Development of an advanced electrochemical DNA biosensor for bacterial pathogen detection

Journal of Molecular Diagnostics

Volumn 9, Issue 2, 2007, Pages 158-168

  Liao, Joseph C ^a   Mastali, Mitra ^a,b   Li, Yang ^a   Gau, Vincent ^c   Suchard, Marc A ^a   Babbitt, Jane ^a,b   Gornbein, Jeffrey ^a   Landaw, Elliot M ^a   McCabe, Edward R B ^a   Churchill, Bernard M ^a   Haake, David A ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b VA GREATER LOS ANGELES HEALTHCARE SYSTEM   (United States)

^c GeneFluidics Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABCIXIMAB; BACTERIAL DNA; BIOTIN; FLUORESCEIN; HORSERADISH PEROXIDASE; LYSOZYME; NUCLEOTIDE; RNA 16S; TRITON X 100;

ARTICLE; BACTERIOLYSIS; BACTERIUM DETECTION; BIOASSAY; BIOSENSOR; CONTROLLED STUDY; ELECTROCHEMICAL ANALYSIS; ENZYME ACTIVITY; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HYBRIDIZATION; INTERMETHOD COMPARISON; NONHUMAN; OLIGONUCLEOTIDE PROBE; OXIDATION REDUCTION REACTION; SENSITIVITY ANALYSIS; URINALYSIS; BACTERIUM; DNA PROBE; ELECTROCHEMISTRY; GENETIC PROCEDURES; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE;

ARMORACIA RUSTICANA; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

BACTERIA; BACTERIOLYSIS; BIOSENSING TECHNIQUES; DNA PROBES; DNA, BACTERIAL; ELECTROCHEMISTRY; FLUORESCEIN; NUCLEIC ACID HYBRIDIZATION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

EID: 34247855137     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2007.060052     Document Type: Article

References (24)

1. Drummond TG, Hill MG, Barton JK: Electrochemical DNA sensors. Nat Biotechnol 2003, 21:1192-1199
2. Gau JJ, Lan EH, Dunn B, Ho CM, Woo JC: A MEMS based amperometric detector for E. coli bacteria using self-assembled monolayers. Biosens Bioelectron 2001, 16:745-755
3. Gooding JJ: Electrochemical DNA hybridization biosensor. Electroanalysis 2002, 14:1149-1156
4. Palecek E, Jelen F: Electrochemistry of nucleic acids and development of DNA sensors. Crit Rev Anal Chem 2002, 3:261-270
5. Wang J: Electrochemical nucleic acid biosensors. Anal Chim Acta 2002, 469:63-71
6. Griebling TL: Urologic diseases in America project: trends in resource use for urinary tract infections in men. J Urol 2005, 173:1288-1294
7. Griebling TL: Urologic diseases in America project: trends in resource use for urinary tract infections in women. J Urol 2005, 173:1281-1287
8. Gau V, Ma SC, Wang H, Tsukuda J, Kibler J, Haake DA: Electrochemical molecular analysis without nucleic acid amplification. Methods 2005, 37:73-83
9. Campbell CN, Gal D, Cristler N, Banditrat C, Heller A: Enzyme-amplified amperometric sandwich test for RNA and DNA. Anal Chem 2002, 74:158-162
10. Dequaire M, Heller A: Screen printing of nucleic acid detecting carbon electrodes. Anal Chem 2002, 74:4370-4377
11. Umek RM, Lin SW, Vielmetter J, Terbrueggen RH, Irvine B, Yu CJ, Kayyem JF, Yowanto H, Blackburn GF, Farkas DH, Chen YP: Electronic detection of nucleic acids: a versatile platform for molecular diagnostics. J Mol Diagn 2001, 3:74-84
12. Williams E, Pividori MI, Merkoci A, Forster RJ, Alegret S: Rapid electrochemical genosensor assay using a streptavidin carbon-polymer biocomposite electrode. Biosens Bioelectron 2003, 19:165-175
13. Fanjul-Bolado P, Gonzalez-Garcia MB, Costa-Garcia A: Amperometric detection in TMB/HRP-based assays. Anal Bioanal Chem 2005, 382:297-302
14. Mecheri B, Piras L, Ciotti L, Caminati G: Electrode coating with ultrathin films containing electroactive molecules for biosensor applications. IEEE Sens J 2004, 4:171-179
15. Sun CP, Liao JC, Zhang YH, Gau V, Mastali M, Babbitt JT, Grundfest WS, Churchill BM, McCabe ER, Haake DA: Rapid, species-specific detection of uropathogen 16S rDNA and rRNA at ambient temperature by dot-blot hybridization and an electrochemical sensor array. Mol Genet Metab 2005, 84:90-99
16. Liao JC, Mastali M, Gau V, Suchard MA, Moller AK, Bruckner DA, Babbitt JT, Li Y, Gornbein J, Landaw EM, McCabe ER, Churchill BM, Haake DA: Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens. J Clin Microbiol 2006, 44:561-570
17. Brosius J, Palmer ML, Kennedy PJ, Noller HF: Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 1978, 75:4801-4805
18. Summanen PH, Durmaz B, Vaisanen ML, Liu C, Molitoris D, Eerola E, Helander IM, Finegold SM: Porphyromonas somerae sp. nov., a pathogen isolated from humans and distinct from porphyromonas levii. J Clin Microbiol 2005, 43:4455-4459
19. Bard AJ, Faulkner LR: Potential Sweep Methods. Hoboken, NJ, John Wiley & Sons, 2001, pp 226-260
20. Chassy BM, Giuffrida A: Method for the lysis of Gram-positive, asporogenous bacteria with lysozyme. Appl Environ Microbiol 1980, 39:153-158
21. Fuchs BM, Glockner FO, Wulf J, Amann R: Unlabeled helper oligonucleotides increase the in situ accessibility to 16S rRNA of fluorescently labeled oligonucleotide probes. Appl Environ Microbiol 2000, 66:3603-3607
22. Metfies K, Huljic S, Lange M, Medlin LK: Electrochemical detection of the toxic dinoflagellate Alexandrium ostenfeldii with a DNA-biosensor. Biosens Bioelectron 2005, 20:1349-1357
23. O'Meara D, Nilsson P, Nygren PA, Uhlen M, Lundeberg J: Capture of single-stranded DNA assisted by oligonucleotide modules. Anal Biochem 1998, 255:195-203
24. Niemeyer CM, Bürger W, Peplies J: Covalent DNA-strepavidien conjugates as building blocks for novel biometallic nanostructures. Angew Chem Int Ed 1998, 37:2265-2268