Design-based estimation of neuronal number and individual neuronal volume in the rat hippocampus

Journal of Neuroscience Methods

Volumn 162, Issue 1-2, 2007, Pages 206-214

  Hosseini Sharifabad, Mohammad ^a   Nyengaard, Jens Randel ^b  

^a SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b AARHUS UNIVERSITY   (Denmark)

Author keywords

Deformation; Hippocampus; Number estimation; Size estimation; Stereology; Vertical sections

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CELL COUNT; CELL VOLUME; CONTROLLED STUDY; HIPPOCAMPUS; HISTOLOGY; MALE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RANDOMIZATION; RAT; STEREOLOGY; TECHNIQUE; TISSUE SECTION;

ANIMALS; CROSSES, GENETIC; HIPPOCAMPUS; MALE; MICE; MICE, INBRED BALB C; NEURONS; PYRAMIDAL CELLS; RATS; RATS, LONG-EVANS; RATS, SPRAGUE-DAWLEY; RATS, WISTAR;

EID: 34047258667     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.01.009     Document Type: Article

References (49)

1. Abercrombie M. Estimation of nuclear population from microtome sections. Anat Rec 94 (1946) 239-247
2. Abusaad I., MacKay D., Zhao J., Stanford P., Collier D.A., and Everall I.P. Stereological estimation of the total number of neurons in the murine hippocampus using the optical disector. J Comp Neurol 408 (1999) 60-66
3. Andersen B.B., and Gundersen H.J.G. Pronounced loss of cell nuclei and anisotropic deformation of thick sections. J Microsc 196 (1999) 69-73
4. Baddeley A.J., Gundersen H.J.G., and Cruz-Orive L.M. Estimation of surface area from vertical sections. J Microsc 142 (1986) 259-276
5. Baryshnikova L.M., Von Bohlen Und Halbach O., Kaplan S., and Von Bartheld C.S. Two distinct events, section compression and loss of particles ("lost caps"), contribute to z-axis distortion and bias in optical disector counting. Microsc Res Tech 69 (2006) 738-756
6. 3-thymidine autoradiographic study. Exp Brain Res 46 (1982) 315-323
7. Bonthius D.J., McKim R., Koele L., Harb H., Karacay B., Mahoney J., et al. Use of frozen sections to determine neuronal number in the murine hippocampus and neocortex using the optical disector and optical fractionator. Brain Res Protoc 14 (2004) 45-57
8. Boss B.D., Peterson G.M., and Cowan W.M. On the number of neurons in the dentate gyrus of the rat. Brain Res 338 1 (1985) 144-150
9. Boss B.D., Turlejski K., Stanfield B.B., and Cowan W.M. On the numbers of neurons in fields CA1 and CA3 of the hippocampus of Sprague Dawley and Wistar rats. Brain Res 406 (1987) 280-287
10. Brændgaard H., and Gundersen H.J.G. The impact of recent stereological advances on quantitative studies of nervous system. J Neurosci Meth 18 (1986) 39-78
11. Dorph-Petersen K.A. Stereological estimation using vertical sections in a complex tissue. J Microsc 195 (1999) 79-86
12. Dorph-Petersen K.A., Nyengaard J.R., and Gundersen H.J.G. Tissue shrinkage and unbiased stereological estimation of particle number and size. J Microsc 204 (2001) 232-246
13. Dorph-Petersen K.A., Rosenberg R., and Nyengaard J.R. Estimation of number and volume of immunohistochemically stained neurons in complex brain regions. In: Evans S.M., Janson A.M., and Nyengaard J.R. (Eds). Quantitative Methods in Neuroscience. A Neuroanatomical Approach (2004), Oxford University Press, Oxford 231-232
14. Gardella D., Hatton W.J., Rind H.B., Rosen G.D., and von Bartheld C.S. Differential tissue shrinkage and compression in the z-axis implications for optical disector counting in vibratome, plastic and cryosections. J Neurosci Meth 124 (2003) 45-59
15. Grady M.S., Charleston J.S., Maris D., Witgen B.M., and Lifshitz J. Neuronal and glial cell number in the hippocampus after experimental traumatic brain injury: analysis by stereological estimation. J Neurotrauma 20 10 (2003) 929-941
16. Guillery R.W. On counting and counting errors. J Comp Neurol 447 1 (2002) 1-7
17. Gundersen H.J.G. Notes on the estimation of the numerical density of arbitary profiles: the edge effect. J Microsc 111 (1977) 219-223
18. Gundersen H.J.G. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc 143 (1986) 3-45
19. Gundersen H.J.G. The nucleator. J Microsc 151 (1988) 3-21
20. Gundersen H.J.G., and Jensen E.B. Stereological estimation of the volume-weighted mean volume of arbitrary particles observed on random sections. J Microsc 138 (1985) 127-142
21. Gundersen H.J.G., and Jensen E.B. The efficiency of systematic sampling in stereology and its prediction. J Microsc 147 (1987) 229-263
22. Gundersen H.J.G., Bagger P., Bendtsen T.F., Evans S.M., Korbo L., Marcussen N., et al. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 96 (1988) 857-881
23. Gundersen H.J.G., Jensen E.B., Kieu K., and Nielsen J. The efficiency of systematic sampling in stereology-reconsidered. J Microsc 193 (1999) 199-211
24. Hatton W.J., and von Bartheld C.S. Analysis of cell death in the trochlear nucleus of the chick embryo: calibration of the optical disector counting method reveals systematic bias. J Comp Neurol 409 (1999) 169-186
25. Henrique R.M., Monteiro R.A., Rocha E., and Marini-Abreu M.M. A stereological study on the nuclear volume of cerebellar granule cells in aging rats. Neurobiol Aging 18 (1997) 199-203
26. Iniguez C., Gayoso M.J., and Carreres J. A versatile and simple method for staining nervous tissue using Giemsa dye. J Neurosci Meth 13 (1985) 77-86
27. Jensen E.B., and Gundersen H.J.G. The rotator. J Microsc 170 (1993) 35-44
28. Kempermann G., Kuhn H.G., and Gage F.H. Genetic influence on neurogenesis in the dentate gyrus of adult mice. Proc Natl Acad Sci 94 (1997) 10409-10414
29. Keuker J.I.H., Vollman-Honsdorf G.K., and Fuchs E. How to use the optical fractionator: an example based on the estimation of neurons in the hippocampal CA1 and CA3 regions of tree shrews. Brain Res Protoc 7 (2001) 211-221
30. Korbo L., and Andersen B.B. The distribution of Purkinje cell perikaryon and nuclear volume in human and rat cerebellum with the nucleator method. Neuroscience 69 (1995) 151-158
31. Korbo L., Pakkenberg B., Ladefoged O., Gundersen H.J.G., Arlien-Soborg P., and Pakkenberg H. An efficient method for estimating the total number of neurons in rat brain cortex. J Neurosci Meth 31 (1990) 93-100
32. Ling E.A., Paterson J.A., Privat A., Mori S., and Leblond C.P. Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young rats. J Comp Neurol 149 (1973) 43-72
33. Madeira M.D., Sousa N., Santer R.M., Paula-Barbosa M.M., and Gundersen H.J.G. Age and sex do not affect the volume, cell numbers, or cell size of the suprachiasmatic nucleus of the rat: an unbiased stereological study. J Comp Neurol 361 (1995) 585-601
34. Mattfeldt T., Mall G., Gharehbaghi H., and Muller P. Estimation of surface area and length with the orientator. J Microsc 159 (1990) 301-317
35. Miettinen R.A., Kalesnykas G., and Koivisto E.H. Estimation of the total number of cholinergic neurons containing estrogen receptor-α in the rat basal forebrain. J Histochem Cytochem 50 (2002) 891-902
36. Möller A., Strange P., and Gundersen H.J.G. Efficient estimation of cell volume and number using the nucleator and the disector. J Microsc 159 (1990) 61-71
37. Nyengaard J.R., and Gundersen H.J.G. The isector: a simple and direct method for generating isotropic, uniform random sections from small specimens. J Microsc 165 (1992) 427-431
38. Paxinos G., and Watson C. The rat brain in stereotaxic coordinates (1986), Academic Press
39. Rapp P.R., and Gallagher M. Preserved neuron number in the hippocampus of aged rats with spatial learning deficits. Proc Natl Acad Sci USA 93 (1996) 9926-9930
40. Saper C.B. Any way you cut it: a new journal policy for the use of unbiased counting methods. J Comp Neurol 364 (1996) 5
41. Sousa N., Almeida O.F.X., Holsboer F., Paula-Barbosa M.M., and Madeira M.D. Maintenance of hippocampal cell numbers in young and aged rats submitted to chronic unpredictable stress comparison with the effects of corticosterone treatment. Stress 2 (1998) 237-249
42. Strange P., Moller A., Ladefoged O., Lam H.R., Larsen J.J., and Arlien-Soborg P. Total number and mean cell volume of neocortical neurons in rats exposed to 2,5-hexanedione with and without acetone. Neurotoxicol Teratol 13 (1991) 401-406
43. Weibel E.R., and Gomez D.M. A principle for counting tissue structures on random sections. J Appl Physiol 17 (1962) 343-348
44. West M.J. New stereological methods for counting neurons. Neurobiol aging 14 (1993) 275-285
45. West M.J. Stereological methods for estimating the total number of neurons and synapses: issues of precision and bias. Trends Neurosci 22 (1999) 51-61
46. West M.J., and Andersen A.H. An allometric study of the area dentate in the rat and mouse. Brain Res Rev 2 (1980) 317-348
47. West M.J., and Coleman P.D. How to count. Neurobiol Aging 17 (1996) 503
48. West M.J., Coleman P.D., and Flood D.G. Estimating the number of granule cells in the dentate gyrus with the disector. Brain Res 448 (1988) 167-172
49. West M.J., Slomianka L., and Gundersen H.J.G. Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat Rec 231 (1991) 482-497