Identification of Glu-540 as the catalytic nucleophile of human β- glucuronidase using electrospray mass spectrometry

Journal of Biological Chemistry

Volumn 273, Issue 51, 1998, Pages 34057-34062

  Wong, Alexander W ^a   He, Shouming ^a   Grubb, Jeffrey H ^b   Sly, William S ^b   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLUCURONIDASE; GLUTAMINE;

AMINO ACID ANALYSIS; ARTICLE; CATALYSIS; COVALENT BOND; DERIVATIZATION; ENZYME ACTIVE SITE; ENZYME INACTIVATION; EXTRACELLULAR MATRIX; GLYCOSYLATION; HYDROLYSIS; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL;

AFFINITY LABELS; AMINO ACID SEQUENCE; ANIMALS; CATALYTIC DOMAIN; CONSERVED SEQUENCE; GLUCOSIDES; GLUCURONIDASE; GLUTAMIC ACID; HUMANS; HYDROLYSIS; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MICE; MODELS, CHEMICAL; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; RATS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032545380     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.51.34057     Document Type: Article

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