Rapid, species-specific detection of uropathogen 16S rDNA and rRNA at ambient temperature by dot-blot hybridization and an electrochemical sensor array

Molecular Genetics and Metabolism

Volumn 84, Issue 1, 2005, Pages 90-99

  Sun, Chien Pin ^a   Liao, Joseph C ^b   Zhang, Yao Hua ^b   Gau, Vincent ^c   Mastali, Mitra ^b,d   Babbitt, Jane T ^b,d   Grundfest, Warren S ^a   Churchill, Bernard M ^b   McCabe, Edward R B ^b   Haake, David A ^b,d  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c GeneFluidics Inc   (United States)

^d Section of Infectious Diseases   (United States)

Author keywords

16S; Bacteria; Diagnosis; DNA probes; Microbiology; Molecular; Molecular diagnosis; Molecular microbiology; Nucleic acid hybridization methods; Oligonucleotide probes; RNA, Ribosomal; Urinary tract infections

Indexed keywords

DNA 16S; HORSERADISH PEROXIDASE; RIBOSOME RNA;

ARTICLE; BACTERIUM DETECTION; DNA BINDING; DNA PROBE; DOT HYBRIDIZATION; ELECTRONIC SENSOR; ENVIRONMENTAL TEMPERATURE; ESCHERICHIA COLI; GRAM NEGATIVE AEROBIC RODS AND COCCI; GRAM NEGATIVE ANAEROBIC COCCI; KLEBSIELLA OXYTOCA; NONHUMAN; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEUS MIRABILIS; PSEUDOMONAS AERUGINOSA; ROOM TEMPERATURE; SPECIES DIFFERENCE; TEMPERATURE DEPENDENCE; URINARY TRACT INFECTION;

BACTERIA; BASE SEQUENCE; COMMUNICABLE DISEASES; ELECTROCHEMISTRY; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SCINTILLATION COUNTING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

ARMORACIA RUSTICANA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA OXYTOCA; MIRABILIS; NEGIBACTERIA; POSIBACTERIA; PROTEUS MIRABILIS; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 19944427815     PISSN: 10967192     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymgme.2004.11.006     Document Type: Article

References (23)

1. C. Kunin Urinary Tract Infections: Detection, Prevention, and Management 1997 Williams & Wilkins Baltimore 78 101
2. J. Eisenstadt, and J. Washington H. Mobley J. Warren Urinary Tract Infections: Molecular Pathogenesis and Clinical Management 1996 ASM Press Washington, DC 29 66
3. W.E. Stamm Am. J. Med. 113 Suppl. 1A 2002 1S 4S
4. D. Relman, T. Schmidt, R. MacDermott, and S. Falkow N. Engl. J. Med. 327 1992 293 301
5. D. Relman Science 284 1999 1308 1310
6. I. Kroes, P.W. Lepp, and D.A. Relman Proc. Natl. Acad. Sci. USA 96 1999 14547 14552
7. M.A. Tanner, D. Shoskes, A. Shahed, and N.R. Pace J. Clin. Microbiol. 37 1999 1863 1870
8. T.G. Drummond, M.G. Hill, and J.K. Barton Nat. Biotechnol. 21 2003 1192 1199
9. J. Wang Anal. Chim. Acta 347 1997 1 8
10. J. Albers, T. Grunwald, E. Nebling, G. Piechotta, and R. Hintsche Anal. Bioanal. Chem. 377 2003 521 527
11. F. Martineau, F. Picard, C. Menard, P. Roy, M. Ouellette, and M. Bergeron J. Clin. Microbiol. 38 2000 3280 3284
12. K. Rose, J.O. Mason, and R. Lathe Biotechniques 33 2002 54 56 58
13. J. Kellokoski, S. Syrjanen, M. Yliskoski, and K. Syrjanen Oral Microbiol. Immunol. 7 1992 19 23
14. N.J. Fuller, D. Marie, F. Partensky, D. Vaulot, A.F. Post, and D.J. Scanlan Appl. Environ. Microbiol. 69 2003 2430 2443
15. A. Manero, and A.R. Blanch J. Microbiol. Methods 50 2002 115 121
16. K. Greisen, M. Loeffelholz, A. Purohit, and D. Leong J. Clin. Microbiol. 32 1994 335 351
17. V.J. Gau, E. Lan, B. Dunn, C.M. Ho, and J.C.S. Woo Biosen. Bioelectron. 16 2001 745 755
18. K. McCabe, G. Khan, Y. Zhang, E. Mason, and E. McCabe Pediatrics 95 1995 165 169
19. K.M. McCabe, Y.H. Zhang, B.L. Huang, E.A. Wagar, and E.R. McCabe Mol. Genet. Metab. 66 1999 205 211
20. J. Wang Nucleic Acids Res. 28 2000 3011 3016
21. B.M. Fuchs, G. Wallner, W. Beisker, I. Schwippl, W. Ludwig, and R. Amann Appl. Environ. Microbiol. 64 1998 4973 4982
22. S.J. Lee, and S.Y. Lee Appl. Microbiol. Biotechnol. 64 2004 289 299
23. Y. Van de Peer, S. Nicolai, P. De Rijk, and R. De Wachter Nucleic Acids Res. 24 1996 86 91