Screening and purification for novel cytochrome b5 from uncultured environmental micro-organisms

Letters in Applied Microbiology

Volumn 44, Issue 5, 2007, Pages 475-480

  Roh, C ^a,b   Villatte, F ^b   Kim, B G ^a   Schmid, R D ^b  

^a Seoul National University   (South Korea)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Cytochrome b5; Sequence based approach; Uncultivation

Indexed keywords

CLONING; DNA; ESCHERICHIA COLI; GENE ENCODING; POLYMERASE CHAIN REACTION; PURIFICATION;

CYTOCHROME B; CYTOCHROME B5; DEGENERATE PRIMERS; HEME-BINDING DOMAINS; METAGENOMIC DNA; MICRO-ORGANISMS; NOVEL GENES; PCR METHOD; SEQUENCE-BASED APPROACH; UNCULTIVATION;

AFFINITY CHROMATOGRAPHY;

CYTOCHROME B5; GENOMIC DNA; HEME; NITRILOTRIACETIC ACID; NITROGEN; RECOMBINANT ENZYME;

DNA; ENZYMES; ESCHERICHIA COLI; GENE ENCODING; GENETIC ENGINEERING;

BACTERIUM; CYTOCHROME; DNA; GENE; GENETIC ISOLATION; MICROORGANISM;

AFFINITY CHROMATOGRAPHY; ARTICLE; CYTOCHROME B5 GENE; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENE SEQUENCE; GENE TARGETING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; RHODOPSEUDOMONAS PALUSTRIS;

ESCHERICHIA COLI; RHODOPSEUDOMONAS PALUSTRIS;

EID: 34247402223     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02118.x     Document Type: Article

References (22)

1. Bell, P.J., Sunna, A., Gibbs, M.D., Curach, N.C., Nevalainen, H. Bergquist, P.L. (2002) Prospecting for novel lipase genes using PCR. Microbiology 148, 2283 2291.
2. Chen, Z. Banerjee, R. (1998) Purification of soluble cytochrome b5 as a component of the reductive activation of porcine methionine synthase. J Biol Chem 273, 26248 26255.
3. Courtois, S., Cappellano, C.M., Ball, M., Francou, F.X., Normand, P., Helynck, G., Martinez, A., Kolvek, S.J., et al. (2003) Recombinant environmental libraries provide access to microbial diversity for drug discovery form natural products. Appl Environ Microbiol 69, 45 55.
4. Elend, C., Schmeisser, C., Leggewie, C., Babiak, P., Carballeira, J.D., Steele, H.L., Reymond, J.-L., Jaeger, K.-E., et al. (2006) Isolation and biochemical characterization of two novel metagenome-derived esterases. Appl Environ Microbiol 72, 3637 3645.
5. Eschenfeldt, W.H., Stols, L., Rosenbaum, H., Khambatta, Z.S., Quaite-Randall, E., Wu, S., Kilgore, D.C., Trent, J.D., et al. (2001) DNA from uncultured organisms as a source of 2,5-diketo-D-gluconic acid reductases. Appl Environ Microbiol 67, 4206 4214.
6. Halvorson, M., Greenway, D., Eberhart, D., Fitzgerald, K. Parkinson, A. (1990) Reconstitution of testosterone oxidation by purified rat cytochrome P450p (IIIA 1). Arch Biochem Biophys 277, 166 180.
7. Jung, Y.W., Kwak, J.Y. Lee, Y.H. (2001) High-level production of heme-containing holoproteins in Escherichia coli. Appl Microbiol Biotechnol 55, 187 191.
8. LeCleir, G.R., Buchan, A. Hollibaugh, J.T. (2004) Chitinase gene sequences retrieved form diverse aquatic habitats reveal environment-specific distributions. Appl Environ Microbiol 70, 6977 6983.
9. Lee, S.W., Won, K., Lim, H.K., Kim, J.C., Choi, G.J. Cho, K.Y. (2004) Screening for novel lipolytic enzymes from uncultured soil microorganisms. Appl Microbiol Biotechnol 65, 720 726.
10. Liebeton, K. Juergen, E. (2004) Identification and expression in E. coli of novel nitrile hydratases from the metagenome. Eng Life Sci 4, 557 562.
11. Loughran, P.A., Roman, L.J., Miller, T. Masters, B.S. (2001) The kinetic and spectral characterization of the E. coli expressed mammalian CYP4A7: cytochrome b5 effects vary with substrate. Arch Biochem Biophys 385, 311 321.
12. Marchesi, J.R. Weightman, A.J. (2003) Comparing the dehalogenase gene pool in cultivated alpha-halocarboxylic acid-degrading bacteria with environmental metagene pool. Appl Environ Microbiol 69, 4375 4382.
13. Mitra, A., Chakrabarti, K.S., Hameed, M.S.S., Srinivas, K.V., Kumar, G.S. Sarma, S.P. (2005) High level expression of peptides and proteins using cytochrome b5 as a fusion host. Protein ExprPurif 41, 84 97.
14. Mulrooney, S.B. Waskell, L. (2000) High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b5. Protein Expr Purif 19, 173 178.
15. Murataliev, M.B., Feyereisen, R. Walker, F.A. (2004) Electron transfer by diflavin reductases. Biochim Biophys Acta 1698, 1 26.
16. Ortiz de Montellano, P.R. (2005) Cytochrome P450 Strucuture, Mechanism, and Biochemistry. New York : Kluwer Academic/Plenum Publisher.
17. Porter, T.D. (2002) The roles of cytochrome b5 in cytochrome P450 reactions. J Biochem Mol Toxico 16, 311 316.
18. Roh, C., Villatte, F., Kim, B.G. Schmid, R.D. (2005) In-gel patch electrophoresis: a new method for environmental DNA purification. Electrophoresis 26, 3055 3061.
19. Rondon, M.R., Goodman, R.M. Handelsman, J. (1999) The earth's bounty: assessing and accessing soil microbial diversity. Trends Biotechnol 17, 403 409.
20. Schenkman, J.B. Jansson, I. (2003) The many roles of cytochrome b5. Pharmacol Ther 97, 139 152.
21. Uchiyama, T., Abe, T., Ikemura, T. Watanabe, K. (2005) Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes. Nat Biotechnol 23, 88 93.
22. Whitman, W.B., Coleman, D.C. Wiebe, W.J. (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 95, 6578 6583.