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The complete ClpB/Hsp100 chaperone family in Arabidopsis is shown to consist of one cytosolic/nuclear member (Hsp101), one mitochondrion-targeted member and one chloroplast-targeted member. A null mutation of the chloroplast-targeted gene is seedling lethal. See also [15].
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Kotak S., Vierling E., Bäumlein H., and von Koskull-Döring P. A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis. Plant Cell 19 (2007) 182-195
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The authors of this paper present the very interesting observation that Hsp21, which is targeted to plastids, can promote plastid differentiation when overexpressed in tomato. Their results also support previous observations supporting a role for Hsp21 (direct or indirect) in protection from oxidative stress.
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Neta-Sharir I., Isaacson T., Lurie S., and Weiss D. Dual role for tomato heat shock protein 21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation. Plant Cell 17 (2005) 1829-1838. The authors of this paper present the very interesting observation that Hsp21, which is targeted to plastids, can promote plastid differentiation when overexpressed in tomato. Their results also support previous observations supporting a role for Hsp21 (direct or indirect) in protection from oxidative stress.
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Nover L., Bharti K., Döring P., Mishra S.K., Ganguli A., and Scharf K.D. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?. Cell Stress Chaperones 6 (2001) 177-189
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Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization
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Kotak S., Port M., Ganguli A., Bicker F., and von Koskull-Döring P. Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization. Plant J 39 (2004) 98-112
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Bharti K., von Koskull-Döring P., Bharti S., Kumar P., Tintschl-Körbitzer A., Treuter E., and Nover L. Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1. Plant Cell 16 (2004) 1521-1535
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Lohmann C., Eggers-Schumacher G., Wunderlich M., and Schöffl F. Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis. Mol Genet Genomics 271 (2004) 11-21
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Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana
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The authors analyzed the consequences of double knockout of HsfA1a and HsfA1b on the whole Arabidopsis transcriptome. For the first time, the essential role of these HSFs in the regulation of HSP and non-conventional HS-responsive genes, such as coding for enzymes involved in sugar metabolism, was shown.
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Busch W., Wunderlich M., and Schöffl F. Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana. Plant J 41 (2005) 1-14. The authors analyzed the consequences of double knockout of HsfA1a and HsfA1b on the whole Arabidopsis transcriptome. For the first time, the essential role of these HSFs in the regulation of HSP and non-conventional HS-responsive genes, such as coding for enzymes involved in sugar metabolism, was shown.
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The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis
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In this study, HsfA2 was analyzed as a dominant HSF under HS conditions in Arabidopsis. Transcriptome analysis of an HsfA2 knockout line under HS conditions revealed its important role for the regulation of subsets of HSP genes. For functional characterization, analysis of HSF-binding sites in target promoters by DNA-binding and transient reporter assays was included.
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Schramm F., Ganguli A., Kiehlmann E., Englich G., Walch D., and von Koskull-Döring P. The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis. Plant Mol Biol 60 (2006) 759-772. In this study, HsfA2 was analyzed as a dominant HSF under HS conditions in Arabidopsis. Transcriptome analysis of an HsfA2 knockout line under HS conditions revealed its important role for the regulation of subsets of HSP genes. For functional characterization, analysis of HSF-binding sites in target promoters by DNA-binding and transient reporter assays was included.
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40
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The authors analyzed T-DNA insertion mutants of 48 HS-induced genes and found that only HsfA2 knockout plants showed a significant HS-sensitive phenotype when the HS treatment was repeated after prolonged recovery phases.
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By analyzing the heat sensitivity of multiple Arabidopsis mutants, this report shows the involvement of several genes other than classical HSP-encoding genes in basal or acquired thermotolerance. Mutant phenotypes also support a possible role of phytohormones, such as ABA, SA and ethylene, and ROS in HS signaling.
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Larkindale J., Hall J.D., Knight M.R., and Vierling E. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol 138 (2005) 882-897. By analyzing the heat sensitivity of multiple Arabidopsis mutants, this report shows the involvement of several genes other than classical HSP-encoding genes in basal or acquired thermotolerance. Mutant phenotypes also support a possible role of phytohormones, such as ABA, SA and ethylene, and ROS in HS signaling.
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This paper describes how the constitutive expression of the stress-response transcriptional coactivator MULTIPROTEIN BRIDGING FACTOR 1C (MBF1c) in Arabidopsis enhances the tolerance of transgenic plants to bacterial infection, heat, and osmotic stress. Transcriptome profiling and inhibitor studies suggest that MBF1c expression enhances tolerance through alterations of the ethylene-response signal transduction pathway, and through altering the production of specific defense-related transcripts.
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