Establishment of subcellular fractionation techniques to monitor the intracellular fate of polymer therapeutics I. Differential centrifugation fractionation B16F10 cells and use to study the intracellular fate of HPMA copolymer-doxorubicin

Journal of Drug Targeting

Volumn 14, Issue 6, 2006, Pages 375-390

  Philipp Seib, F ^a,b   Jones, Arwyn T ^a   Duncan, Ruth ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b LEIBNIZ INSTITUTE OF POLYMER RESEARCH DRESDEN   (Germany)

Author keywords

Differential centrifugation; Endocytosis; Marker enzymes; PK1; Polymer therapeutics; Subcellular fractionation

Indexed keywords

ALKALINE PHOSPHATASE; CELL NUCLEUS DNA; COPOLYMER; DOXORUBICIN; LACTATE DEHYDROGENASE; N 2 HYDROXYPROPYLMETHACRYLAMIDE; N ACETYL BETA GLUCOSAMINIDASE; SUCCINATE DEHYDROGENASE; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL CELL; ANIMAL MODEL; ARTICLE; BIOACCUMULATION; CELL ACTIVITY; CELL FRACTIONATION; CELL MEMBRANE; CELL NUCLEUS; CELL ORGANELLE; CELL POPULATION; CELL TRANSPORT; CENTRIFUGATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOSOL; DRUG CONJUGATION; EXPERIMENTAL STUDY; FLUORESCENCE MICROSCOPY; LYSOSOME; MELANOMA B16; MELANOMA CELL; MITOCHONDRION; MONITORING; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; REPRODUCIBILITY; VALIDATION STUDY;

ALKALINE PHOSPHATASE; ANIMALS; CELL FRACTIONATION; CELL LINE, TUMOR; CELL MEMBRANE; CELL NUCLEUS; CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; DOSE-RESPONSE RELATIONSHIP, DRUG; DOXORUBICIN; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; INTRACELLULAR FLUID; L-LACTATE DEHYDROGENASE; LYSOSOMES; MICROSCOPY, CONFOCAL; MICROSOMES; MITOCHONDRIA; PINOCYTOSIS; POLYMETHACRYLIC ACIDS; REPRODUCIBILITY OF RESULTS; SUBCELLULAR FRACTIONS; SUCCINATE DEHYDROGENASE;

EID: 33746998907     PISSN: 1061186X     EISSN: 10292330     Source Type: Journal    
DOI: 10.1080/10611860600833955     Document Type: Article

References (50)

1. Astriab-Fisher, A and Sergueev, D and Fisher, M and Shaw, BR and Juliano, RL. (2002) Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides: Effects on cellular uptake, binding to target sequences, and biologic actions Pharm Res, 19, pp. 744-754.
2. Avruch, J and Hoelzl Wallach, DF. (1971) Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells Biochim Biophys Acta, 233, pp. 334-347.
3. Bachur, NR and Moore, AL and Bernstein, JG and Liu, A. (1970) Tissue distribution and disposition of daunomycin (NSC-82151) in mice: Fluorometric and isotopic methods Cancer Chemother Rep, 54, pp. 89-94.
4. De Duve, C and Pressman, BC and Gianetto, R and Wattiaux, R and Appelmans, F. (1955) Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue Biochem J, 60, pp. 604-617.
5. Duncan, R and Cable, HC and Lloyd, JB and Rejmanova, P and Kopecek, J. (1984) Polymers containing enzymatically degradable bonds, 7. Design of oligopeptide side chains in poly [N-(2-hydroxypropyl)methacrylamide] copolymers to promote efficient degradation by lysosomal enzymes Makromol Chem, 184, pp. 1997-2008.
6. Duncan, R and Seymour, LCW and Scarlett, L and Lloyd, JB and Rejmanova, P and Kopecek, J. (1986) Fate of N-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats Biochim Biophys Acta, 880, pp. 62-71.
7. Duncan, R. (2003a) Polymer - drug conjugates. In Handbook of anticancer drug development. (pp. 239-260). New York: Lippincott Williams & Wilkins.
8. Duncan, R. (2003b) The dawning era of polymer therapeutics Nat Rev Drug Discov, 2, pp. 347-360.
9. Duncan, R. (2005) N-(2-Hydroxypropyl)methacrylamide copolymer conjugates. In Polymeric drug delivery systems. (pp. 1-92). New York: Marcel Dekker.
10. Duncan, R. (2006) Polymer therapeutics as anticancer nanomedicines Nat Rev Cancer submitted.
11. Gianasi, E and Wasil, M and Evagorou, EG and Keddle, A and Wilson, G and Duncan, R. (1999) HPMA copolymer platinates as novel antitumour agents: In vitro properties, pharmacokinetics and antitumour activity in vivo Eur J Cancer, 35, pp. 994-1002.
12. Graham, JM. (1993) The identification of subcellular fractions from mammalian cells. In Methods in molecular biology, biomembrane protocols: I. Isolation and analysis. (pp. 1-18). Totowa: Humana Press Inc. Vol. 19
13. Gruenberg, JE and Howell, KE. (1986) Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system EMBO J, 5(12), pp. 3091-3101.
14. Hovorka, O and St'astny, M and Etrych, T and Subr, V and Srohalm, J and Ulbrich, K and Rihova, B. (2002) Differences in the intracellular fate of free and polymer-bound doxorubicin J Control Release, 80, pp. 101-117.
15. Kapuscinski, J and Skoczylast, B. (1977) Simple and rapid fluorimetric method for DNA microassay Anal Biochem, 83, pp. 252-257.
16. Klemm, AR and Young, D and Lloyd, JB. (1998) Effects of polyethylenimine on endocytosis and lysosomal stability Biochem Pharmacol, 56, pp. 41-46.
17. Knox, WE. (1976) Appendicies I and II. Analyses of rat tissues. In Enzyme pattern in fetal, adult and neoplastic rat tissues 2nd ed. (pp. 254-332). New York: S Krager AG.
18. Kopitz, J and Kisen, GO and Gordon, PB and Bohley, P and Seglen, PO. (1990) Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes J Cell Biol, 111, pp. 941-953.
19. Kovar, M and Kovar, L and Subr, V and Etrych, T and Ulbrich, K and Mrkvan, T and Loucka, J and Rihova, B. (2004) HPMA copolymers containing doxorubicin bound by a proteolytically or hydrolytically cleavable bond: Comparison of biological properties in vitro J Control Release, 99, pp. 301-314.
20. Laurent, N and Wattiaux-De Coninck, S and Mihaylova, E and Leontieva, E and Warnier-Pirotte, MT and Wattiaux, R and Jadot, M. (1999) Uptake by rat liver and intracellular fate of plasmid DNA complexed with poly-l-lysine or poly-D-lysine FEBS Lett, 443, pp. 61-65.
21. Lecocq, M and Wattiaux-De Coninck, S and Laurent, N and Wattiaux, R and Jadot, M. (2000) Uptake and intracellular fate of polyethylenimine in vivo Biochem Biophys Res Commun, 278, pp. 414-418.
22. Lopez-Saura, P and Trouet, A and Tulkens, P. (1978) Analytical fractionation of cultured hepatoma cells (HTC cells) Biochim Biophys Acta, 543, pp. 430-449.
23. Malik, N and Evagorou, EG and Duncan, R. (1999) Dendrimer-platinate: A novel approach to cancer chemotherapy Anticancer Drugs, 10, pp. 767-776.
24. Manunta, M and Izzo, L and Duncan, R and Jones, AT. (2006) Establishment of subcellular fractionation techniques that can be used to monitor the intracellular fate of polymer therapeutics II. A gradient-based fractionation J Drug Target. In press
25. Matsumura, Y and Maeda, H. (1986) A new concept for macromolecular therapeutics in cancer-chemotherapy: Mechanism of tumouritropic accumulation of proteins and antitumour agent SMANCS Cancer Res, 46, pp. 6387-6392.
26. Noel, G and Peterson, C and Trouet, A and Tulkens, P. (1978) Uptake and subcellular localisation of daunorubicin and adriamycin in cultured fibroblasts Eur J Cancer, 14, pp. 363-368.
27. Omelyanenko, V and Kopeckova, P and Gentry, C and Kopecek, J. (1998) Targetable HPMA copolymer-adriamycin conjugates. Recognition, internalization, and subcellular fate J Cont Rel, 53, pp. 25-37.
28. Orlow, SJ. (1995) Melanosomes are specialised members of the lysosomal lineage of organelles J Invest Dermatol, 105, pp. 3-7.
29. Ormerod, MG. (2000) Estimating cell viability. In Flow cytometry. (pp. 249-251). Oxford: Oxford University Press.
30. Rademaker-Lakhai, JM and Terret, C and Howell, SB and Baud, CM and De Boer, RF and Pluim, D and Beijnen, JH and Schellens, JH and Droz, JP. (2004) A phase I and pharmacological study of the platinum polymer AP5280 given as an intravenous infusion once every 3 weeks in patients with solid tumors Clin Cancer Res, 10, pp. 3386-3395.
31. Rejmanova, P and Kopecek, J and Duncan, R and Lloyd, JB. (1985) Stability in rat plasma and serum of lysosomally degradable oligopeptide sequences in N-(2-hydroxypropyl)methacrylamide copolymers Biomaterials, 6, pp. 45-48.
32. Rickwood, D. (1992) Appendix 4: Marker enzymes and chemical assays for the analysis of subcellular fractions. In Preparative centrifugation: A practical approach. (pp. 369-387). Oxford: Oxford University Press.
33. Rozhin, J and Robinson, D and Stevens, MA and Lah, TT and Honn, KV and Ryan, RE and Sloane, BF. (1987) Properties of a plasma membrane-associated cathepsin B-like cysteine proteinase in metastatic B16 melanoma variants Cancer Res, 47, pp. 6620-6628.
34. Rozhin, J and Wade, RL and Honn, KV and Sloane, BF. (1989) Membrane-associated cathepsin L: A role in metastasis of melanomas Biochem Biophys Res Commun, 164, pp. 556-561.
35. Sarapa, N and Britto, MR and Speed, W and Jannuzzo, MG and Breda, M and James, C and Porro, MG and Rocchetti, M and Nygren, P. (2003) Targeted delivery and prefe rential uptake in solid cancer of MAG-CPT, a polymer bound prodrug of camptothecin - a trial in patients undergoing surgery for colorectal carcinoma Cancer Chemother Pharmacol, 52, pp. 424-430.
36. Seiji, M and Shimao, K and Birbeck, MSC and Fitzpatrick, TB. (1963) Subcellular localisation of melanin biosynthesis Ann NY Ac Sci, 100, pp. 497-533.
37. Seymour, LW and Ulbrich, K and Steyger, PS and Brereton, M and Subr, V and Strohalm, J and Duncan, R. (1994) Tumour tropism and anti-cancer efficacy of polymer-based doxorubicin prodrugs in the treatment of subcutaneous murine B16F10 melanoma Br J Cancer, 70, pp. 636-641.
38. Sgouras, D and Duncan, R. (1990) Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1- Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity J Mater Sci Mater Med, 1, pp. 61-68.
39. TM, CT-2103): A macromolecular taxane J Control Release, 109, pp. 120-126.
40. Smith, PK and Krohn, RI and Hermanson, GT and Mallia, AK and Gartner, FH and Provenzano, MD and Fujimoto, EK and Goeke, NM and Olson, BJ and Klenk, DC. (1985) Measurement of protein using bicinchoninic acid Anal Biochem, 150, pp. 76-85.
41. Tijerina, M and Kopeckova, P and Kopecek, J. (2001) The effects of subcellular localisation of N-(2-hyrdoxypropyl)methacrylamide copolymer-Mce6 conjugates in a human ovarian carcinoma J Control Release, 74, pp. 269-273.
42. 6 conjugates with chemotherapeutic activity in human ovarian carcinoma cells Pharm Res, 20, pp. 728-737.
43. Trouet, A and Jolles, G. (1984) Targeting of daunorubicin by association with DNA or proteins: A review Semin Oncol, 11, pp. S64-S72.
44. Tulkens, P and Beaufay, H and Trouet, A. (1974) Analytical fractionation of homogenates from cultured rat embryo fibroblasts J Cell Biol, 63, pp. 383-401.
45. Vasey, PA and Kaye, SB and Morrison, R and Twelves, C and Wilson, P and Duncan, R and Thomson, AH and Murray, LS and Hilditch, TE and Murray, T and Burtles, S and Fraier, D and Frigerio, E and Cassidy, J. (1999) Phase I clinical and pharmacokinetic study of PK1 [N-(2-hydroxypropyl)methacrylamide copolymer doxorubicin]: First member of a new class of chemotherapeutic agents - Drug-polymer conjugates Clin Cancer Res, 5, pp. 83-94.
46. Vassault, A. (1983) Lactate dehydrogenase. In Methods of enzymatic analysis: Enzymes I oxidoreductases, transferases. (pp. 118-126). Basel: Verlag Chemie.
47. Vicent, MJ and Greco, F and Nicholson, RI and Duncan, R. (2005) Polymer-drug conjugates as a novel combination therapy for the treatment of hormone-dependent cancers Angew Chem Int Ed, 44, pp. 2-6.
48. Wedge, SR and Duncan, R and Kopeckova, P. (1991) Comparison of the liver subcellular distribution of free daunomycin and that bound to galactosamine targeted N-(2-hydroxypropyl)methacrylamide copolymers, following intravenous administration in the rat Br J Cancer, 63, pp. 546-549.
49. Zenebergh, A and Schneider, YJ and Loni, M-C and Trouet, A. (1981) Enzymatic characterisation and analytical fraction of L1210 cells Biochimie, 63, pp. 411-418.
50. Zenebergh, A and Baurain, R and Trouet, A. (1984) Cellular pharmacology of detorubicin and doxorubicin in L1210 cells Eur J Cancer Clin Oncol, 20, pp. 115-121.