Application of flow cytometry for the determination of minimal inhibitory concentration of several antibacterial agents on Mycoplasma hyopneumoniae

Journal of Applied Microbiology

Volumn 102, Issue 4, 2007, Pages 1132-1137

  Assuncao P ^a   Antunes, N T ^a   Rosales, R S ^a   Poveda, C ^a   De La Fe, C ^a   Poveda, J B ^a   Davey, H M ^b  

^a UNIVERSITY OF LAS PALMAS DE GRAN CANARIA   (Spain)

^b ABERYSTWYTH UNIVERSITY   (United Kingdom)

Author keywords

Antibiotics; Flow cytometry; Minimal inhibitory concentrations; Mycoplasma hyopneumoniae; Susceptibility

Indexed keywords

ANTIBIOTICS; MICROWAVE INTEGRATED CIRCUITS;

CONCENTRATION VALUES; GENTAMYCIN; GOOD METHODS; MINIMAL INHIBITORY CONCENTRATION; MYCOPLASMA HYOPNEUMONIAE; NEAR-REAL TIME; SPECTINOMYCIN; SUSCEPTIBILITY; TYLOSIN;

FLOW CYTOMETRY;

ANTIINFECTIVE AGENT; CHLORAMPHENICOL; CIPROFLOXACIN; ENROFLOXACIN; GENTAMICIN; LINCOMYCIN; OXYTETRACYCLINE; SPECTINOMYCIN; STREPTOMYCIN; TYLOSIN;

ANTIBIOTIC RESISTANCE; ANTIMICROBIAL ACTIVITY; BACTERIUM; DETECTION METHOD; FLOW CYTOMETRY; INHIBITION; INHIBITOR; SUBPOPULATION;

ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; COLOR; CULTURE MEDIUM; FLOW CYTOMETRY; INCUBATION TIME; INTERMETHOD COMPARISON; MINIMUM INHIBITORY CONCENTRATION; MYCOPLASMA HYOPNEUMONIAE; NONHUMAN;

MYCOPLASMA HYOPNEUMONIAE;

EID: 33947418442     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2006.03170.x     Document Type: Article

References (29)

1. Alvarez-Barrientos, A., Arroyo, J., Canton, R., Nombela, C. and Sanchez-Perez, M. (2000) Applications of flow cytometry to clinical microbiology. Clin Microbiol Rev 13, 167-195.
2. Assunção, P., Rosales, R.S., Rifatbegovic, M., Antunes, N.T., de la Fe, C., Ruiz de Galarreta, C.M. and Poveda, J.B. (2006) Quantification of mycoplasmas in broth medium with sybr green-I and flow cytometry. Front Biosci 11, 492-497.
3. Braga, P.C., Bovio, C., Culici, M. and Dal Sasso, M. (2003) Flow cytometric assessment of susceptibilities of Streptococcus pyogenes to erythromycin and rokitamycin. Antimicrob Agents Chemother 47, 408-412.
4. Davey, H.M. and Kell, D.B. (1996) Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol Rev 60, 641-696.
5. Davey, H.M. and Winson, M.K. (2003) Using flow cytometry to quantify microbial heterogeneity. Curr Issues Mol Biol 5, 9-15.
6. Friis, N.F. (1975) Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey. Nord Vet Med 27, 337-339.
7. Friis, N.F. and Szancer, J. (1994) Sensitivity of certain porcine and bovine mycoplasmas to antimicrobial agents in a liquid medium test compared to a disc assay. Acta Vet Scand 35, 389-394.
8. Haesebrouck, F., Pasmans, F., Chiers, K., Maes, D., Ducatelle, R. and Decostere, A. (2004) Efficacy of vaccines against bacterial diseases in swine: what can we expect? Vet Microbiol 100, 255-268.
9. Hannan, P.C. (2000) Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species. International Research Programme on Comparative Mycoplasmology. Vet Res 31, 373-395.
10. Hannan, P.C., Windsor, H.M. and Ripley, P.H. (1997) In vitro susceptibilities of recent field isolates of Mycoplasma hyopneumoniae and Mycoplasma hyosynoviae to valnemulin (Econor), tiamulin and enrofloxacin and the in vitro development of resistance to certain antimicrobial agents in Mycoplasma hyopneumoniae. Res Vet Sci 63, 157-160.
11. Hannan, P.C.T., O'Hanlon, P.J. and Rogers, N.H. (1989) In vitro evaluation of various quinolone antibacterial agents against veterinary mycoplasmas and porcine respiratory bacterial pathogens. Res Vet Sci 46, 202-211.
12. Kobisch, M. and Friis, N.F. (1996) Swine mycoplasmoses. Rev Sci Tech 15, 1569-1605.
13. Mason, D.J., Power, E.G., Talsania, H., Phillips, I. and Gant, V.A. (1995) Antibacterial action of ciprofloxacin. Antimicrob Agents Chemother 39, 2752-2758.
14. Mortimer, F.C., Mason, D.J. and Gant, V.A. (2000) Flow cytometric monitoring of antibiotic-induced injury in Escherichia coli using cell-impermeant fluorescent probes. Antimicrob Agents Chemother 44, 676-681.
15. Novo, D.J., Perlmutter, N.G., Hunt, R.H. and Shapiro, H.M. (2000) Multiparameter flow cytometric analysis of antibiotic effects on membrane potential, membrane permeability, and bacterial counts of Staphylococcus aureus and Micrococcus luteus. Antimicrob Agents Chemother 44, 827-834.
16. Roth, B.L., Poot, M., Yue, S.T. and Millard, P.J. (1997) Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl Environ Microbiol 63, 2421-2431.
17. Sárkösy, G. (2001) Quinolones: a class of antimicrobial agents. Vet Med Czech 46, 257-274.
18. Shapiro, H.M. (2004) Practical Flow Cytometry, 4th edn. NJ: Jonh Wiley & Sons, Inc.
19. Stakenborg, T., Vicca, J., Butaye, P., Maes, D., Minion, F.C., Peeters, J, De Kruif, A. and Haesebrouck, F. (2005) Characterization of in vivo acquired resistance of Mycoplasma hyopneumoniae to macrolides and lincosamides. Microb Drug Resist 11, 290-294.
20. Suller, M.T., Stark, J.M. and Lloyd, D. (1997) A flow cytometric study of antibiotic-induced damage and evaluation as a rapid antibiotic susceptibility test for methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 40, 77-83.
21. Tanner, A.C. and Wu, C.C. (1992) Adaptation of the sensititre broth microdilution technique to antimicrobial susceptibility testing of Mycoplasma gallisepticum. Avian Dis 36, 714-717.
22. Tanner, A.C., Erickson, B.Z. and Ross, R.F. (1993) Adaptation of the sensititre broth microdilution technique to antimicrobial susceptibility testing of Mycoplasma hyopneumoniae. Vet Microbiol 36, 301-306.
23. Ter Laak, E.A., Pijpers, A., Noordergraaf, J.H., Schoevers, E.C. and Verheijden, J.H. (1991) Comparison of methods for in vitro testing of susceptibility of porcine Mycoplasma species to antimicrobial agents. Antimicrob Agents Chemother 35, 228-233.
24. Vicca, J., Stakenborg, T., Maes, D., Butaye, P., Peeters, J., de Kruif, A. and Haesebrouck, F. (2004) In vitro susceptibilities of Mycoplasma hyopneumoniae field isolates. Antimicrob Agents Chemother 48, 4470-4472.
25. Walberg, M. and Steen, H.B. (2001) Flow cytometric monitoring of bacterial susceptibility to antibiotics. Methods Cell Biol 64, 553-566.
26. Walberg, M., Gaustad, P. and Steen, H.B. (1996) Rapid flow cytometric assessment of mecillinam and ampicillin bacterial susceptibility. J Antimicrob Chemother 37, 1063-1075.
27. Walberg, M., Gaustad, P. and Steen, H.B. (1997a) Rapid discrimination of bacterial species with different ampicillin susceptibility levels by means of flow cytometry. Cytometry 29, 267-272.
28. Walberg, M., Gaustad, P. and Steen, H.B. (1997b) Rapid assessment of ceftazidime, ciprofloxacin, and gentamicin susceptibility in exponentially-growing E. coli cells by means of flow cytometry. Cytometry 27, 169-178.
29. Wickens, H.J., Pinney, R.J., Mason, D.J. and Gant, V.A. (2000) Flow cytometric investigation of filamentation, membrane patency, and membrane potential in Escherichia coli following ciprofloxacin exposure. Antimicrob Agents Chemother 44, 682-687.