Molecular cloning and characterization of one member of 3β-hydroxy sterol glucosyltransferase gene family in Withania somnifera

Archives of Biochemistry and Biophysics

Volumn 460, Issue 1, 2007, Pages 48-55

  Sharma, Lokendra Kumar ^a   Madina, Bhaskara Reddy ^a   Chaturvedi, Pankaj ^a   Sangwan, Rajender Singh ^a   Tuli, Rakesh ^a  

^a CSIR NATIONAL BOTANICAL RESEARCH INSTITUTE   (India)

Author keywords

Heat shock; Salicylic acid; Secondary metabolism; Sterol glucosyltransferase; Substrate specificity; UGTs; Withania somnifera

Indexed keywords

3BETA HYDROXYSTEROL GLUCOSYLTRANSFERASE; GLUCOSYLTRANSFERASE; SALICYLIC ACID; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLANT LEAF; PRIORITY JOURNAL; UNTRANSLATED REGION; WITHANIA SOMNIFERA;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENES, PLANT; GLUCOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; PLANT LEAVES; PLANT PROTEINS; SEQUENCE ALIGNMENT; TIME FACTORS; WITHANIA;

ARABIDOPSIS THALIANA; ESCHERICHIA COLI; WITHANIA SOMNIFERA;

EID: 33947593377     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2007.01.024     Document Type: Article

References (42)

1. Bowles D., Lim E.K., Poppenberger B., and Vaistij F.E. Glycosyltransferases of lipophilic small molecules. Annu. Rev. Plant Biol. 57 (2006) 567-597
2. Sandermann H., and Pflugmacher S. Taxonomic distribution of plant glucosyl transferases acting on xenobiotics. Phytochemistry 49 (1998) 507-511
3. Ikan R. Naturally Occurring Glycosides (1999), Wiley, Chichester, UK
4. Radominska-Pandya A., Czernik P.J., Little J.M., Battaglia E., and Mackenzie P.I. Structural and functional studies of UDP-glucuronosyltransferases. Drug Metab. Rev. 31 (1999) 817-899
5. Vogt T., and Jones P. Glycosyltransferases in plant natural product synthesis: characterization of a supergene family. Trends Plant Sci. 5 (2000) 380-386
6. Jones P., and Vogt T. Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers. Planta 213 (2001) 164-174
7. Paquette S., Moller B.L., and Bak S. On the origin of family 1 plant glycosyltransferases. Phytochemistry 62 (2003) 399-413
8. Milkowski C., Baumert A., and Strack D. Identification of four Arabidopsis genes encoding hydroxycinnamate glucosyltransferases. FEBS Lett. 486 (2000) 183-184
9. Jackson R.G., Lim E.K., Li Y., Kowalczyk M., Sandberg G., Hoggett J., Ashford D.A., and Bowles D.J. Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase. J. Biol. Chem. 276 (2001) 4350-4356
10. Lim E.K., Li Y., Parr A., Jackson R., Ashford D.A., and Bowles D.J. Identification of glucosyltransferase genes involved in sinapate metabolism and lignin synthesis in Arabidopsis. J. Biol. Chem. 276 (2001) 4344-4349
11. Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., and Bowles D.J. The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates. J. Biol. Chem. 277 (2002) 586-592
12. Jones P., Messner B., Nakajima J., Schaffner A.R., and Saito K. Glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana. J. Biol. Chem. 278 (2003) 43910-43918
13. Osbourn A.E. Saponins and plant defense a soap story. Trends Plant Sci. 1 (1996) 4-9
14. Armah C.N., Mackie A.R., Roy C., Price K., Osourn A.E., Bowyer P., and Ladha S. The membrane permeabilising effect of avenacin A-1 involves the reorganization of bilayer cholesterol. Biophys. J. 76 (1999) 281-290
15. Arneson P.A., and Durbin R.D. Studies on the mode of action of tomatine as a fungitoxic agent. Plant Physiol. 43 (1968) 683-686
16. Duncan E.A., Dave U.P., Sakai J., Goldstein J.L., and Brown M.S. Second-site cleavage in sterol regulatory element-binding protein occurs at transmembrane junction as determined by cysteine panning. J. Biol. Chem. 273 (1998) 17801-17809
17. Bach T.J., and Benveniste P. Cloning of cDNAs or genes encoding enzymes of sterol biosynthesis from plants and other eukaryotes: heterologous expression and complementation analysis of mutations for functional characterization. Prog. Lipid Res. 36 (1997) 197-226
18. Leber R., Landl K., Zinser E., Ahorn H., Spok A., Kohlwein S.D., Turnowsky F., and Daum G. Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol. Biol. Cell 9 (1998) 375-386
19. Dewick P.M. Medicinal Natural Product: A Biosynthetic Approach. second ed. (2001), Wiley, New York 237-241
20. Rohmer M. The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants. Nat. Prod. Rep. 16 (1999) 565-574
21. Singh B., Saxena A.K., Chandan B.K., Gupta D.K., Bhutani K.K., and Anand K.K. Adaptogenic activity of a novel, withanolide-free aqueous fraction from the roots of Withania somnifera. Dun. Phytother. Res. 15 (2001) 311-318
22. Mishra L., Lal P., Sangwan R.S., Sangwan N.S., Uniyal G.C., and Tuli R. Unusually sulfated and oxygenated steroids from Withania somnifera. Phytochemistry 66 (2005) 2702-2707
23. Lal P., Mishra L., Sangwan R.S., and Tuli R. New withanolides from fresh berries of Withania somnifera. Z. Naturforsch. 61b (2006) 1143-1147
24. Matsuda H., Murakami T., Kishi A., and Yoshikawa M. Structures of withanosides I, II, III, IV, V, VI, and VII, new withanolide glycosides, from the roots of Indian Withania somnifera DUNAL and inhibitory activity for tachyphylaxis to clonidine in isolated guinea-pig ileum. Bioorg. Med. Chem. 9 (2001) 1499-1507
25. Pawlowski K., Kunze R., Vries S.D., and Bisseling T. Isolation of total poly (A) and polysomal RNA from plant tissue. Plant Mol. Biol. Manual D5 (1994) 1-13
26. Warnecke D., Erdmann R., Fahl A., Hube B., Muller F., Zank T., Zahringer U., and Heinz E. Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum. J. Biol. Chem. 274 (1999) 13048-13059
27. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbour Lab. Press, Cold Spring Harbour, NY
28. Warnecke D.C., Baltrusch M., Buck F., Wolter F.P., and Heinz E. UDP-glucose: sterol glucosyltransferase: cloning and functional expression in Escherichia coli. Plant Mol. Biol. 35 (1997) 597-603
29. Tmethod. Methods 25 (2001) 402-408
30. Ullmann P., Ury A., Rimmele D., Benveniste P., and Bouvier-Nave P. UDP-glucose sterol beta-D-glucosyltransferase, a plasma membrane-bound enzyme of plants: enzymatic properties and lipid dependence. Biochimie 75 (1993) 713-723
31. Verhock B., Haas R., Wrage K., Linscheid M., and Heinz E. Lipids and enzymatic activity in vacuoler membranes isolated via protoplasts from oat primary leaves. Z. Naturforsch. 38 (1983) 770-777
32. Moehs C.P., Allen P.V., and Belknep W.R. Cloning and expression of Solanidine UDP-glucose glucosyltransferase from potato. Plant J. 11 (1997) 227-236
33. Kohara A., Nakajima C., Hashimoto K., Ikenaza T., Tanaka H., Shoyama Y., Yoshida S., and Muranaka T. A novel glucosyltransferase involved in saponin biosynthesis in Solanum aculeatissinum. Plant Mol. Biol. 57 (2005) 225-239
34. Lee H., Kobayashi M., Wang P., Nakayama J., Seeberger P.H., and Fukuda M. Expression cloning of cholesterol α-glucosyltransferase, a unique enzyme that can be inhibited by natural antibiotic gastric mucin O-glycans from Helicobacter pylori. Biochem. Biophys. Res. Commun. 349 (2006) 1235-1241
35. Kubo A., Arai Y., Nagashima S., and Yoshikawa T. Alteration of sugar donor specificity of plant glycosyltransferase by a single point mutation. Arch. Biochem. Biophys. 429 (2004) 198-203
36. Jayaprakasam B., and Nair M.G. Cyclooxygenase-2 enzyme inhibitory withanolide from Withania somnifera leaves. Tetrahedron 59 (2003) 841-849
37. Ward E.R., Uknes S.J., Williams S.C., Dincher S.S., Wiederhold D.L., Alexander D.C., Ahl-Goy P., Metraux J.P., and Ryals J.A. Coordinate gene activity in response to agents that induce systemic acquired resistance. Plant Cell 3 (1991) 1085-1094
38. Hlywka J.J., Stephenson G.R., Sears M.K., and Yada R.Y. Effect of insect damage on glycoalkaloid content in potato (Solanum tuberosum). J. Agric. Food Chem. 42 (1994) 2545-2550
39. Keukens E.A.J., Vrije T.D., Boom C.V.D., Waard P.D., Plasmna H.H., Theil F., Chupin V., Jongen W.M.F., and Kruijff B.D. Molecular basis of glycoalkaloid induced membrane disruption. Biochim. Biophys. Acta 1240 (1995) 216-228
40. Murakami-Murofushi K., Nishikawa K., Hirakawa E., and Murofushi H. Heat stress induces a glycosylation of membrane sterol in myxoamoebae of a true slime mold, Physarum polycephalum. J. Biol. Chem. 272 (1997) 486-489
41. Kunimoto S., Murofushi W., Kai H., Ishida Y., Uchiyama A., Kobayashi T., Kobayashi S., Murofushi H., and Murakami-Murofushi K. Steryl glucoside is a lipid mediator in stress responsive signal transduction. Cell Struct. Funct. 27 (2002) 157-162
42. Glombitza S., Dubuis P.H., Thulke O., and Welzl G. Crosstalk and differential response to abiotic and biotic stresses reflected at the transcriptional level of effector genes from secondary metabolism. Plant Mol. Biol. 54 (2004) 817-835