Thioredoxin-linked metabolism in Entamoeba histolytica

Free Radical Biology and Medicine

Volumn 42, Issue 10, 2007, Pages 1496-1505

  Arias, Diego G ^a   Gutierrez, César E ^a   Iglesias, Alberto A ^a   Guerrero, Sergio A ^a  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

Entamoeba histolytica; Free radicals; Redox metabolism; Structural and functional characterization; Thioredoxin system

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); GENOMIC DNA; HYDROGEN PEROXIDE; PEROXIREDOXIN; REACTIVE OXYGEN METABOLITE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TERT BUTYL HYDROPEROXIDE; THIOREDOXIN; THIOREDOXIN REDUCTASE;

ARTICLE; CONTROLLED STUDY; DETOXIFICATION; ENTAMOEBA HISTOLYTICA; ESCHERICHIA COLI; GENE; GENE EXPRESSION; HUMAN; MICHAELIS CONSTANT; MICROBIAL METABOLISM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN METABOLISM; PROTEIN PURIFICATION; PROTOZOAL GENETICS; TRX41 GENE; TRXR GENE;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; ENTAMOEBA HISTOLYTICA; ESCHERICHIA COLI; GENES, PROTOZOAN; GENOME, PROTOZOAN; HYDROGEN PEROXIDE; MOLECULAR SEQUENCE DATA; NADP; OXIDATION-REDUCTION; PROTOZOAN PROTEINS; SEQUENCE ALIGNMENT; THIOREDOXIN; THIOREDOXIN REDUCTASE (NADPH);

ENTAMOEBA HISTOLYTICA; ESCHERICHIA COLI; PROTOZOA;

EID: 34247127727     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2007.02.012     Document Type: Article

References (43)

1. Bhattacharya A., Satish S., Bagchi A., and Bhattacharya S. The genome of Entamoeba histolytica. Int. J. Parasitol. 30 (2000) 401-410
2. Ondarza R.N., Iturbe A., Hurtado G., Tamayo E., Ondarza M., and Hernandez E. Entamoeba histolytica: a eukaryote with trypanothione metabolism instead of glutathione metabolism. Biotechnol. Appl. Biochem. 30 Pt 1 (1999) 47-52
3. Loftus B., Anderson I., Davies R., Alsmark U.C., Samuelson J., Amedeo P., Roncaglia P., Berriman M., Hirt R.P., Mann B.J., Nozaki T., Suh B., Pop M., Duchene M., Ackers J., Tannich E., Leippe M., Hofer M., Bruchhaus I., Willhoeft U., Bhattacharya A., Chillingworth T., Churcher C., Hance Z., Harris B., Harris D., Jagels K., Moule S., Mungall K., Ormond D., Squares R., Whitehead S., Quail M.A., Rabbinowitsch E., Norbertczak H., Price C., Wang Z., Guillen N., Gilchrist C., Stroup S.E., Bhattacharya S., Lohia A., Foster P.G., Sicheritz-Ponten T., Weber C., Singh U., Mukherjee C., El-Sayed N.M., Petri Jr. W.A., Clark C.G., Embley T.M., Barrell B., Fraser C.M., and Hall N. The genome of the protist parasite Entamoeba histolytica. Nature 433 (2005) 865-868
4. Bruchhaus I., Richter S., and Tannich E. Characterization of two E. histolytica proteins that inactivate reactive oxygen species. Arch. Med. Res. 28 (1997) 91-92 (Spec Issue)
5. Choi M.H., Sajed D., Poole L., Hirata K., Herdman S., Torian B.E., and Reed S.L. An unusual surface peroxiredoxin protects invasive Entamoeba histolytica from oxidant attack. Mol. Biochem. Parasitol. 143 (2005) 80-89
6. Ariyanayagam M.R., and Fairlamb A.H. Entamoeba histolytica lacks trypanothione metabolism. Mol. Biochem. Parasitol. 103 (1999) 61-69
7. Fahey R.C., Newton G.L., Arrick B., Overdank-Bogart T., and Aley S.B. Entamoeba histolytica: a eukaryote without glutathione metabolism. Science 224 (1984) 70-72
8. Tamayo E.M., Iturbe A., Hernandez E., Hurtado G., de Lourdes Gutierrez X.M., Rosales J.L., Woolery M., and Ondarza R.N. Trypanothione reductase from the human parasite Entamoeba histolytica: a new drug target. Biotechnol. Appl. Biochem. 41 (2005) 105-115
9. Ondarza R.N., Hernandez E., Iturbe A., Hurtado G., and Tamayo E.M. Detection by HPLC of a trypanothione synthetase activity in vitro from Entamoeba histolytica. Biotechnol. Appl. Biochem. 30 Pt 1 (1999) 41-45
10. Ondarza R.N., Hurtado G., Iturbe A., Hernandez E., Tamayo E., and Woolery M. Identification of trypanothione from the human pathogen Entamoeba histolytica, by mass spectrometry and chemical analysis. Biotechnol. Appl. Biochem. 42 (2005) 175-181
11. Mehlotra R.K. Parasitic protozoa: thiol-based redox metabolism. Trends Parasitol. 20 (2004) 58-59
12. Tekwani B.L., and Mehlotra R.K. Molecular basis of defence against oxidative stress in Entamoeba histolytica and Giardia lamblia. Microbes Infect. 1 (1999) 385-394
13. Akbar M.A., Chatterjee N.S., Sen P., Debnath A., Pal A., Bera T., and Das P. Genes induced by a high-oxygen environment in Entamoeba histolytica. Mol. Biochem. Parasitol. 133 (2004) 187-196
14. Tannich E., Bruchhaus I., Walter R.D., and Horstmann R.D. Pathogenic and nonpathogenic Entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase. Mol. Biochem. Parasitol. 49 (1991) 61-71
15. Bruchhaus I., Richter S., and Tannich E. Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica. Biochem. J. 326 Pt 3 (1997) 785-789
16. Bruchhaus I., and Tannich E. Analysis of the genomic sequence encoding the 29-kDa cysteine-rich protein of Entamoeba histolytica. Trop. Med. Parasitol. 44 (1993) 116-118
17. Lo H., and Reeves R.E. Purification and properties of NADPH:flavin oxidoreductase from Entamoeba histolytica. Mol. Biochem. Parasitol. 2 (1980) 23-30
18. Bruchhaus I., Richter S., and Tannich E. Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica. Biochem. J. 330 Pt 3 (1998) 1217-1221
19. Reckenfelderbaumer N., Ludemann H., Schmidt H., Steverding D., and Krauth-Siegel R.L. Identification and functional characterization of thioredoxin from Trypanosoma brucei brucei. J. Biol. Chem. 275 (2000) 7547-7552
20. Piattoni C.V., Blancato V.S., Miglietta H., Iglesias A.A., and Guerrero S.A. On the occurrence of thioredoxin in Trypanosoma cruzi. Acta Trop. 97 (2006) 151-160
21. Krauth-Siegel R.L., Bauer H., and Schirmer R.H. Dithiol proteins as guardians of the intracellular redox milieu in parasites: old and new drug targets in trypanosomes and malaria-causing plasmodia. Angew. Chem. Int. Ed. Engl. 44 (2005) 690-715
22. Maniatis T.F., Fritsch E.F., and Sambrook J. Molecular Cloning: A Laboratory Manual (1982), Cold Spring Harbor, NY: Laboratory Press;, Cold Spring Harbor, NY
23. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
24. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
25. Vaitukaitis J., Robbins J.B., Nieschlag E., and Ross G.T. A method for producing specific antisera with small doses of immunogen. J. Clin. Endocrinol. Metab. 33 (1971) 988-991
26. Holmgren A. Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide. J. Biol. Chem. 254 (1979) 9627-9632
27. Holmgren A. Reduction of disulfides by thioredoxin: exceptional reactivity of insulin and suggested functions of thioredoxin in mechanism of hormone action. J. Biol. Chem. 254 (1979) 9113-9119
28. Bruchhaus I., Brattig N.W., and Tannich E. Recombinant expression, purification and biochemical characterization of a superoxide dismutase from Entamoeba histolytica. Arch. Med. Res. 23 (1992) 27-29
29. Coombs G.H., Westrop G.D., Suchan P., Puzova G., Hirt R.P., Embley T.M., Mottram J.C., and Muller S. The amitochondriate eukaryote Trichomonas vaginalis contains a divergent thioredoxin-linked peroxiredoxin antioxidant system. J. Biol. Chem. 279 (2004) 5249-5256
30. Williams C.H., Arscott L.D., Muller S., Lennon B.W., Ludwig M.L., Wang P.F., Veine D.M., Becker K., and Schirmer R.H. Thioredoxin reductase: two modes of catalysis have evolved. Eur. J. Biochem. 267 (2000) 6110-6117
31. Holmgren A., Soderberg B.O., Eklund H., and Branden C.I. Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution. Proc. Natl. Acad. Sci. USA 72 (1975) 2305-2309
32. MacFarlane R.C., and Singh U. Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: potential implications for amebic pathogenesis. Infect. Immun. 74 (2006) 340-351
33. Loftus B.J., and Hall N. Entamoeba: still more to be learned from the genome. Trends Parasitol. 21 (2005) 453
34. 1H NMR. Biochemistry 30 (1991) 4262-4268
35. Hirt R.P., Muller S., Embley T.M., and Coombs G.H. The diversity and evolution of thioredoxin reductase: new perspectives. Trends Parasitol. 18 (2002) 302-308
36. Eckburg P.B., Bik E.M., Bernstein C.N., Purdom E., Dethlefsen L., Sargent M., Gill S.R., Nelson K.E., and Relman D.A. Diversity of the human intestinal microbial flora. Science 308 (2005) 1635-1638
37. Orozco E., Guarneros G., Martinez-Palomo A., and Sanchez T. Entamoeba histolytica: phagocytosis as a virulence factor. J. Exp. Med. 158 (1983) 1511-1521
38. Rosenbaum R.M., and Wittner M. Ultrastructure of bacterized and axenic trophozoites of Entamoeba histolytica with particular reference to helical bodies. J. Cell Biol. 45 (1970) 367-382
39. Wittner M., and Rosenbaum R.M. Role of bacteria in modifying virulence of Entamoeba histolytica: studies of amebae from axenic cultures. Am. J. Trop. Med. Hyg. 19 (1970) 755-761
40. Holmgren A., Ohlsson I., and Grankvist M.L. Thiroedoxin from Escherichia coli: radioimmunological and enzymatic determinations in wild type cells and mutants defective in phage T7 DNA replication. J. Biol. Chem. 253 (1978) 430-436
41. Gleason F.K. Mutation of conserved residues in Escherichia coli thioredoxin: effects on stability and function. Protein Sci. 1 (1992) 609-616
42. Bracha R., and Mirelman D. Virulence of Entamoeba histolytica trophozoites: effects of bacteria, microaerobic conditions, and metronidazole. J. Exp. Med. 160 (1984) 353-368
43. Schmidt H., and Krauth-Siegel R.L. Functional and physicochemical characterization of the thioredoxin system in Trypanosoma brucei. J. Biol. Chem. 278 (2003) 46329-46336