Synthetically lethal interactions involving loss of the yeast ERG24: The sterol C-14 reductase gene

Lipids

Volumn 42, Issue 1, 2007, Pages 69-76

  Bhuiyan, M Shah Alam ^a   Eckstein, James ^b   Barbuch, Robert ^b   Bard, Martin ^a  

^a INDIANA UNIVERSITY   (United States)

^b ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DELETION CONSORTIUM BACKGROUND; DOUBLE MUTANTS; MORPHOLINE ANTIFUNGAL COMPOUNDS; REDUCTASE GENES;

ALCOHOLS; ENZYMES; GENES; GENETIC ENGINEERING; LIPIDS; MUTAGENESIS; SYNTHESIS (CHEMICAL);

YEAST;

C 8 STEROL ISOMERASE; CALCIUM CHLORIDE; ISOMERASE; OXIDOREDUCTASE; SPHINGOLIPID; STEROL; STEROL C 14 REDUCTASE; UNCLASSIFIED DRUG;

CELL VIABILITY; CONFERENCE PAPER; CULTURE MEDIUM; ELO2 GENE; ELO3 GENE; ERG2 GENE; ERG24 GENE; FUNGAL GENE; FUNGAL GENETICS; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS MUTANT; GENE; GENE DELETION; GENE REPRESSION; HAPLOIDY; LETHALITY; NONHUMAN; POLYMERASE CHAIN REACTION; YEAST;

GENE DELETION; GENES, FUNGAL; OXIDOREDUCTASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; STEROLS;

EID: 33947639577     PISSN: 00244201     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11745-006-1001-4     Document Type: Conference Paper

References (17)

1. Lorenz RT, Parks LW (1992) Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae. DNA Cell Biol 11:685-692
2. Marcireau C, Guyonnet D, Karst F (1992) Construction and growth properties of a yeast strain defective in sterol 14-reductase. Curr Genet 22:267-272
3. Oh CS, Toke DA, Mandala S, Martin CE (1997) ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272:17376-17384
4. Mercer EI (1993) Inhibitors of sterol biosynthesis and their applications. Prog Lipid Res 32:357-416
5. 14 reductase by fenpropiomorph, tridemorph, and fenpropidin in cell-free enzyme systems from Saccharomyces cerevisiae. Phytochem 26:663-668
6. Ladeveze V, Marcireau C, Delourme D, Karst F (1993) General resistance to sterol biosynthesis inhibitors in Saccharomyces cerevisiae. Lipids 28:907-912
7. Baudry K, Swain E, Rahier A, Germann M, Batta A, Rondet S, Mandala S, Henry K, Tint GS, Edlind T, Kurtz M, Nickels JT (2001) The effect of the Erg26-l mutation on the regulation of lipid metabolism in Saccharomyces cerevisiae. J Biol Chem 276:12702-12711
8. Crowley JH, Smith SJ, Leak FW, Parks LW (1996) Aerobic isolation of an ERG24 null mutant of Saccharomyces cerevisiae. J Bacteriol 178:2991-2993
9. Crowley JH, Tove S, Parks LW (1998) A calcium-dependent ergosterol mutant of Saccharomyces cerevisiae. Curr Genet 34:93-99
10. Lai MH, Bard M, Pierson CA, Alexander JF, Goebl M, Carter GT, Kirsch DR (1994) The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 11:41-49
11. Silve S, Leplatois P, Josse A, Dupuy PH, Lanau C, Kaghad M, Dhers C, Picard C, Rahier A, Taton M, Le Fur G, Caput D, Ferrara P, Loison G (1996) The immunosuppressant SR 31747 blocks cell proliferation by inhibiting a steroid isomerase in Saccharomyces cerevisiae. Mol Cell Biol 16:2719-2727
12. Winzeler EA et al (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 6:901-906
13. Valachovic M, Bareither BM, Bhuiyan MSA, Eckstein J, Barbuch R, Balderes D, Wilcox L, Sturley SL, Dickson RC, Bard M (2006) Cumulative mutations affecting sterol biosynthesis in the yeast Saccharomyces cerevisiae result in synthetic lethality that is suppressed by alterations in sphingolipid profiles. Genetics 173:1893-1908
14. Adams A, Gottschling DE, Kaiser CA, Stearns T (1997) Methods in yeast genetics. Cold Spring Harbor Laboratory Press, New York
15. Sikorski RS, Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27
16. Gachotte D, Sen SE, Eckstein J, Barbuch R, Krieger M, Ray BD, Bard M (1999) Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation. Proc Natl Acad Sci USA 26:12655-12660
17. Eisenkolb M, Zenzmaier C, Leitner E, Schneller R (2002) A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast. Mol Biol Cell 13:4414-4428