Engineering of stable recombinant bacteria for production of chiral medium-chain-length poly-3-hydroxyalkanoates

Applied and Environmental Microbiology

Volumn 65, Issue 8, 1999, Pages 3265-3271

  Prieto, Maria A ^a,b   Kellerhals, Michele B ^a   Bozzato, Gian B ^a   Radnovic, Dragan ^a,c   Witholt, Bernard ^a   Kessler, Birgit ^a  

^a ETH ZURICH   (Switzerland)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^c UNIVERSITY OF NOVI SAD   (Serbia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; DNA POLYMERASE; POLYHYDROXYALKANOIC ACID;

ARTICLE; CHIRALITY; ESCHERICHIA COLI; FERMENTATION; GENETIC ENGINEERING; GRAM NEGATIVE BACTERIUM; NONHUMAN; PHENOTYPE; PSEUDOMONAS OLEOVORANS; TRANSPOSON;

ESCHERICHIA COLI; PSEUDOMONAS OLEOVORANS;

EID: 0032771985     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.8.3265-3271.1999     Document Type: Article

References (42)

1. Anderson, A. J., and E. A. Dawes. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54:450-472.
2. Bagdasarian, M., R. Lurz, B. Ruckert, F. C. H. Franklin, M. M. Bagdasarian, J. Frey, and K. N. Timmis. 1981. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors for gene cloning in Pseudomonas. Gene 16:237-247.
3. Black, P. N., and C. C. DiRusso. 1994. Molecular and biochemical analyses of fatty acid transport, metabolism, and gene regulation in Escherichia coli. Biochim. Biophys. Acta 1210:123-145.
4. Chen, C.-Y., C. Stephen, and C. Winass. 1991. Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter. J. Bacteriol. 173:1139-1144.
5. de Koning, G. J. M., M. B. Kellerhals, C. van Meurs, and B. Witholt. 1996. Poly(hydroxyalkanoates) from fluorescent pseudomonads in retrospect and prospect. J. Environ. Polymer Deg. 4:243-252.
6. de Lorenzo, V., S. Fernandez, M. Herrero, U. Jakubzik, and K. N. Timmis. 1993. Engineering of alkyl- and haloaromatic-responsive gene expression with mini-transposons containing regulated promoters of biodegradative pathways of Pseudomonas. Gene 130:41-46.
7. de Lorenzo, V., and K. N. Timmis. 1994. Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods Enzymol. 235:386-405.
8. de Smet, M. J., G. Eggink, B. Witholt, J. Kingma, and H. Wynberg. 1983. Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane. J. Bacteriol. 154:870-878.
9. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of Escherichia coli by high voltage electroporation. Nucleic Acids Res. 16:6127.
10. Fidler, S., and D. Dennis. 1992. Polyhydroxyalkanoate production in recombinant Escherichia coli. FEMS Microbiol. Rev. 103:231-236.
11. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmid. J. Mol. Biol. 166:557-580.
12. Hazenberg, W. M. 1997. Production of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans in two-liquid-phase media. Ph.D. thesis. ETH, Zürich, Switzerland.
13. Herrero, M., V. de Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic selection markers for cloning and stable chromosomal insertion of foreign DNA in gram-negative bacteria. J. Bacteriol. 172:6557-6567.
14. Huijberts, G. N. M., G. Eggink, P. de Waard, G. W. Huisman, and B. Witholt. 1992. Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Appl. Environ. Microbiol. 58:536-544.
15. 13C nuclear magnetic resonance studies of Pseudomonas putida fatty acid metabolic routes involved in poly(3-hydroxyalkanoate) synthesis. J. Bacteriol. 176:1661-1666.
16. Huisman, G. W., O. de Leeuw, G. Eggink, and B. Witholt. 1989. Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. Appl. Environ. Microbiol. 55:1949-1954.
17. Huisman, G. W., E. Wonink, R. Meima, B. Kazemier, P. Terpstra, and B. Witholt. 1991. Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. J. Biol. Chem. 266:2191-2198.
18. Huisman, G. W., E. Wonink, G. J. M. de Koning, H. Preusting, and B. Witholt. 1992. Synthesis of poly(3-hydroxyalkanoates) by mutant and recombinant Pseudomonas strains. Appl. Microbiol. Biotechnol. 38:1-5.
19. Kraak, M. N., T. H. M. Smits, B. Kessler, and B. Witholt. 1997. Polymerase C1 levels and poly(R-3-hydroxyalkanoate) synthesis in wild-type and recombinant Pseudomonas strains. J. Bacteriol. 179:4985-4991.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
21. Lageveen, R. G., G. W. Huisman, H. Preusting, P. Ketelaar, G. Eggink, and B. Witholt. 1988. Formation of polyesters by Pseudomonas oleovorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Appl. Environ. Microbiol. 54:2924-2932.
22. Langenbach, S., B. H. A. Rehm, and A. Steinbüchel. 1997. Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate) synthesis. FEMS Microbiol. Lett. 150:303-309.
23. Lee, E. Y., and C. Y. Choi. 1995. Gas chromatography-mass spectrometric analysis and its application to a screening procedure for novel bacterial polyhydroxyalkanoic acids containing long chain saturated and unsaturated monomers. J. Ferment. Bioeng. 80:408-414.
24. Magnuson, K., S. Jackowski, C. O. Rock, and J. E. Cronan. 1993. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57:522-542.
25. Ohashi, T., and J. Hasegawa. 1992. D(-)-β-Hydroxycarboxylic acids as raw materials for captopril and β-lactams, p. 269-278. In A. N. Collins, G. N. Sheldrake, and J. Crosby (ed.), Chirality in industry. ZENECA Specialities, Manchester, United Kingdom.
26. Page, W. J. 1995. Bacterial polyhydroxyalkanoates, natural biodegradable plastics with a great future. Can. J. Microbiol. 41:1-3.
27. Poirier, Y., D. E. Dennis, K. Klomparens, and C. Sommerville. 1992. Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. Science 256:520-523.
28. Preusting, H., A. Nijenhuis, and B. Witholt. 1990. Physical characteristics of poly(3-hydroxyalkanoates) and poly(3-hydroxyalkenoates) produced by Pseudomonas oleovorans grown on aliphatic hydrocarbons. Macromolecules 23:4220-4224.
29. Qi, Q., B. H. A. Rehm, and A. Steinbüchel. 1997. Synthesis of poly(3-hydroxyalkanoate) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2. FEMS Microbiol. Lett. 157:155-162.
30. Qi, Q., A. Steinbüchel, and B. H. A. Rehm. 1998. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): inhibition of fatty acid β-oxidation by acrylic acid. FEMS Microbiol. Lett. 167:89-94.
31. Ramos, J. L., N. Mermod, and K. N. Timmis. 1987. Regulatory circuits controlling transcription of TOL plasmid operon encoding meta-cleavage pathway for degradation of alkylbenzoates by Pseudomonas. Mol. Microbiol. 1:293-300.
32. Ren, Q. 1997. Biosynthesis of medium chain length poly-3-hydroxyalkanoates: from Pseudomonas to Escherichia coli. Ph.D. thesis. ETH, Zürich, Switzerland.
33. + Escherichia coli. Appl. Environ. Microbiol. 61:2487-2492.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Schaad, N. W. 1988. Laboratory guide for identification of plant pathogenic bacteria. American Phytopathological Society, St. Paul, Minn.
36. Schwartz, R. D., and C. J. McCoy. 1973. Pseudomonas oleovorans hydroxylation-epoxidation system: additional strain improvements. Appl. Microbiol. 26:217-218.
37. Sim, S. J., K. D. Snell, S. A. Hogan, J. Stubbe, C. Rha, and A. J. Sinskey. 1997. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nat. Biotechnol. 15:63-67.
38. Slater, S., T. Gallaher, and D. Dennis. 1992. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain. Appl. Environ. Microbiol. 58:1089-1094.
39. Steinbüchel, A., E. Hustede, M. Liebergesell, U. Pieper, A. Timm, and H. Valentin. 1992. Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. FEMS Microbiol. Rev. 103:217-230.
40. Steinbüchel, A., and H. E. Valentin. 1995. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol. Lett. 128:219-228.
41. van der Leij, F. R., and B. Witholt. 1995. Strategies for the sustainable production of new biodegradable polyesters in plants: a review. Can. J. Microbiol. 41:222-238.
42. Wubbolts, M. G., O. Favre-Bulle, and B. Witholt. 1996. Biosynthesis of synthons in two-liquid-phase media. Biotechnol. Bioeng. 52:301-308.