Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR

Journal of Medical Microbiology

Volumn 46, Issue 9, 1997, Pages 773-778

  Schmitz, F J ^a,c   Mackenzie, C R ^a   Hofmann, B ^a   Verhoef, J ^c   Finken Eigen, M ^b   Heinz, H P ^a   Kohrer K ^b  

^a Inst F Med Mikrobiol/Virologie   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIUM IDENTIFICATION; COAGULASE NEGATIVE STAPHYLOCOCCUS; HUMAN; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PATHOGENICITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INFECTION; TAXONOMY;

EID: 0030883235     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/00222615-46-9-773     Document Type: Article

References (43)

1. Visser MR, Fluit AC. Amplification methods for the detection of bacterial resistance genes. JMIMDQ 1995; 23: 105-116.
2. Mulligan ME, Murray KA, Ripner BS et al. Methicillin resistant Slaphylococcus aureus: a consensus review of the microbiology, pathogenesis, and epidemiology with implications for prevention and management. Am J Med 1993; 94: 313-328.
3. Emori TG, Gaynes RP. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev 1993; 6: 428-442.
4. Hartman BJ, Tomasz A. Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus. Antimicrob Agents Chemother 1986; 29: 85-92.
5. Madiraju MVVS, Brunner DP, Wilkinson BJ. Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in methicillin-resistant Slaphylococcus aureus. Antimicrob Agents Chemother 1987; 31: 1727-1733.
6. Watanakunakorn C. Effect of inoculum size on in vitro susceptibility of methicillin-resistant Staphylococcus aureus to eighteen antimicrobial agents. Eur J Clin Microbiol 1985; 4: 68-70.
7. Suzuki E, Hiramatsu K, Yokota T. Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution. Antimicrob Agents Chemother 1992; 36: 429-434.
8. Archer GL, Pennell E. Detection of methicillin resistance in staphylococci by using a UNA probe. Antimicrob Agents Chemother 1990; 34: 1720-1724.
9. Ligozzi M, Rossolini GM, Tonin EA, Fontana R. Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 1991; 35: 575-578.
10. Suzuki E, Kuwuhara-Arai K, Richardson JF, Iliramatsu K. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrob Agents Chemother 1993; 37: 1219-1226.
11. Maidhof H, Reinicke B, Blümel P, Berger-Bächi B, Labischinski H. femA, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan on methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains. J Bacteriol 1991; 173: 3507-3513.
12. Ryffel C, Kayser FH, Berger-Bächi B. Correlation between regulation of mecA transcription and expression of methicillin resistance in staphylococci. Antimicrob Agents Chemother 1992; 36: 25-31.
13. Ryffel C, Tesch W, Birch-Machin I et al. Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Gene 1990; 94: 137-138.
14. Murakami K, Minamide W, Wada K, Nakamura E, Teraoka H, Watanabe S. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbial 1991; 29: 2240-2244.
15. Predari SC, Ligozzi M, Fontana R, Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction. Antimicrob Agents Chemother 1991; 35: 2568-2573.
16. Ünal S, Hoskins J, Flokowitsch E, Wu CYE, Preston DA, Skatrud PL. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J Clin Microbiol 1992; 30: 1685-1691.
17. Tokue Y, Shoji S, Satoh K, Watanabe A, Motomiya M. Comparison of a polymerase chain reaction assay and a conventional microbiologic method for detection of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1992; 36: 6-9.
18. Ubukata K, Nakagami S, Nitta A et al. Rapid identification of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products. J Clin Microbiol 1992; 30: 1728-1733.
19. Hiramatsu K, Suzuki E, Takayama Y, Katayama Y, Yokota T. Role of penicillinase plasmids in the stability of the mecA gene in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1990; 34: 600-604.
20. Hedin G, Löfdahl S. Detecting methicillin-resistant Staphylococcus epidermidis - disc diffusion, broth breakpoint or polymerase chain reaction? APMIS 1993; 101: 311-318.
21. Phonimdaeng P, O'Reilly M, Nowlan P, Bramley AJ, Foster TJ. The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Mol Microbiol 1990; 4: 393-404.
22. Zambardi G, Reverdy ME, Bland S, Bes M, Freney J, Fleurette J. Laboratory diagnosis of oxacillin resistance in Staphylococcus aureus by a multiplex-polymerase chain reaction assay. Diagn Microbiol Infect Dis 1994; 19: 25-31.
23. Brakstad OG, Tveten Y, Nato F, Fournier JM. Comparison of various methods and reagents for specific identification of Staphylococcus aureus positive or negative for the mecA gene. APMIS 1993; 101: 651-654.
24. Vannuffel P, Gigi J, Ezzedine H et al. Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol 1995; 33: 2864-2867.
25. Geha DJ, Uhl JR, Gustaferro CA, Persing DH. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J Clin Microbiol 1994; 32: 1768-1772.
26. Schmitz F-J, Kitzrow M, Haupt C et al. Antimicrobial susceptibility and typing of eoagulase-negative staphylococci from different intensive care units. Clin Lab 1996; 42: 403-410.
27. Schmitz F-J, Geisel R, Wagner S et al. Typing antimicrobial susceptibility and frequency of methicillin-resistant Staphylacoccus aureus strains on a surgical intensive care unit (ICU). Zentral Hyg Umweltmed 1996; 198: 355-380.
28. Deutsches Institut für Normung: Empfindlichkeitsprüfung von Krankheitserregern gegen Chemotherapeutika. In: DIN-Taschenbuch Medizinische Mikrobiologie und Immunologic. Berlin, Beuth. 1992: 331-406.
29. Ryffel C, Tesch W, Birch-Machin I et al. Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermisis, bases 1-2322, Eintrag in die Genbank 1990.
30. Greisen K, Loeffelholz M, Purohit A, Leong D. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol 1994; 32: 335-351.
31. Leong DU. Staphylococcus aureus ATCC 25923 16S rRNA gene, partial sequence, bases 1-366. Eintrag in die Genbank 1993.
32. De Lencastre HM, Sá Figueiredo AM, Urban C, Rahal J, Tomasz A. Multiple mechanisms of methicillin-resistance and improved methods for detection in clinical isolates of Staphylococcus aureus. Antimicrob Agents Chemother 1991; 35: 632-639.
33. Hackbart C, Chambers H. Methicillin-resistant staphylococci: detection methods and treatment of infections. Antimicrob Agents Chemother 1989; 33: 995-999.
34. DeLencastre HM, Tomasz A. Reassessment of the number of auxiliary genes essential for expression of high level methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 1994; 38: 2590-2598.
35. National Committee for Clinical Laboratory Standards. Approved standard M7-A2. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 2nd edn. Villanova, PA, National Committee for Clinical Laboratory Standards. 1990.
36. National Committee for Clinical Laboratory Standards. Approved standard M2-A4. Antimicrobial disk susceptibility tests, 4th edn. Villanova, PA, National Committee for Clinical Laboratory Standards. 1990.
37. Gerberding JL, Muck C, Liu HH, Chambers HF. Comparison of conventional susceptibility tests with direct detection of penicillin-binding protein 2a in borderline oxacillin-resistant strains of Staphylococcus aureus. Antimicrob Agents Chemother 1991; 35: 2574-2579.
38. Massiddia O, Montanari MP, Varaldo PE. Evidence for methicillin-hydrolyzing β-lactamase in Staphylococcus aureus strains with borderline susceptibility to this drug. FEMS Lett 1992; 92: 223-228.
39. Montanari MP, Tonin E, Biavasco F, Varaldo PE, Further characterization of borderline methicillin-resistant Staphylocnccus aureus and analysis of penicillin-binding proteins. Antimicrob Agents Chemother 1990; 34: 911-913.
40. Murakami K, Nomura K, Doi M, Yoshida T. Production of low-affinity penicillin-binding protein by low- and high-resistance groups of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1987; 31: 1307-1311.
41. McDougal LK, Thornsberry C. The role of β-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins. J Clin Microbiol 1986; 23: 832-839.
42. Tomasz A, Drugeon HB, DeLencastre HM, Jabes D, McDougall L, Bille J. New mechanism for methicillin-resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding protein in methicillin-resistant staphylococci. Antimicrob Agents Chemother 1989; 33: 1869-1874.
43. Pfaller MA, Herwaldt LA. Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clin Microbiol Rev 1988; 1: 281-299.