Expression and purification of HtpX-like small heat shock integral membrane protease of an unknown organism related to Methylobacillus flagellatus

Journal of Biochemical and Biophysical Methods

Volumn 70, Issue 4, 2007, Pages 539-546

  Siddiqui, Asim A ^a   Jalah, Rashmi ^a   Sharma, Yagya D ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Anaerobic bacteria; Heat shock protein; HPLC; HtpX like protein; Metalloprotease; Methylobacillus

Indexed keywords

CASEIN; HEAT SHOCK PROTEIN; HISTIDINE; METALLOPROTEINASE; PROTEIN HTPX; PROTEINASE; UNCLASSIFIED DRUG; ZINC ION;

ANAEROBIC BACTERIUM; ARTICLE; CATION EXCHANGE; CELL INCLUSION; ENZYME ACTIVITY; ESCHERICHIA COLI; GEL FILTRATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; METHYLOBACILLUS FLAGELLATUS; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN PURIFICATION; SOLUBILITY; ZYMOGRAPHY;

BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA PRIMERS; HEAT-SHOCK PROTEINS; INCLUSION BODIES; KINETICS; METHYLOBACILLUS; PROTEIN FOLDING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; METHYLOBACILLUS; METHYLOBACILLUS FLAGELLATUS;

EID: 34247562745     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2006.12.002     Document Type: Article

References (26)

1. Gottesman S. Proteases and their targets in Escherichia coli. Annu Rev Genet 30 (1996) 465-506
2. Kornitzer D., Teff D., Altuvia S., and Oppenheim A.B. Isolation, characterization and sequence of an Escherichia coli heat shock gene, HtpX. J Bacteriol 173 (1991) 2944-2953
3. Sakoh M., Ito K., and Akiyama Y. Proteolytic activity of HtpX, a membrane-bound and stress-controlled protease from E. coli. J Biol Chem 280 (2005) 33305-33310
4. Rawlings N.D., and Barrett A.J. Evolutionary families of metallopeptidases. Methods Enzymol 248 (1995) 183-228
5. Akiyama Y., Ogura T., and Ito K. FtsH protease. In: Barret A.J., Rawlings N.D., and Woessner J.F. (Eds). Handbook of Proteolytic Enzymes. second Ed. (2004), Academis Press, London
6. Fujimura-kamada K., Nouvet F.J., and Michaelis S. A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor. J Cell Biol 136 (1997) 271-285
7. Fakruddin J.M., Biswas S., and Sharma Y.D. Identification of a Plasmodium vivax heat-shock protein which contains a metalloprotease sequence motif. Mol Biochem Parasitol 90 (1997) 387-390
8. Fakruddin J.M., Biswas S., and Sharma Y.D. Metalloprotease activity in a small heat shock protein of the human malaria parasite Plasmodium vivax. Infect Immun 68 (2000) 1202-1206
9. Halbhuber Z., Petrmichlova Z., Alexciev K., Thulin E., and Stys D. Overexpression and purification of recombinant membrane PsbH protein in Escherichia coli. Protein Expr Purif 32 (2003) 18-27
10. Sambrook T., and Maniatis. Molecular cloning: a laboratory manual (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
11. Burgess R.R. Purification of overproduced Escherichia coli RNA polymerase σ factors by solubilizing inclusion bodies and refolding from Sarkosyl. Methods Enzymol 273 (1996) 145-149
12. Kurucz I., Titus J.A., Jost C.R., and Segel D.M. Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies. Mol Immunol 32 (1995) 1443-1452
13. Heussen C., and Dowdle E.B. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem 102 (1980) 196-202
14. Auer M., Kim M.J., Lemieux M.J., Villa A., Song J., Dan Li X., and Wang D.N. High-yield expression and functional analysis of Escherichia coli glycerol-3-phosphate transporter. Biochemistry 40 (2001) 6628-6635
15. Wunderli-Ye H., and Solioz M. Purification and functional analysis of the copper ATPase CopA of Enterococcus hirae. Biochem Biophy Res Commun 280 (2001) 713-719
16. Vercaigne-Marko D., Kosciarz E., Nedjar-Arroume N., and Guillochon D. Improvement of Staphylococcus aureus-V8-protease hydrolysis of bovine haemoglobin by its adsorption on to a solid phase in the presence of SDS: peptide mapping and obtention of two haemopoietic peptides. Biotechnol Appl Biochem 31 (2000) 127-134
17. Anwar A., and Saleemuddin M. Alkaline protease from Spilosoma obliqua: potential applications in bio-formulations. Biotechnol Appl Biochem 31 (2000) 85-89
18. Najafi M.F., Deobagkar D., and Deobagkar D. Potential application of protease isolated from Pseudomonas aeruginosa. Electron J Biotechnol 8 (2005) 197-203
19. Larsen B.S., and Biedermann V. Isolation and characterization of the outer membrane proteins of Serratia marcescens W225. Anal Biochem 214 (1993) 212-221
20. Ravaoarinoro M., Ciurli C., Toma E., and Morisset R. Rapid method for isolating detergent-insoluble outer membrane proteins from Pseudomonas aeruginosa. Electrophoresis 15 (1994) 594-596
21. Farizo K.M., Fiddner S., Cheung A.M., and Burns D.L. Membrane localization of the S1 subunit of pertussis toxin in Bordetella pertussis and implications for pertussis toxin secretion. Infect Immun 70 (2002) 1193-1201
22. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25 (1997) 3389-3402
23. Kothekar V., Shankar S., Lomash S., and Sharma Y.D. Computer modeling of small heat-shock metalloprotease of the human malaria parasite Plasmodium vivax. J Biomol Struct Dyn 19 (2001) 201-213
24. Bonander N., Hedfalk K., Larsson C., Mostad P., Chang C., Gustafsson L., et al. Design of improved membrane protein production experiments: quantitation of the host response. Protein Sci 14 (2005) 1729-1740
25. Rudolph R., and Lilie H. In vitro folding of inclusion body proteins. FASEB J 10 (1996) 49-56
26. Rudolph R., Böhm G., Lilie H., and Jaenicke R. In: Creighton T.E. (Ed). In Protein function: A Practical Approach (1997), IRL Press 57-99