Detection of methicillin resistance in Staphylococcus aureus isolates using two-step triplex PCR and conventional methods

Journal of Microbiology and Biotechnology

Volumn 17, Issue 4, 2007, Pages 673-676

  Cho, Joon Il ^a   Jung, Hye Jin ^a   Kim, Young Joon ^a   Park, Sung Hee ^a   Ha, Sang Do ^a   Kim, Keun Sung ^a  

^a Chung Ang University   (South Korea)

Author keywords

FemA gene; MecA gene; Methicillin resistant S. aureus (MRSA); Muc gene; Two step triplex PCR

Indexed keywords

BACTERIAL PROTEIN; OXACILLIN; PENICILLIN BINDING PROTEIN 2A; PROTEIN FEMA; PROTEIN NUC; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; FOOD CONTAMINATION; GENE AMPLIFICATION; LATEX AGGLUTINATION TEST; NONHUMAN; PENICILLIN RESISTANCE; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; ENDONUCLEASES; METHICILLIN RESISTANCE; MICROCOCCAL NUCLEASE; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 34248367003     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Barry, A. L. and R. N. Jones. 1987. Reliability of high-content disks and modified broth dilution tests for detecting staphylococcal resistance to the penicillinase-resistant penicillins. J. Clin. Microbiol. 25: 1897-1901.
2. Barski, P., L. Piechowicz, J. Galinski, and J. Kur. 1996. Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR. Mol. Cell. Probes 10: 471-475.
3. Bej, A. K., M. H. Mahbubani, R. Miller, J. L. Dicesare, L. Haff, and R. M. Atlas. 1990. Multiplex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water. Mol. Cell. Probes 4: 353-365.
4. Berger-Bachi, B., L. Barberis-Maino, A. Strassle, and F. Kayser. 1989. FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: Molecular cloning and characterization. Mol. Gen. Genet. 219: 263-269.
5. Bignardi, G E., N. Woodford, A. Chapman, A. P. Johnson, and D. C. Speller. 1996. Detection of the mec-A gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low-level methicillin resistance. J. Antimicrob. Chemother. 37: 53-63.
6. Brakstad, O. G., K. Aasbakk, and J. A. Maeland. 1992. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbiol. 30: 1654-1660.
7. Chambers, H. F. 1988. Methicillin-resistant staphylococci. Clin. Microbiol. Rev. 1: 173-186.
8. Chamers, H. F. 1997. Methicillin resistance staphylococci: Molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10: 781-791.
9. Del Vecchio, V G., J. M. Petroziello, M. J. Gress, F. K. McCeskey, G. P. Melcher, H. K. Crouch, and J. R. Lupski. 1995. Molecular genotyping of methicillin-resistant Staphylococcus aureus via fluorophore-enhanced repetitive-sequence PCR. J. Clin. Microbiol. 33: 2141-2144.
10. Fang H. and G. Hedin. 2003. Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J. Clin. Microbiol. 41: 2894-2899.
11. Geha, D. J., J. R. Uhl, C. A. Gustaferro, and D. H. Persing. 1994. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J. Clin. Microbiol. 32: 1768-1772.
12. Hussain, Z., L. Stoakes, S. Garrow, S. Longo, V. Fitzgerald, and R. Lannigan. 2000. Rapid detection of mecA-positive and mecA-negative coagulase-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test. J. Clin. Microbiol. 38: 2051-2054.
13. Kim, K. S., H. A. Seo, C. Y. Oh, H. Kim, and J. B. Kim. 2001. Comparison of different PCR-based genotyping techniques for MRSA discrimination among methicillin-resistant Staphylococcus aureus isolates. J. Microbiol. Biotechnol. 11: 788-797.
14. Kim, Y. J. 2002. Intraspecies genotyping of Staphylococcus aureus isolates from kimbap using three repetitive DNA sequence PCR techniques. Thesis, Chung-Ang University, Seoul, Korea.
15. Ko, K. S., J. Y. Lee, J. H. Song, J. Y Baek, W. S. Oh, J. Chun, and H. S. Yoon. 2006. Screening of essential genes in Staphylococcus aureus N315 using comparative genomics and allelic replacement mutagenesis. J. Microbiol. Biotechnol. 16: 623-632.
16. Kolbert, C. P., J. Arruda, P. Varga-Delmore, X. Zheng, M., Lewis, J. Kolberg, and D. H. Persing. 1998. Branched-DNA assay for detection of the mecA gene in oxacillin-resistant and oxacillin-sensitive staphylococci. J. Clin. Microbiol. 36: 2640-2644.
17. Lachica, R. V., C. Genigeorgis, and P. D. Hoeprich. 1971. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 21: 585-587.
18. Lem, P., J. Spiegelman, B. Toye, and K. Ramotar. 2001. Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles. Diagn. Microbiol. Infect. Dis. 41: 165-168.
19. Louie, L., S. O. Matsumura, E. Choi, M. Louie, and A. E. Simor. 2000. Evaluation of three rapid methods for detection of methicillin resistance in Staphylococcus aureus. J. Clin. Microbiol. 38: 2170-2173.
20. Maes, N., J. Magdalena, S. Rottiers, Y. De Gheldre, and M. J. Struelens. 2002. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures. J. Clin. Microbiol. 40: 1514-1517.
21. Nunes, E. L., K. R. dos Santos, P. J. Mondino, M. D. Bastos, and M. Giambiagi-de Marval. 1999. Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR. Diagn. Microbiol. Infect. Dis. 34: 77-81.
22. Perez-Roth, E., F. Claverie-Martin, J. Villar, and S. Mendez-Alvarez. 2001. Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J. Clin. Microbiol. 39: 4037-4041.
23. Park, S. H., H. J. Kim, and H. Y. Kim. 2006. Simultaneous detection of Yersinia enterocolitica, Staphylococcus aureus, and Shigella spp. in lettuce using multiplex PCR method. J. Microbiol. Biotechnol. 16: 1301-1305.
24. Ryffel, C., W. Tesch, I. Birch-Machin, P. E. Reynolds, L. Barberis-Maino, F. H. Kayser, and B. Berger-Bachi. 1990. Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Gene 94: 137-138.
25. Salisbury, S. M., L. M Sabatini, and C. A. Spiegel. 1997. Identification of methicillin-resistant staphylococci by multiplex polymerase chain reaction assay. Am. J. Clin. Pathol. 107: 368-373.
26. Schmitz, F. J., C. R. Mackenzie, B. Hofmann, J. Verhoef, M. Finken-Eigen, H. P. Heinz, and K. Kohrer. 1997. Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by multiplex PCR. J. Med. Microbiol. 46: 773-778.
27. Tomasz, A., S. Nachman, and H. Leaf. 1991. Stable classes of phenotypic expression in methicillin-resistant clinical isolates of staphylococci. Antimicrob. Agents Chemother. 35: 124-129.
28. Unal, S., J. Hoskins, J. E. Flokowitsch, C. Y. Wu, D. A. Preston, and P. L. Skatrud. 1992. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J. Clin. Microbiol. 30: 1685-1691.
29. Vannuffel, P., J. Gigi, H. Essedine, B. Vandercam, M. Delmee, G. Wauters, and J. L. Gala. 1995. Specific detection of methicillin-resistant Staphylococcus aureus species by multiplex PCR. J. Clin. Microbiol. 33: 2864-2867.