Evaluation of catechol-induced DNA damage in human lymphocytes: A comparison between freshly isolated lymphocytes and T-lymphocytes from extended-term cultures

Toxicology in Vitro

Volumn 21, Issue 4, 2007, Pages 716-722

  Andersson, Maria A ^a   Hellman, Björn E ^a  

^a Department of Pharmaceutical Biosciences   (Sweden)

Author keywords

Catechol; Comet assay; DNA strand breaks; Extended term cultures of human T lymphocytes; Freshly isolated human blood lymphocytes; Hydrogen peroxide; Oxidative DNA damage; Reactive oxygen species

Indexed keywords

CATECHOL; REACTIVE OXYGEN METABOLITE;

ARTICLE; CELL ISOLATION; CELL PROLIFERATION; CONTROLLED STUDY; DNA DAMAGE; GENOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOCYTE; T LYMPHOCYTE;

ADULT; CATECHOLS; CELL SEPARATION; CELLS, CULTURED; COMET ASSAY; DNA DAMAGE; FEMALE; HUMANS; HYDROGEN PEROXIDE; LYMPHOCYTES; MALE; MIDDLE AGED; OXIDATION-REDUCTION; PURINES; PYRIMIDINES; REACTIVE OXYGEN SPECIES; T-LYMPHOCYTES;

EID: 34147116807     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2006.12.012     Document Type: Article

References (28)

1. Anderson D., Yu T.W., Phillips B.J., and Schmezer P. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutation Research 307 (1994) 261-271
2. Andersson M.A., and Hellman B.E. Different roles of Fpg and Endo III on catechol-induced DNA damage in extended-term cultures of human lymphocytes and L5178Y mouse lymphoma cells. Toxicology In Vitro 19 (2005) 779-786
3. Andersson M., Agurell E., Vaghef H., Bolcsfoldi G., and Hellman B. Extended-term cultures of human lymphocytes and the comet assay: a useful combination when testing for genotoxicity in vitro?. Mutation Research 540 (2003) 43-55
4. Andersson, M.A., Petersson, K.V., Grawé, O.M., Karlsson, L.A.G., Abramsson-Zetterberg, Hellman, B.E., in press. Evaluation of the potential genotoxicity of chromium picolinate in mammalian cells in vivo and in vitro. Food & Chemical Toxicology, doi:10.1016/j.fct.2006.11.008.
5. Bartosova Z., Pirsel M., Reinhold W., Stetler-Stevenson M., Zajac-Kaye M., May A., Horak I.D., and Bohr V.A. Gene-specific repair in human CD4+ lymphocytes reflects transcription and proliferation. Mutation Research 363 (1996) 191-199
6. Bohr V.A., Evans M.K., and Fornace Jr. A.J. DNA repair and its pathogenetic implications. Laboratory Investigations 61 (1989) 143-161
7. Brandt R., and Keston A.S. Synthesis of diacetyldichlorofluorescin: a stable reagent for fluorometric analysis. Analytical Biochemistry 11 (1965) 6-9
8. Carson D.A., Carrera D.A., Wasson D.B., and Yamanaka H. Programmed cell death and adenine deoxynucleotide metabolism in human lymphocytes. Advances in Enzyme Regulation 27 (1988) 395-404
9. Cathcart R., Schwiers E., and Ames B.N. Detection of picomole levels of hydroperoxides using the fluorescent dichlorofluorescein assay. Analytical Biochemistry 134 (1983) 111-116
10. Collins A.R., Dusinska M.M., Horvathova E., Munro E., Savio M., and Stetina R. Inter- individual differences in repair of DNA base oxidation, measured in vitro with the comet assay. Mutagenesis 16 (2001) 297-301
11. Hellman B., Vaghef H., and Boström B. The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutation Research 336 (1995) 123-131
12. Hellman B., Vaghef H., Friis L., and Edling C. Alkaline single cell gel electrophoresis of DNA fragments in biomonitoring for genotoxicity: An introductory study on healthy human volunteers. International Archives of Occupational and Environmental Health 69 (1997) 185-192
13. Hirakawa K., Oikawa S., Hiraku Y., Hirosawa I., and Kawanishi S. Catechol and hydroquinone have different redox properties responsible for their differential DNA-damaging ability. Chemical Research in Toxicology 15 (2002) 76-82
14. Hiraku Y., and Kawanishi S. Oxidative DNA damage and apoptosis induced by benzene Metabolites. Cancer Research 56 (1996) 5172-5178
15. Holz O., Jörres R., Kästner A., Krause T., and Magnussen H. Reproducibility of basal and induced DNA single-strand breaks detected by the single-cell gel electrophoresis assay in human peripheral mononuclear leukocytes. International Archives of Occupational and Environmental Health 67 (1995) 305-310
16. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans: Re-evaluation of Some Organic Chemicals, Hydrazine and Hydrogen Peroxide, vol. 71, International Agency for Research on Cancer, Lyon, 1999, pp. 433-451.
17. Jakubowski W., and Bartosz G. 2,7-dichlorofluorescin oxidation and reactive oxygen species: what does it measure?. Cellular and Biological Interactions 24 (2000) 757-760
18. Janssen K., Eichhorn-Grombacher U., Schlink K., Nitzsche S., Oesch F., and Kaina B. Long-time expression of DNA repair enzymes MGMT and APE in human peripheral blood mononuclear cells. Archives of Toxicology 75 (2001) 306-312
19. Keston A.S., and Brandt R. The fluorometric analysis of ultra micro quantities of hydrogen peroxide. Analytical Biochemistry 11 (1965) 1-5
20. O'Donovan M.R.O., Freemantle M.R., Hull G., Bell D.A., Arlett C.F., and Cole J. Extended-term cultures of human lymphocytes: a practical alternative to primary human lymphocytes for use in genotoxicity testing. Mutagenesis 10 (1995) 189-201
21. Oikawa S., Hirosawa I., Hirakawa H., and Kawanishi S. Site specificity and mechanism of oxidative DNA damage induced by carcinogenic catechol. Carcinogenesis 22 (2001) 1239-1245
22. Östling O., and Johanson K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemistry and Biophysics Research Communication 123 (1984) 291-298
23. Pero R.W., Bryngelsson C., Mitelman F., Kornfält R., Thulin T., and Norden Å. Interindividual variation in the responses of cultured human lymphocytes to exposure from DNA damaging agents. Mutation Research 53 (1978) 327-341
24. Schweigert N., Acero J.L., von Gunten U., Canonica S., Zehnder A.J.B., and Eggen R.I.L. DNA degradation by the mixture of copper and catechol is caused by DNA-copper-hydroperoxo complexes, probably DNA-Cu(I)OOH. Environmental Molecular Mutagenesis 36 (2000) 5-12
25. Schweigert N., Zehnder A.J., and Eggen R.I. Chemical properties of catechols and their molecular modes of toxic action in cells, from micro organisms to mammals. Environmental Microbiology 3 (2001) 81-92
26. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantification of low levels of DNA damage in individual cells. Experimental Cell Research 175 (1988) 184-191
27. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann H., Kobayashi Y., Miyamae Y., Rojas E., Ryu J.-C., and Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis 35 (2000) 206-221
28. Vaghef H., Wisén A.-C., and Hellman B. Demonstration of benzo(a)pyrene-induced DNA damage in mice by alkaline single cell gel electrophoresis: evidence for strand breaks in liver but not in lymphocytes and bone marrow. Pharmacology and Toxicology 78 (1996) 37-43