Strong coupling effects during X-pulse CPMG experiments recorded on heteronuclear ABX spin systems: Artifacts and a simple solution

Journal of Biomolecular NMR

Volumn 38, Issue 1, 2007, Pages 41-46

  Vallurupalli, Pramodh ^a   Scott, Lincoln ^b   Williamson, James R ^b   Kay, Lewis E ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

1H CPMG relaxation dispersion; ABX heteronuclear spin system; RNA; Spin lock; Strong scalar coupling

Indexed keywords

CARBON 13; PROTON; RIBOSE; VIRUS RNA;

ARTICLE; ARTIFACT; CROSS COUPLING REACTION; GENE STRUCTURE; HUMAN IMMUNODEFICIENCY VIRUS 2; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT; QUALITATIVE ANALYSIS; SIMULATION; STRUCTURE ANALYSIS;

MOLECULAR STRUCTURE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; REPRODUCIBILITY OF RESULTS; RNA;

HUMAN IMMUNODEFICIENCY VIRUS 2;

EID: 34248590990     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-006-9139-1     Document Type: Article

References (18)

1. Bezsonova I, Korzhnev DM, Prosser RS, Forman-Kay JD, Kay LE (2006) Hydration and packing along the folding pathway of SH3 domains by pressure-dependent NMR. Biochemistry 45:4711-4719
2. Brodsky AS, Williamson JR (1997) Solution structure of the HIV-2 TAR-argininamide complex. J Mol Biol 267:624-639
3. 13C NMR relaxation. J Mol Biol 317:263-278
4. Dittmer J, Bodenhausen G (2004) Evidence for slow motion in proteins by multiple refocusing of heteronuclear nitrogen/proton multiple quantum coherences in NMR. J Am Chem Soc 126:1314-1315
5. Eisenmesser EZ, Millet O, Labeikovsky W, Korzhnev DM, Wolf-Watz M, Bosco DA, Skalicky JJ, Kay LE, Kern D (2005) Intrinsic dynamics of an enzyme underlies catalysis. Nature 438:117-121
6. 13C NMR spin relaxation. J Am Chem Soc 122:11610-11619
7. Ishima R, Torchia DA (2003) Extending the range of amide proton relaxation dispersion experiments in proteins using a constant-time relaxation-compensated CPMG approach. J Biomol NMR 25:243-248
8. 15N transverse relaxation to detect millisecond time-scale motions in perdeuterated proteins: Application to HIV-1 protease. J Am Chem Soc 120:10534-10542
9. Korzhnev DM, Neudecker P, Mittermaier A, Orekhov VY, Kay LE (2005) Multiplesite exchange in proteins studied with a suite of six NMR relaxation dispersion experiments: An application to the folding of a Fyn SH3 domain mutant. J Am Chem Soc 127:15602-15611
10. Korzhnev DM, Salvatella X, Vendruscolo M, Di Nardo AA, Davidson AR, Dobson CM, Kay LE (2004) Low-populated folding intermediates of Fyn SH3 characterized by relaxation dispersion NMR. Nature 430:586-590
11. Loria JP, Rance M, Palmer AG (1999) A relaxation-compensated Carr-Purcell-Meiboom-Gill sequence for characterizing chemical exchange by NMR spectroscopy. J Am Chem Soc 121:2331-2332
12. 15N relaxation dispersion NMR spectroscopy: Application to Asn and Gln residues in a cavity mutant of T4 lysozyme. J Am Chem Soc 123:967-975
13. Orekhov VY, Korzhnev DM, Kay LE (2004) Double- and zero-quantum NMR relaxation dispersion experiments sampling millisecond time scale dynamics in proteins. J Am Chem Soc 126:1886-1891
14. Palmer AG 3rd (2004) NMR characterization of the dynamics of biomacromolecules. Chem Rev 104:3623-3640
15. 13C-labeled ribonucleotides. Method Enzymol 317:18-38
16. Skrynnikov NR, Mulder FA, Hon B, Dahlquist FW, Kay LE (2001) Probing slow time scale dynamics at methyl-containing side chains in proteins by relaxation dispersion NMR measurements: Application to methionine residues in a cavity mutant of T4 lysozyme. J Am Chem Soc 123:4556-4566
17. Tollinger M, Skrynnikov NR, Mulder FA, Forman-Kay JD, Kay LE (2001) Slow dynamics in folded and unfolded states of an SH3 domain. J Am Chem Soc 123:11341-11352
18. Vallurupalli P, Scott L, Hennig M, Williamson JR, Kay LE (2006) New RNA labeling methods offer dramatic sensitivity enhancements in 2H NMR relaxation spectra. J Am Chem Soc 128:9346-9347