Lack of p53 augments thymoquinone-induced apoptosis and caspase activation in human osteosarcoma cells

Cancer Biology and Therapy

Volumn 6, Issue 2, 2007, Pages 160-169

  Roepke, Martin ^a   Diestel, Antje ^a   Bajbouj, Khuloud ^a   Walluscheck, Diana ^a   Schonfeld, Peter ^a   Roessner, Albert ^a   Schneider Stock, Regine ^a   Gali Muhtasib, Hala ^b  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b AMERICAN UNIVERSITY OF BEIRUT   (Lebanon)

Author keywords

Apoptosis; Caspases; Osteosarcoma; Oxidative stress; Thymoquinone

Indexed keywords

ACONITATE HYDRATASE; CASPASE 3; CASPASE 9; CYCLIN DEPENDENT KINASE INHIBITOR 1; HISTONE H2AX; NIBRIN; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53; SUPEROXIDE; THYMOQUINONE;

ADULT; ANIMAL CELL; ANIMAL TISSUE; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL CYCLE ARREST; CELL POPULATION; CELL SURVIVAL; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; DNA DETERMINATION; DRUG MECHANISM; ENZYME ACTIVATION; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MITOCHONDRION; NONHUMAN; OSTEOBLAST; OSTEOSARCOMA CELL; OXIDATION; OXIDATIVE STRESS; PROTEIN EXPRESSION; RAT; UPREGULATION;

EID: 34247362432     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.6.2.3575     Document Type: Article

References (25)

1. Andreyev AYU, Kushnareva YUE, Starkov AA. Mitochondrial metabolism of reactive oxygen species. Biochemistry (Moscow) 2005; 70:246-64.
2. Worthen DR, Ghosheh OA, Crooks PA. The in vitro anti-tumor activity of some crude and purified components of blackseed, Nigella sativa L. Anti-cancer Res 1998; 18:1527-32.
3. Gali-Muhtasib H, Diab-Assaf M, Boltze C, Al-Hmaira J, Hartig R, Roessner A, Schneider-Stock R. Thymoquinone extracted from black seed triggers apoptotic cell death in human colorectal cancer cells via a p53-dependent mechanism. Int J Oncol 2004a; 25:857-66.
4. Gali-Muhtasib HU, Abou Kheir WG, Kheir LA, Darwiche N, Crooks PA. Molecular pathway for thymoquinone-induced cell-cycle arrest and apoptosis in neoplastic keratinocytes. Anti-cancer Drugs 2004b; 15:389-99.
5. Shoieb AM, Elgayyar M, Dudrick PS, Bell JL, Tithof PK. In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. Int J Oncol 2003; 22:107-13.
6. Badary OA, Nagi MN, al-Shabanah OA, al-Sawaf HA, al-Sohaibani MO, al-Bekairi AM. Thymoquinone ameliorates the nephrotoxicity induced by cisplatin in rodents and potentiates its antitumor activity. Can J Physiol Pharmacol 1997; 75:1356-61.
7. El-Mahdy MA, Zhu Q, Wang QE, Wani G, Wani AA. Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in p53-null myeloblastic leukemia HL-60 cells. Int J Cancer 2005; 117:409-17.
8. Gali-Muhtasib H, Roessner A, Schneider-Stock R. Thymoquinone: A promising anti-cancer drug from natural sources. Int J Biochem Cell Biol 2006; 38:1249-53.
9. Masuda H, Miller C, Koeffler HP, Battifora H, Cline MJ. Rearrangement of the p53 gene in human osteogenic sarcomas. Proc Natl Acad Sci USA 1987; 84:7716-9.
10. Iida K, Nobori T, Matsumine A, Isaka A, Seto M, Shiraishi T, Uchida A. Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines. Oncol Rep 2003; 10:1961-5.
11. Steinbrecht I, Kunz W. Use of "cycling" technic for random quantitative determination of the degree of reduction of NAD and NADP system in rat liver mitochondria with continuous recording of the measurements. Acta Biol Med Germ 1970; 25:731-47.
12. Gardner PR. Aconitase: Sensitive target and measure of superoxide. Methods Enzymol 2002; 349:9-23.
13. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998; 273:5858-68.
14. Nunez G, Benedict MA, Hu Y, Inohara N. Caspases: The proteases of the apoptotic pathway. Oncogene 1998; 17:3237-45.
15. Riedl SJ, Shi Y. Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol 2004; 5:897-907.
16. Gamen S, Anel A, Lasierra P, Alava MA, Martinez-Lorenzo MJ, Pineiro A, Naval J. Doxorubicin-induced apoptosis in human T-cell leukemia is mediated by caspase-3 activation in a Fas-independent way. FEBS Lett 1997; 417:360-4.
17. Sharpe JC, Arnoult D, Youle RJ. Control of mitochondrial permeability by Bcl-2 family members. Biochim Biophys Acta 2004; 1644:107-13.
18. Zhang Y, Fujita N, Tsuruo T. p21WAF1/Cip1 acts in synergy with bcl-2 to confer multidrug resistance in a camptothecin-selected human lung-cancer cell line. Int J Cancer 1999; 83:790-97.
19. Chang GC, Hsu SL, Tsai JR, Wu WJ, Chen CY, Sheu GT. Extracellular signal-regulated kinase activation and Bcl-2 downregulation mediate apoptosis after gemcitabine treatment partly via a p53-independent pathway. Eur J Pharmacol 2004; 502:169-83.
20. Badary OA, Taha RA, Gamal el-Din AM, Abdel-Wahab MH. Thymoquinone is a potent superoxide anion scavenger. Drug Chem Toxicol 2003; 26:87-98.
21. Al-Majed AA, Al-Omar FA, Nagi MN. Neuroprotective effects of thymoquinone against transient forebrain ischemia in the rat hippocampus. Eur J Pharmacol 2006; 543:40-7.
22. Kang J, Ferguson D, Song H, Bassing C, Eckersdorff M, Alt FW, Xu Y. Functional interaction of H2AX, NBS1, and p53 in ATM-dependent DNA damage responses and tumor suppression. Mol Cell Biol 2005; 25:661-70.
23. Porcedda P, Turinetto V, Lantelme E, Fontanello E, Chrzanowska K, Ragona R, DeMarchi M, Delia D, Giachino C. Impaired elemination of DNA double-strandbreak-containing lymphocytes in ataxia teleangiectasia and Nijmegen breakage sindrome. DNA Repair 2006; 5:904-13.
24. Xiao GH, Jeffers M, Bellacosa A, Mitsuuchi Y, Vande Woude GF, Testa JR. Antiapoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase pathways. PNAS 2001; 98:247-52.
25. Vafa O, Wade M, Kern S, Beeche M, Pandita TK, Hampton GM, Wahl GM. c-Myc can induce DNA-damage, increase reactive oxygen species, and mitigate p53 function: A mechanism for oncogene-induced genetic instability. Mol Cell 2002; 9:1031-44.