Role of α-Asp181, β-Asp192, and γ-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase

Biochemistry

Volumn 46, Issue 18, 2007, Pages 5391-5397

  Bzymek, Krzysztof P ^a   Colman, Roberta F ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CATALYSIS; METAL IONS; PURIFICATION;

ADENOSIN TRIPHOSPHATE (ADP); ISOCITRATE DEHYDROGENASE;

COENZYMES;

ADENOSINE DIPHOSPHATE; ASPARAGINE; ASPARTIC ACID; ASPARTIC ACID DERIVATIVE; ISOASPARTIC ACID; ISOCITRATE DEHYDROGENASE; ISOCITRIC ACID; MANGANESE; METAL ION; NICOTINAMIDE ADENINE DINUCLEOTIDE; ISOCITRATE DEHYDROGENASE (NADP+); PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; COENZYME; ENZYME BINDING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBUNIT; MICHAELIS CONSTANT; MUTANT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; CHEMISTRY; ENZYME ACTIVE SITE; GENETICS; HUMAN; METABOLISM; PHYSIOLOGY; STRUCTURE ACTIVITY RELATION;

ADENOSINE DIPHOSPHATE; ASPARAGINE; ASPARTIC ACID; CATALYTIC DOMAIN; HUMANS; ISOCITRATE DEHYDROGENASE; MUTAGENESIS, SITE-DIRECTED; NAD; PROTEIN SUBUNITS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 34248227459     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi700061t     Document Type: Article

References (22)

1. Haselback, R. J., and McAlister-Henn, L. (1993) Function and Expression of Yeast Mitochondrial NAD- and NADP-specific Isocitrate Dehydrogenases, J. Biol. Chem. 268, 12116-12122.
2. Jennings, G. T., Sechi, S., Stevenson, P. M., Tuckey, R. C., Parmelee, D., and McAlister-Henn, L. (1994) Cytosolic NADP-(+)-dependent Isocitrate Dehydrogenase. Isolation of Rat cDNA and Study of Tissue-specific and Developmental Expression of mRNA, J. Biol. Chem. 269, 23128-23134.
3. Ramachandran, N., and Colman, R. F. (1980) Chemical Characterization of Distinct Subunits of Pig Heart DPN- specific Isocitrate Dehydrogenase, J. Biol. Chem. 255, 8859-8864.
4. +-dependent Isocitrate Dehydrogenase β Subunit Isoform, J. Biol. Chem. 274, 36866-36875.
5. Ehrlich, R. S., and Colman, R. F. (1981) Binding of Ligands to Half of Subunits of NAD-dependent Isocitrate Dehydrogenase from Pig Heart. Binding of Manganous Ion, Isocitrate, ADP and NAD, J. Biol. Chem. 256, 1276-1282.
6. Ehrlich, R. S., and Colman, R. F. (1982) Interrelationships among Nucleotide Binding Sites of Pig Heart NAD-dependent Isocitrate Dehydrogenase, J. Biol. Chem. 257, 4769-4774.
7. Ehrlich, R. S., and Colman, R. F. (1983) Separation, Recombination, and Characterization of the DPN-dependent Isocitrate Dehydrogenase from Pig Heart, J. Biol. Chem. 258, 7079-7086.
8. King, M. M., and Colman, R. F. (1983) Affinity Labeling of Nicotinamide Adenine Dinucleotide Dependent Isocitrate Dehydrogenase by the 2′3′-Dialdehyde Derivative of Adenosine 5′-Diphosphate. Evidence for the Formation of an Unusual Reaction Product, Biochemistry 22, 1656-1665.
9. Huang, Y.-C., and Colman, R. F. (1984) Affinity Labeling of the Allosteric ADP Activation Site of NAD-dependent Isocitrate Dehydrogenase by 6-(4-Bromo-2,3-dioxobutyl)thioadenosine 5′-Diphosphate, J. Biol. Chem. 259, 12481-12488.
10. Huang, Y. C., Bailey, J. M., and Colman, R. F. (1986) Inactivation of NAD-dependent Isocitrate Dehydrogenase by Affinity Labeling of the Allosteric ADP Site by 2-(4-Bromo-2,3-dioxobutylthio)-adenosine 5′-Diphosphate, J. Biol. Chem. 261, 14100-14107.
11. +-dependent Isocitrate Dehydrogenase, J. Biol. Chem. 264, 12208-12214.
12. Huang, Y. C., and Colman, R. F. (1990) Subunit Location and Sequences of the Cysteinyl Peptides of Pig Heart NAD-dependent Isocitrate Dehydrogenase, Biochemistry 29, 8266-8273.
13. 2+-binding Aspartates from α, β and γ Subunits of Human NAD-dependent Isocitrate Dehydrogenase. J. Biol. Chem. 281, 21073-21081.
14. Soundar, S., Park, J.-H., Huh, T.-L., and Colman, R. F. (2003) Evaluation by Mutagenesis of the Importance of 3 Arginines in α, β, and γ Subunits of Human NAD-dependent Isocitrate Dehydrogenase, J. Biol. Chem. 278, 52146-52153.
15. Ehrlich, R. S., Hayman, S., Ramachandran, N., and Colman, R. F. (1981) Re-evaluation of Molecular Weight of Pig Heart NAD-specific Isocitrate Dehydrogenase, J. Biol. Chem. 256, 10560-10564.
16. Huang, Y. C., Kumar, A., and Colman, R. F. (1997) Identification of the Subunits and Target Peptides of Pig Heart NAD-specific Isocitrate Dehydrogenase Modified by the Affinity Label 8-(4-Bromo-2,3-dioxobutylthio)NAD, Arch. Biochem. Biophys. 348, 207-218.
17. Colman, R. F. (1983) Aspects of the Structure and Function of the Isocitrate Dehydrogenases, Pept. Protein Rev. 1, 41-69.
18. Hurley, J. H., Dean, A. M., Koshland, D. E., Jr., and Stroud, R. M. (1991) Catalytic Mechanism of NADP-dependent Isocitrate Dehydrogenase: Implications from the Structures of Mg-Isocitrate and NADP Complexes, Biochemistry 30, 8671-8678.
19. 2+ and Isocitrate, J. Biol. Chem. 277, 43454-43462.
20. Hall, M. D., and Banaszak, L. J. (1993) Crystal Structure of a Ternary Complex of Escherichia coli Malate Dehydrogenase, Citrate and NAD at 1.9 Å Resolution, J. Mol. Biol. 232, 213-222.
21. +: Ligand-induced Loop Closing and Mechanism for Cofactor Specificity, Structure 2, 1007-1016.
22. + Specificity-Reversal Mutant, Biochemistry 35, 5670-5678.