Establishment of subcellular fractionation techniques to monitor the intracellular fate of polymer therapeutics II. Identification of endosomal and lysosomal compartments in HepG2 cells combining single-step subcellular fractionation with fluorescent imaging

Journal of Drug Targeting

Volumn 15, Issue 1, 2007, Pages 37-50

  Manunta, Maria ^a   Izzo, Lorella ^a   Duncan, Ruth ^a   Jones, Arwyn Tomos ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Dendrimers; Intracellular trafficking; Polymer therapeutics; Subcellular fractionation

Indexed keywords

BIOLOGICAL MARKER; DEXTRAN; EARLY ENDOSOME ANTIGEN 1; GLUCOSAMINIDASE; HORSERADISH PEROXIDASE; IODIXANOL; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; MACROGOL DERIVATIVE; POLYETHYLENEGLYCOL POLYESTER DENDRON G4; POLYMER; TRANSFERRIN; UNCLASSIFIED DRUG; ACETYLGLUCOSAMINIDASE; COLORING AGENT; LACTATE DEHYDROGENASE; LAMP1 PROTEIN, HUMAN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN; MEMBRANE PROTEIN; POLYESTER; TRYPAN BLUE; VESICULAR TRANSPORT PROTEIN;

ANTIBODY LABELING; ARTICLE; CELL COMPARTMENTALIZATION; CELL DAMAGE; CELL FATE; CELL FRACTIONATION; CELL LYSATE; CELL POPULATION; CELL STRAIN HEPG2; CELL VACUOLE; COLLOID; CONTROLLED STUDY; DENSITY GRADIENT; ENDOCYTOSIS; ENDOSOME; ENZYME ACTIVITY; FLUORESCENCE ANALYSIS; HEPATOMA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; INCUBATION TIME; INTERNALIZATION; LYSOSOME; MACROMOLECULE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; SUPERNATANT; WHOLE CELL; CELL LINE; DENSITY GRADIENT CENTRIFUGATION; DRUG EFFECT; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; GM2 GANGLIOSIDOSIS; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ACETYLGLUCOSAMINIDASE; CELL FRACTIONATION; CELL LINE; CENTRIFUGATION, DENSITY GRADIENT; COLORING AGENTS; DEXTRANS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOCYTOSIS; ENDOSOMES; FLUORESCENT ANTIBODY TECHNIQUE; GANGLIOSIDOSES, GM2; L-LACTATE DEHYDROGENASE; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEINS; LYSOSOMES; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; POLYESTERS; POLYETHYLENE GLYCOLS; POLYMERS; SUBCELLULAR FRACTIONS; TRYPAN BLUE; VESICULAR TRANSPORT PROTEINS;

EID: 34248225726     PISSN: 1061186X     EISSN: 10292330     Source Type: Journal    
DOI: 10.1080/10611860601010330     Document Type: Article

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