Purification and characterization of aminopeptidase N from Spodoptera litura expressed in Sf21 insect cells

Protein Expression and Purification

Volumn 54, Issue 2, 2007, Pages 267-274

  Kaur, Ravinder ^a   Agrawal, Neema ^a   Bhatnagar, Raj ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

Author keywords

Aminopeptidase N; Detergent; Purification; Sf21 insect cells; Solubilisation; Spodoptera litura

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; MICROSOMAL AMINOPEPTIDASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; ARMYWORM; ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN ANALYSIS; PROTEIN FOLDING; SOLUBILITY;

ANIMALS; ANTIGENS, CD13; BACTERIAL PROTEINS; BACTERIAL TOXINS; ENDOTOXINS; HEMOLYSIN PROTEINS; PROTEIN FOLDING; PROTEIN INTERACTION MAPPING; RECOMBINANT PROTEINS; SOLUBILITY; SPODOPTERA;

BACILLUS THURINGIENSIS; HEXAPODA; SPODOPTERA LITURA;

EID: 34249026671     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.03.003     Document Type: Article

References (35)

1. Schnepf E., Crickmore N., Van Rie J., Lereclus D., Baum J., Feitelson J., Zeigler D.R., and Dean D.H. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62 (1998) 775-806
2. Aronson I., and Shai Y. Why Bacillus thuringiensis insecticidal toxins are so effective: unique features of their mode of action. FEMS Microbiol. Lett. 195 (2001) 1-8
3. Hofmann C., Vanderbruggen H., Hofte H., Van R.J., Jansens S., and Mellaert H.V. Specificity of Bacillus thuringiensis δ-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midgets. Proc. Natl. Acad. Sci. USA 85 (1988) 7844-7848
4. Ihara H., Kuroda E., Wadano A., and Himeno M. Specific toxicity of δ-endotoxins from Bacillus thuringiensis to Bombyx mori. Biosci. Biotechnol. Biochem. 57 (1993) 200-204
5. Rajamohan F., Alcantara E., Lee M.K., Chen X.J., Curtiss A., and Dean D.H. Single amino acid changes in domain II of Bacillus thuringiensis Cry1Ab δ-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles. J. Bacteriol. 177 (1995) 2276-2282
6. Van R.J., Jansens S., Hofte H., Degheele D., and Mellaert H. Specificity of Bacillus thuringiensis delta-endotoxins: importance of specific receptors on the brush border membrane of the midgut of target insects. Eur. J. Biochem. 186 (1989) 239-247
7. Agrawal N., Malhotra P., and Bhatnagar R.K. Interaction of gene-cloned and insect cell-expressed aminopeptidase N of Spodoptera litura with insecticidal crystal protein Cry1C. Appl. Environ. Microbiol. 68 (2002) 4583-4592
8. Lee M., You T., Young B., Cotrill J., Valaitis A., and Dean D.H. Aminopeptidase N purified from gypsy moth brush border membrane vesicles is a specific receptor for Bacillus thuringiensis CryIAc toxin. Appl. Environ. Microbiol. 62 (1996) 2845-2849
9. Luo K., Sangadala S., Masson L., Mazza A., Brousseau R., and Adang M.J. The Heliothis virescens 170 kDa aminopeptidase functions as "Receptor A" by mediating specific Bacillus thuringiensis Cry1A δ-endotoxin binding and pore formation. Insect Biochem. Mol. Biol. 27 (1997) 735-743
10. + efflux in vitro. J. Biol. Chem. 269 (1994) 10088-10092
11. Gill M., and Ellar D. Transgenic Drosophila reveals a functional in vivo receptor for the Bacillus thuringiensis toxin Cry1Ac1. Insect Mol. Biol. 11 (2002) 619-625
12. Rajagopal R., Sivakumar S., Agrawal N., Malhotra P., and Bhatnagar R.K. Silencing of midgut aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor. J. Biol. Chem. 277 (2002) 46849-46851
13. Garczynski S.F., and Adang M.J. Bacillus thuringiensis CryIA(c) δ-endotoxin binding aminopeptidase in the Manduca sexta midgut has a glycosyl-phosphatidylinositol anchor. Insect Biochem. Mol. Biol. 25 (1995) 409-415
14. Takesue S., Yokota K., Miyajima S., Taguchi R., Ikezawa H., and Takesue Y. Partial release of aminopeptidase N from larval midgut cell membranes of the silkworm, Bombyx mori, by phosphatidylinositol-specific phospholipase C. Comp. Biochem. Physiol. B 102 (1992) 7-11
15. Takesue Y., Yokota K., Miyajima K., Taguchi R., and Ikezawa H. Membrane anchors of alkaline phosphatase and trehalase associated with the plasma membrane of larval midgut epithelial cells of the silkworm, Bombyx mori. J. Biochem. 105 (1989) 998-1001
16. Hooper N.M., and Turner A.J. Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl phosphatidylinositol membrane anchor. Biochem. J. 250 (1988) 865-869
17. Jenkins J.L., and Dean D.H. Binding specificity of Bacillus thuringiensis Cry1Aa for purified, native Bombyx mori aminopeptidase N and cadherin-like receptors. BMC Biochem. 2 (2001) 12-19
18. Jenkins J.L., Lee M.K., Valaitis A.P., Curtiss A., and Dean D.H. Bivalent sequential binding model of a Bacillus thuringiensis toxin to gypsy moth aminopeptidase N receptor. J. Biol. Chem. 275 (2000) 14423-14431
19. Knight P.J.K., Crickmore N., and Ellar D.J. The receptor for Bacillus thuringiensis CryIA(c) delta-endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N. Mol. Microbiol. 11 (1994) 429-436
20. Oltean D.I., Pullikuth A.K., Lee H.K., and Gill S.S. Partial purification and characterization of Bacillus thuringiensis Cry1A toxin receptor A from Heliothis virescens and cloning of the corresponding cDNA. Appl. Environ. Microbiol. 65 (1999) 4760-4766
21. Denolf P., Hendrick K., Damme J., Jansens S., Peferoen M., Degheele D., and Van R.J. Cloning and characterization of M. sexta and P. xylostella midgut aminopeptidase N enzymes related to Bacillus thuringiensis toxin-binding proteins. Eur. J. Biochem. 248 (1997) 748-761
22. Luo K., McLachlin J.R., Brown M.R., and Adang M.J. Expression of a glycosylphosphatidylinositol-linked Manduca sexta aminopeptidase N in insect cells. Protein Expr. Purif. 17 (1999) 113-122
23. Simpson R.M., and Newcomb R.D. Binding of Bacillus thuringiensis δ-endotoxins Cry1Ac and Cry1Ba to a 120-kDa aminopeptidase N Epiphyas postvittana purified from both brush border membrane vesicles and baculovirus-infected Sf9 cells. Insect Biochem. Mol. Biol. 30 (2000) 1069-1078
24. Yaoi K., Nakanishi K., Kadotani T., Imamura M., Koizumi N., Iwahana H., and Sato R. cDNA cloning and expression of Bacillus thuringiensis Cry1Aa toxin binding 120-kDa aminopeptidase N from Bombyx mori. Biochim. Biophys. Acta 1444 (1999) 131-137
25. Nakanishi K., Yaoi K., Shimada N., Kadotani T., and Sato R. Bacillus thuringiensis insecticidal Cry1Aa toxin binds to a highly conserved region of aminopeptidaseN in the host insect leading to its evolutionary success. Biochim. Biophys. Acta 1432 (1999) 57-63
26. Schoenle E.J., Adams L.D., and Sammons D.W. Insulin-induced rapid decrease of a major protein in fat cell plasma membrane. J. Biol. Chem. 259 (1984) 12112
27. Hardy F., Ohaniance L.D., and Goldberg M.E. Measurement of antibody/antigen association rate constants in solution by a method based on enzyme-linked immunosorbent assay. J. Immunol. Methods 200 (1997) 155-159
28. Lee M.K., Rajamohan F., Jenkins J.L., Curtiss A.S., and Dean D.H. Role of two arginine residues in domain II, loop 2 of Cry1Ab and Cry1Ac Bacillus thuringiensis δ-endotoxin in toxicity and binding to Manduca sexta and Lymantria dispar aminopeptidase N. Mol. Microbiol. 38 (2000) 289-298
29. Luo K., Sangadala S., Masson L., Mazza A., Rousseau R., and Adang M.J. The Heliothis virescens 170 kDa aminopeptidase functions as receptor A by mediating specific Bacillus thuringiensis Cry1A δ-endotoxin binding and pore formation. Insect Biochem. Mol. Biol. 27 (1997) 735-743
30. Bernard D.G., Jacqueline K., Emmanuel S., Hans N., and Hubert L. Determinants essential for the transmissible gastroenteritis virus-receptor interaction reside within a domain of aminopeptidase N that is distinct from the enzymatic site. J. Virol. 68 (1994) 5216-5224
31. Luo K., and Adang M.J. A 106 kDa form of aminopeptidase is a receptor for Bacillus thuringiensis Cry1C δ-endotoxin in the brush border membrane of Manduca sexta. Insect Biochem. Mol. Biol. 26 (1996) 783-791
32. Gill M., and Ellar D. Transgenic Drosophila reveals a functional in vivo receptor for the Bacillus thuringiensis toxin Cry1Ac1. Insect Mol. Biol. 11 (2002) 619-625
33. Barrows B.D., Griffitts J.S., and Aroian R.V. Caenorhabditis elegans carbohydrates in bacterial toxin resistance. Methods Enzymol. 417 (2006) 340-358
34. Bravo A., Gomez I., Conde J., Munoz-Garay C., Sanchez J., Miranda R., Zhuang M., Gill S.S., and Soberon M. Oligomerization triggers binding of a Bacillus thuringiensis Cry1Ab pore-forming toxin to aminopeptidase N receptor leading to insertion into membrane microdomains. Biochim. Biophys. Acta 1667 1 (2004) 38-46
35. Xuebin Z., Mehmet C., Natalya B.G., Ronald T., and Bulla L.A.J. A mechanism of cell death involving an adenylyl cyclase/PKA signaling pathway is induced by the Cry1Ab toxin Bacillus thuringiensis. Proc. Natl. Acad. Sci. USA 103 (2006) 9897-9902