In situ reactions of monoclonal antibodies with a viable mutant of murray valley encephalitis virus reveal an absence of dimeric NS1 protein

Journal of General Virology

Volumn 88, Issue 4, 2007, Pages 1175-1183

  Clark, David C ^a   Lobigs, Mario ^b   Lee, Eva ^b   Howard, Megan J ^c   Clark, Kerri ^a   Blitvich, Bradley J ^c,d   Hall, Roy A ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b Biology and Environment   (Australia)

^c UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^d IOWA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODY; NONSTRUCTURAL PROTEIN 1; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL INVASION; CONTROLLED STUDY; DIMERIZATION; GENETIC SIMILARITY; JAPANESE ENCEPHALITIS VIRUS; KUNJIN VIRUS; MAMMAL CELL; MOUSE; MURRAY VALLEY ENCEPHALITIS VIRUS; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; SAMPLING; SUPERNATANT; VERO CELL; VIROGENESIS; VIRUS ATTENUATION; VIRUS CULTURE; VIRUS MUTANT; VIRUS REPLICATION; VIRUS VIRULENCE; WESTERN BLOTTING; WILD TYPE;

AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; ARTHROPODS; CERCOPITHECUS AETHIOPS; DIMERIZATION; DISEASE MODELS, ANIMAL; ENCEPHALITIS VIRUS, MURRAY VALLEY; ENCEPHALITIS, ARBOVIRUS; FLAVIVIRUS; MICE; MUTAGENESIS, SITE-DIRECTED; VERO CELLS; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

ARTHROPODA; JAPANESE ENCEPHALITIS VIRUS; KUNJIN VIRUS; MAMMALIA; MURRAY VALLEY ENCEPHALITIS VIRUS; MUS;

EID: 34047173550     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/vir.0.82609-0     Document Type: Article

References (33)

1. Arnold, S. J., Osvath, S. R., Hall, R. A., King, N. J. & Sedger, L. M. (2004). Regulation of antigen processing and presentation molecules in West Nile virus-infected human skin fibroblasts. Virology 324, 286-296.
2. Blitvich, B. J., Mackenzie, J. S., Coelen, R. J., Howard, M. J. & Hall, R. A. (1995). A novel complex formed between the flavivirus E and NS1 proteins: analysis of its structure and function. Arch Virol 140, 145-156.
3. Blitvich, B. J., Scanlon, D., Shiell, B. J., Mackenzie, J. S. & Hall, R. A. (1999). Identification and analysis of truncated and elongated species of the flavivirus NS1 protein. Virus Res 60, 67-79.
4. Crooks, A. J., Lee, J. M., Easterbrook, L. M., Timofeev, A. V. & Stephenson, J. R. (1994). The NS1 protein of tick-borne encephalitis virus forms multimeric species upon secretion from the host cell. J Gen Virol 75, 3453-3460.
5. Ellman, G. L. (1959). Tissue sulfhydryl groups. Arch Biochem Biophys 82, 70-77.
6. Fan, W. F. & Mason, P. W. (1990). Membrane association and secretion of the Japanese encephalitis virus NS1 protein from cells expressing NS1 cDNA. Virology 177, 470-476.
7. Flamand, M., Megret, F., Mathieu, M., Lepault, J., Rey, F. A. & Deubel, V. (1999). Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol 73, 6104-6110.
8. Hall, R. A., Burgess, G. W. & Kay, B. H. (1988). Type-specific monoclonal antibodies produced to proteins of Murray Valley encephalitis virus. Immunol Cell Biol 66, 51-56.
9. Hall, R. A., Kay, B. H., Burgess, G. W., Clancy, P. & Fanning, I. D. (1990). Epitope analysis of the envelope and nonstructural glycoproteins of Murray Valley encephalitis virus. J Gen Virol 71, 2923-2930.
10. Hall, R. A., Coelen, R. J. & Mackenzie, J. S. (1991). Immunoaffinity purification of the NS1 protein of Murray Valley encephalitis virus: selection of the appropriate ligand and optimal conditions for elution. J Virol Methods 32, 11-20.
11. Hall, R. A., Brand, T. N., Lobigs, M., Sangster, M. Y., Howard, M. J. & Mackenzie, J. S. (1996). Protective immune responses to the E and NS1 proteins of Murray Valley encephalitis virus in hybrids of flavivirus-resistant mice. J Gen Virol 77, 1287-1294.
12. Hall, R. A., Khromykh, A. A., Mackenzie, J. M., Scherret, J. H., Khromykh, T. I. & Mackenzie, J. S. (1999). Loss of dimerisation of the nonstructural protein NS1 of Kunjin virus delays viral replication and reduces virulence in mice, but still allows secretion of NS1. Virology 264, 66-75.
13. Hall, R. A., Broom, A. K., Smith, D. W. & Mackenzie, J. S. (2002). The ecology and epidemiology of Kunjin virus. Curr Top Microbiol Immunol 267, 253-269.
14. Jacobs, M. G., Robinson, P. J., Bletchly, C., Mackenzie, J. M. & Young, P. R. (2000). Dengue virus nonstructural protein 1 is expressed in a glycosyl-phosphatidylinositol-linked form that is capable of signal transduction. FASEB J 14, 1603-1610.
15. Lee, E. & Lobigs, M. (2000). Substitutions at the putative receptor-binding site of an encephalitic flavivirus alter virulence and host cell tropism and reveal a role for glycosaminoglycans in entry. J Virol 74, 8867-8875.
16. Lee, E. & Lobigs, M. (2002). Mechanism of virulence attenuation of GAG-binding variants of Japanese encephalitis and Murray Valley encephalitis viruses. J Virol 76, 4901-4911.
17. Lee, E., Hall, R. & Lobigs, M. (2004). Common E protein determinants for attenuation of glycosaminoglycan-binding variants of Japanese encephalitis and West Nile viruses. J Virol 78, 8271-8280.
18. Licon Luna, R. M., Lee, E., Müllbacher, A., Blanden, R. V., Langman, R. & Lobigs, M. (2002). Lack of both Fas ligand and perforin protects from flavivirus-mediated encephalitis in mice. J Virol 76, 3202-3211.
19. Lindenbach, B. D. & Rice, C. M. (1997). trans-Complementation of YF virus NS1 reveals a role in early RNA replication. J Virol 71, 9608-9617.
20. Lindenbach, B. D. & Rice, C. M. (2001). Flaviviridae: the viruses and their replication. In Fields Virology, pp. 991-1041. Edited by D. M. Knipe & P. M. Howley. Philadelphia, PA: Lippincott Williams & Wilkins.
21. Lobigs, M., Marshall, I. D., Weir, R. C. & Dalgarno, L. (1988). Murray Valley encephalitis virus field strain from Australian and Papua New Guinea. Studies on the sequence of the major envelope protein gene and virulence for mice. Virology 165, 245-255.
22. Lobigs, M., Müllbacher, A., Wang, Y., Pavy, M. & Lee, E. (2003). Role of type I and type II interferon responses in recovery from infection with an encephalitic flavivirus. J Gen Virol 84, 567-572.
23. Macdonald, J., Tonry, J., Hall, R. A., Williams, B., Palacios, G., Ashok, M. S., Jabado, O., Clark, D., Tesh, R. B. & other authors (2005). NS1 protein secretion during the acute phase of West Nile virus infection. J Virol 79, 13924-13933.
24. Mackenzie, J. S., Lindsay, M. D., Coelen, R. J., Broom, A. K., Hall, R. A. & Smith, D. W. (1994). Arboviruses causing human disease in the Australasian zoogeographic region. Arch Virol 136, 447-467.
25. Mackenzie, J. M., Jones, M. K. & Young, P. R. (1996). Immunolocalization of the dengue viral nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology 220, 232-240.
26. Mackenzie, J. S., Johansen, C. A., Ritchie, S. A., van den Hurk, A. F. & Hall, R. A. (2002). Japanese encephalitis as an emerging virus: the emergence and spread of Japanese encephalitis virus in Australasia. Curr Top Microbiol Immunol 267, 49-73.
27. Mason, P. W. (1989). Maturation of Japanese encephalitis virus glycoproteins produced by infected mammalian and mosquito cells. Virology 169, 354-364.
28. May, F. J., Lobigs, M., Lee, E., Gendle, D. J., Mackenzie, J. S., Broom, A. K., Conlan, J. V. & Hall, R. A. (2006). Biological, antigenic and phylogenetic characterization of the flavivirus Alfuy. J Gen Virol 87, 329-337.
29. Müller, U., Steinhoff, U., Reis, L. F., Hemmi, S., Pavlovic, J., Zinkernagel, R. M. & Aguet, M. (1994). Functional role of type I and type II interferons in antiviral defense. Science 264, 1918-1921.
30. Muylaert, I. R., Galler, R. & Rice, C. M. (1997). Genetic analysis of the yellow fever virus NS1 protein: identification of a temperature sensitive mutation which blocks RNA accumulation. J Virol 71, 291-298.
31. Schlesinger, J. J., Brandriss, M. W. & Walsh, E. E. (1985). Protection against 17D yellow fever encephalitis in mice by passive transfer of monoclonal antibodies to the nonstructural glycoprotein gp48 and by active immunization with gp48. J Immunol 135, 2805-2809.
32. Schlesinger, J. J., Brandriss, M. W., Cropp, C. B. & Monath, T. P. (1986). Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural protein NS1. J Virol 60, 1153-1155.
33. Winkler, G., Maxwell, S. E., Ruemmler, C. & Stollar, V. (1989). Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization. Virology 171, 302-305.