Sensitive cell viability assay for use in drug screens and for studying the mechanism of action of drugs in Dictyostelium discoideum

BioTechniques

Volumn 41, Issue 5, 2006, Pages 591-595

  Min, Junxia ^a   Sridevi, Priya ^a   Alexander, Stephen ^a   Alexander, Hannah ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOTOXICITY; DRUG DELIVERY; GENETIC ENGINEERING; LUMINESCENCE;

DICTYOSTELIUM DISCOIDEUM; DRUG DISCOVERY; MUTANT CELLS; MUTANTS;

BIOASSAY;

CISPLATIN;

ARTICLE; CELL VIABILITY; DICTYOSTELIUM DISCOIDEUM; DRUG SCREENING; EVALUATION; GENE MUTATION; HIGH THROUGHPUT SCREENING; LUMINESCENCE; NONHUMAN; STANDARDIZATION; ANIMAL; CELL SURVIVAL; DICTYOSTELIUM; DRUG EFFECT; SENSITIVITY AND SPECIFICITY;

DICTYOSTELIUM DISCOIDEUM;

ANIMALS; CELL SURVIVAL; CISPLATIN; DICTYOSTELIUM; SENSITIVITY AND SPECIFICITY;

EID: 34047232981     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112260     Document Type: Article

References (11)

1. Grove, J.E., R.J. Brown, and D.J. Watts. 2000. The intracellular target for the anti-resorptive aminobisphosphonate drugs in Dictyostelium discoideum is the enzyme farnesyl diphosphate synthase. J. Bone Miner. Res. 15:971-981.
2. Williams, R.S. 2005. Pharmacogenetics in model systems: defining a common mechanism of action for mood stabilisers. Prog. Neuropsychopharmacol. Biol. Psychiatry 29:1029-1037.
3. Alexander, S., J. Min, and H. Alexander. 2006. Dictyostelium discoideum to human cells: pharmacogenetic studies demonstrate a role for sphingolipids in chemoresistance. Biochim. Biophys. Acta 1760:301-309.
4. Li, G., H. Alexander, N. Schneider, and S. Alexander. 2000. Molecular basis for resistance to the anticancer drug cisplatin in Dictyostelium. Microbiology 146:2219-2227.
5. Alexander, H., A.N. Vomund, and S. Alexander. 2003. Viability assay for Dictyostelium for use in drug studies. BioTechniques 35:464-470.
6. Sussman, M. 1987. Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions. Methods Cell Biol. 28:9-29.
7. Min, J., A. Stegner, H. Alexander, and S. Alexander. 2004. Overexpression of sphingosine-1-phosphate lyase or inhibition of sphingosine kinase in Dictyostelium discoideum results in a selective increase in sensitivity to platinum based chemotherapy drugs. Eukaryot. Cell 3:795-805.
8. Min, J., D. Traynor, A.L. Stegner, L. Zhang, M.H. Hanigan, H. Alexander, and S. Alexander. 2005. Sphingosine kinase regulates the sensitivity of Dictyostelium discoideum cells to the anticancer drug cisplatin. Eukaryot. Cell 4:178-189.
9. Deering, R.A. 1988. DNA repair in Dictyostelium. Dev. Genet. 9:483-493.
10. Bronner, C.E., D.L. Welker, and R.A. Deering. 1992. Mutations affecting sensitivity of the cellular slime mold Dictyostelium discoideum to DNA-damaging agents. Mutat. Res. 274:187-200.
11. Kuspa, A. and W.F. Loomis. 1992. Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc. Natl. Acad. Sci. USA 89:8803-8807.