Biomaterials , vol.26 , pp. 285-295
    • Chou, Y.F.1    Huang, W.B.2    Dunn, J.C.Y.3    Miller, T.A.4    Wu, B.M.5
  • 54
    • 0033563980 scopus 로고    scopus 로고
    • Porous poly(L-lactic acid)/apatite composites created by biomimetic process
    • Zhang RY, Ma PX. Porous poly(L-lactic acid)/apatite composites created by biomimetic process. J Biomed Mater Res 1999;45: 285-293.
    • (1999) J Biomed Mater Res , vol.45 , pp. 285-293
    • Zhang, R.Y.1    Ma, P.X.2
  • 55
    • 0036132476 scopus 로고    scopus 로고
    • Initial attachment of osteoblasts to an optimised HAPEX™ topography
    • Dalby MJ, Kayser MV, Bonfield W, Di Silvio L. Initial attachment of osteoblasts to an optimised HAPEX™ topography. Biomaterials 2002;23:681-690.
    • (2002) Biomaterials , vol.23 , pp. 681-690
    • Dalby, M.J.1    Kayser, M.V.2    Bonfield, W.3    Di Silvio, L.4
  • 56
    • 0032960529 scopus 로고    scopus 로고
    • High resolution morphometric analysis of human osteoblastic cell adhesion on clinically relevant orthopedic alloys
    • Shah AK, Sinha RK, Hickok NJ, Tuan RS. High resolution morphometric analysis of human osteoblastic cell adhesion on clinically relevant orthopedic alloys. Bone 1999;24:499-506.
    • (1999) Bone , vol.24 , pp. 499-506
    • Shah, A.K.1    Sinha, R.K.2    Hickok, N.J.3    Tuan, R.S.4
  • 57
    • 0033961642 scopus 로고    scopus 로고
    • Osteoblast adhesion on biomaterials
    • Anselme K. Osteoblast adhesion on biomaterials. Biomaterials 2000;21:667-681.
    • (2000) Biomaterials , vol.21 , pp. 667-681
    • Anselme, K.1
  • 58
    • 1842426730 scopus 로고    scopus 로고
    • Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    • McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 2004;6:483-495.
    • (2004) Dev Cell , vol.6 , pp. 483-495
    • McBeath, R.1    Pirone, D.M.2    Nelson, C.M.3    Bhadriraju, K.4    Chen, C.S.5
  • 59
    • 0037542854 scopus 로고    scopus 로고
    • Ras proteins: Different signals from different locations
    • Hancock JF. Ras proteins: Different signals from different locations. Nat Rev Mol Cell Biol 2003;4:373-384.
    • (2003) Nat Rev Mol Cell Biol , vol.4 , pp. 373-384
    • Hancock, J.F.1
  • 61
    • 0028961293 scopus 로고
    • Rho, Rac, and Cdc42 Gtpases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
    • Nobes CD, Hall A. Rho, Rac, and Cdc42 Gtpases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 1995;81:53-62.
    • (1995) Cell , vol.81 , pp. 53-62
    • Nobes, C.D.1    Hall, A.2
  • 62
    • 0036131693 scopus 로고    scopus 로고
    • Three-dimensional reconstruction of confocal laser microscopy images to study the behaviour of osteoblastic cells grown on biomaterials
    • Ramires PA, Giuffrida A, Mirella E. Three-dimensional reconstruction of confocal laser microscopy images to study the behaviour of osteoblastic cells grown on biomaterials. Biomaterials 2002;23:397-406.
    • (2002) Biomaterials , vol.23 , pp. 397-406
    • Ramires, P.A.1    Giuffrida, A.2    Mirella, E.3


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