메뉴 건너뛰기




Volumn 630, Issue 1-2, 2007, Pages 122-128

Improved alkaline comet assay protocol for adherent HaCaT keratinocytes to study UVA-induced DNA damage

Author keywords

Adherent HaCaT keratinocytes; Alkaline comet assay; Dose dependence of DNA damage induction; Trypsin effect

Indexed keywords

DNA;

EID: 34248582259     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2007.02.009     Document Type: Article
Times cited : (13)

References (29)
  • 2
    • 0021193203 scopus 로고
    • Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells
    • Ostling O., and Johanson K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123 (1984) 291-298
    • (1984) Biochem. Biophys. Res. Commun. , vol.123 , pp. 291-298
    • Ostling, O.1    Johanson, K.J.2
  • 3
    • 0023919963 scopus 로고
    • A simple technique for quantitation of low levels of DNA damage in individual cells
    • Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988) 184-191
    • (1988) Exp. Cell Res. , vol.175 , pp. 184-191
    • Singh, N.P.1    McCoy, M.T.2    Tice, R.R.3    Schneider, E.L.4
  • 4
    • 0037187201 scopus 로고    scopus 로고
    • DNA repair capacity after gamma-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes
    • Mayer C., Popanda O., Zelezny O., von Brevern M.C., Bach A., Bartsch H., and Schmezer P. DNA repair capacity after gamma-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes. DNA Repair (Amst.) 1 (2002) 237-250
    • (2002) DNA Repair (Amst.) , vol.1 , pp. 237-250
    • Mayer, C.1    Popanda, O.2    Zelezny, O.3    von Brevern, M.C.4    Bach, A.5    Bartsch, H.6    Schmezer, P.7
  • 6
    • 1642354180 scopus 로고    scopus 로고
    • Optimisation of the comet genotoxicity assay in freshly isolated murine hepatocytes: detection of strong in vitro DNA damaging properties for styrene
    • Fontaine F.R., DeGraaf Y.C., Ghaoui R., Sallustio B.C., Edwards J., and Burcham P.C. Optimisation of the comet genotoxicity assay in freshly isolated murine hepatocytes: detection of strong in vitro DNA damaging properties for styrene. Toxicol. In Vitro 18 (2004) 343-350
    • (2004) Toxicol. In Vitro , vol.18 , pp. 343-350
    • Fontaine, F.R.1    DeGraaf, Y.C.2    Ghaoui, R.3    Sallustio, B.C.4    Edwards, J.5    Burcham, P.C.6
  • 8
    • 0025728809 scopus 로고
    • Irradiation of cells attached or suspended by rubber policeman or by trypsin influences the extent of DNA strand breaks induced by ionizing radiation
    • Hill H.Z., and Hill G.J. Irradiation of cells attached or suspended by rubber policeman or by trypsin influences the extent of DNA strand breaks induced by ionizing radiation. Radiat. Res. 125 (1991) 343-345
    • (1991) Radiat. Res. , vol.125 , pp. 343-345
    • Hill, H.Z.1    Hill, G.J.2
  • 10
    • 0025017148 scopus 로고
    • Effects of trypsin on X-ray-induced cell killing, chromosome abnormalities and kinetics of DNA repair in mammalian cells
    • Sprunt E.A., and Bryant P.E. Effects of trypsin on X-ray-induced cell killing, chromosome abnormalities and kinetics of DNA repair in mammalian cells. Mutat. Res. 228 (1990) 211-219
    • (1990) Mutat. Res. , vol.228 , pp. 211-219
    • Sprunt, E.A.1    Bryant, P.E.2
  • 12
    • 0029923890 scopus 로고    scopus 로고
    • Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays
    • Alapetite C., Wachter T., Sage E., and Moustacchi E. Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays. Int. J. Radiat. Biol. 69 (1996) 359-369
    • (1996) Int. J. Radiat. Biol. , vol.69 , pp. 359-369
    • Alapetite, C.1    Wachter, T.2    Sage, E.3    Moustacchi, E.4
  • 13
    • 0034455345 scopus 로고    scopus 로고
    • Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes
    • Petersen A.B., Gniadecki R., Vicanova J., Thorn T., and Wulf H.C. Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes. J. Photochem. Photobiol. B 59 (2000) 123-131
    • (2000) J. Photochem. Photobiol. B , vol.59 , pp. 123-131
    • Petersen, A.B.1    Gniadecki, R.2    Vicanova, J.3    Thorn, T.4    Wulf, H.C.5
  • 14
    • 32644441478 scopus 로고    scopus 로고
    • Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation
    • Hazane F., Sauvaigo S., Douki T., Favier A., and Beani J.C. Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation. J. Photochem. Photobiol. B 82 (2006) 214-223
    • (2006) J. Photochem. Photobiol. B , vol.82 , pp. 214-223
    • Hazane, F.1    Sauvaigo, S.2    Douki, T.3    Favier, A.4    Beani, J.C.5
  • 15
    • 8744233919 scopus 로고    scopus 로고
    • After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available
    • Rapp A., and Greulich K.O. After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available. J. Cell Sci. 117 (2004) 4935-4945
    • (2004) J. Cell Sci. , vol.117 , pp. 4935-4945
    • Rapp, A.1    Greulich, K.O.2
  • 16
    • 0025765751 scopus 로고
    • A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides
    • Singh N.P., Tice R.R., Stephens R.E., and Schneider E.L. A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutat. Res. 252 (1991) 289-296
    • (1991) Mutat. Res. , vol.252 , pp. 289-296
    • Singh, N.P.1    Tice, R.R.2    Stephens, R.E.3    Schneider, E.L.4
  • 18
    • 0031945960 scopus 로고    scopus 로고
    • Epidermal differentiation and basement membrane formation by HaCaT cells in surface transplants
    • Breitkreutz D., Schoop V.M., Mirancea N., Baur M., Stark H.J., and Fusenig N.E. Epidermal differentiation and basement membrane formation by HaCaT cells in surface transplants. Eur. J. Cell Biol. 75 (1998) 273-286
    • (1998) Eur. J. Cell Biol. , vol.75 , pp. 273-286
    • Breitkreutz, D.1    Schoop, V.M.2    Mirancea, N.3    Baur, M.4    Stark, H.J.5    Fusenig, N.E.6
  • 19
    • 0041842714 scopus 로고    scopus 로고
    • Constitutive overexpression of human telomerase reverse transcriptase but not c-myc blocks terminal differentiation in human HaCaT skin keratinocytes
    • Cerezo A., Stark H.J., Moshir S., and Boukamp P. Constitutive overexpression of human telomerase reverse transcriptase but not c-myc blocks terminal differentiation in human HaCaT skin keratinocytes. J. Invest. Dermatol. 121 (2003) 110-119
    • (2003) J. Invest. Dermatol. , vol.121 , pp. 110-119
    • Cerezo, A.1    Stark, H.J.2    Moshir, S.3    Boukamp, P.4
  • 20
    • 0025270234 scopus 로고
    • Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay
    • Olive P.L., Banath J.P., and Durand R.E. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay. Radiat. Res. 122 (1990) 86-94
    • (1990) Radiat. Res. , vol.122 , pp. 86-94
    • Olive, P.L.1    Banath, J.P.2    Durand, R.E.3
  • 21
    • 34248520563 scopus 로고    scopus 로고
    • Federal Office for Radiation Protection, Munich, Germany; http://www.bfs.de. Continuous Solar UV-Monitoring in Germany, June 20, 2006. Ref Type: Report.
  • 22
    • 0037529559 scopus 로고
    • An improved method for the isolation and quantitative cultivation of embryonic cells
    • Rinaldini L.M. An improved method for the isolation and quantitative cultivation of embryonic cells. Exp. Cell Res. 16 (1959) 477-505
    • (1959) Exp. Cell Res. , vol.16 , pp. 477-505
    • Rinaldini, L.M.1
  • 23
    • 0024496396 scopus 로고
    • Trypsinization and the radiosensitivity of mitotic and log phase Chinese hamster V79 cells exposed to 250 kVp X-rays
    • Reddy N.M., Stevenson A.F., and Lange C.S. Trypsinization and the radiosensitivity of mitotic and log phase Chinese hamster V79 cells exposed to 250 kVp X-rays. Int. J. Radiat. Biol. 55 (1989) 105-117
    • (1989) Int. J. Radiat. Biol. , vol.55 , pp. 105-117
    • Reddy, N.M.1    Stevenson, A.F.2    Lange, C.S.3
  • 24
    • 0025742795 scopus 로고
    • Serum, trypsin, and cell shape but not cell-to-cell contact influence the X-ray sensitivity of Chinese hamster V79 cells in monolayers and in spheroids
    • Reddy N.M., and Lange C.S. Serum, trypsin, and cell shape but not cell-to-cell contact influence the X-ray sensitivity of Chinese hamster V79 cells in monolayers and in spheroids. Radiat. Res. 127 (1991) 30-35
    • (1991) Radiat. Res. , vol.127 , pp. 30-35
    • Reddy, N.M.1    Lange, C.S.2
  • 25
    • 0023183777 scopus 로고
    • Formation of DNA strand breaks in UV-irradiated human fibroblast preparations
    • Kaufmann W.K., and Briley L.P. Formation of DNA strand breaks in UV-irradiated human fibroblast preparations. Mutat. Res. 184 (1987) 47-55
    • (1987) Mutat. Res. , vol.184 , pp. 47-55
    • Kaufmann, W.K.1    Briley, L.P.2
  • 26
    • 0031011221 scopus 로고    scopus 로고
    • The genetic toxicity of time: importance of DNA-unwinding time to the outcome of single-cell gel electrophoresis assays
    • Yendle J.E., Tinwell H., Elliott B.M., and Ashby J. The genetic toxicity of time: importance of DNA-unwinding time to the outcome of single-cell gel electrophoresis assays. Mutat. Res. 375 (1997) 125-136
    • (1997) Mutat. Res. , vol.375 , pp. 125-136
    • Yendle, J.E.1    Tinwell, H.2    Elliott, B.M.3    Ashby, J.4
  • 27
    • 0031776788 scopus 로고    scopus 로고
    • Kinetics of DNA strand breaks and protection by antioxidants in UVA- or UVB-irradiated HaCaT keratinocytes using the single cell gel electrophoresis assay
    • Lehmann J., Pollet D., Peker S., Steinkraus V., and Hoppe U. Kinetics of DNA strand breaks and protection by antioxidants in UVA- or UVB-irradiated HaCaT keratinocytes using the single cell gel electrophoresis assay. Mutat. Res. 407 (1998) 97-108
    • (1998) Mutat. Res. , vol.407 , pp. 97-108
    • Lehmann, J.1    Pollet, D.2    Peker, S.3    Steinkraus, V.4    Hoppe, U.5
  • 28
    • 33746266759 scopus 로고    scopus 로고
    • Effects of hypoxia and limited diffusion in tumor cell microenvironment on bystander effect of P450 prodrug therapy
    • Gunther M., Waxman D.J., Wagner E., and Ogris M. Effects of hypoxia and limited diffusion in tumor cell microenvironment on bystander effect of P450 prodrug therapy. Cancer Gene Ther. 13 (2006) 771-779
    • (2006) Cancer Gene Ther. , vol.13 , pp. 771-779
    • Gunther, M.1    Waxman, D.J.2    Wagner, E.3    Ogris, M.4
  • 29
    • 0034662615 scopus 로고    scopus 로고
    • Diminished DNA repair and elevated mutagenesis in mammalian cells exposed to hypoxia and low pH
    • Yuan J., Narayanan L., Rockwell S., and Glazer P.M. Diminished DNA repair and elevated mutagenesis in mammalian cells exposed to hypoxia and low pH. Cancer Res. 60 (2000) 4372-4376
    • (2000) Cancer Res. , vol.60 , pp. 4372-4376
    • Yuan, J.1    Narayanan, L.2    Rockwell, S.3    Glazer, P.M.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.