A new transcript splice variant of the human glucocorticoid receptor: Identification and tissue distribution of hGRΔ313-338, an alternative exon 2 transactivation domain isoform

Annals of the New York Academy of Sciences

Volumn 1095, Issue , 2007, Pages 334-341

  Turner, Jonathan D ^a   Schote, Andrea B ^a,b   Keipes, Marc ^c   Muller, Claude P ^a,b  

^a LABORATOIRE NATIONAL DE SANTÉ   (Luxembourg)

^b UNIVERSITY OF TRIER   (Germany)

^c Clinic St Therese   (Luxembourg)

Author keywords

Glucocorticoid receptor; Isoform; Splice variant

Indexed keywords

GLUCOCORTICOID RECEPTOR; ISOPROTEIN; MESSENGER RNA;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; CONFERENCE PAPER; CONTROLLED STUDY; DNA BINDING MOTIF; EXON; GENE DELETION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; RNA ANALYSIS; RNA SPLICING; TRANSACTIVATION; TRANSGENIC MOUSE; ALTERNATIVE RNA SPLICING; ARTICLE; GENETIC VARIABILITY; GENETICS; HELA CELL; METABOLISM; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE; TISSUE DISTRIBUTION; TUMOR CELL LINE;

MUS MUSCULUS;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; BASE SEQUENCE; CELL LINE, TUMOR; EXONS; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN ISOFORMS; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, GLUCOCORTICOID; RNA, MESSENGER; TISSUE DISTRIBUTION; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC; VARIATION (GENETICS);

EID: 34247893813     PISSN: 00778923     EISSN: 17496632     Source Type: Book Series    
DOI: 10.1196/annals.1397.037     Document Type: Conference Paper

References (12)

1. BRESLIN, M.B., C.D. GENG & W.V. VEDECKIS. 2001. Multiple promoters exist in the human GR gene, one of which is activated by glucocorticoids. Mol. Endocrinol. 15: 1381-1395.
2. TURNER, J.D. & C.P. MULLER. 2005. Structure of the glucocorticoid receptor (NR3C1) gene 5' untranslated region: identification, and tissue distribution of multiple new human exon 1. J. Mol. Endocrinol. 35: 283-292.
3. TOWBIN, H., T. STAEHELIN & J. GORDON. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.
4. NEWTON, C.R. et al. 1989. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 17: 2503-2516.
5. GENG, C.D. et al. 2005. Human glucocorticoid receptor alpha transcript splice variants with exon 2 deletions: evidence for tissue- and cell type-specific functions. Biochemistry 44: 7395-7405.
6. GENG, C.D. & W.V. VEDECKIS. 2005. c-Myb and members of the c-Ets family of transcription factors act as molecular switches to mediate opposite steroid regulation of the human glucocorticoid receptor 1A promoter. J. Biol. Chem. 280: 43264-43271.
7. NUNEZ, B.S. et al. 2005. Interaction between the interferon signaling pathway and the human glucocorticoid receptor gene 1A promoter. Endocrinology 146: 1449-1457.
8. MITTELSTADT, P.R. & J.D. ASHWELL. 2003. Disruption of glucocorticoid receptor exon 2 yields a ligand-responsive C-terminal fragment that regulates gene expression. Mol. Endocrinol. 17: 1534-1542.
9. HOLLENBERG, S.M. & R.M. EVANS. 1988. Multiple and cooperative transactivation domains of the human glucocorticoid receptor. Cell 55: 899-906.
10. MCKENNA, N.J. & B.W. O'MALLEY. 2002. Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108: 465-474.
11. MCKENNA, N.J. et al. 1999. Nuclear receptor coactivators: multiple enzymes, multiple complexes, multiple functions. J. Steroid Biochem. Mol. Biol. 69: 3-12.
12. MCKENNA, N.J., R.B. LANZ & B.W. O'MALLEY. 1999. Nuclear receptor coregulators: cellular and molecular biology. Endocr. Rev. 20: 321-344.