Identification of a sterol Δ7 reductase gene involved in desmosterol biosynthesis in Mortierella alpina 1S-4

Applied and Environmental Microbiology

Volumn 73, Issue 6, 2007, Pages 1736-1741

  Zhang, Shuo ^a   Sakuradani, Eiji ^a   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOLS; AMINO ACIDS; BIOSYNTHESIS; CLONING; ENZYMES; YEAST;

DESMOSTEROLS; MOLECULAR CLONING;

GENE ENCODING;

CAMPESTEROL; DESMOSTEROL; 22,23 DIHYDROERGOSTEROL; 22,23-DIHYDROERGOSTEROL; 7 DEHYDROCHOLESTEROL REDUCTASE; 7-DEHYDROCHOLESTEROL REDUCTASE; CHOLESTA 5,7,24 TRIEN 3 BETA OL; CHOLESTA-5,7,24-TRIEN-3 BETA-OL; CHOLESTEROL; COLECALCIFEROL; DRUG DERIVATIVE; ERGOSTEROL; FUNGAL DNA; OXIDOREDUCTASE; PHYTOSTEROL; STEROL; UNCLASSIFIED DRUG;

AMINO ACID; ENZYME ACTIVITY; FUNGUS; GENETIC ANALYSIS; GENETIC ENGINEERING; IDENTIFICATION METHOD; RNA; STEROL;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; FUNGAL GENE; GENE EXPRESSION; GENE IDENTIFICATION; MOLECULAR CLONING; MORTIERELLA ALPINA; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; SACCHAROMYCES CEREVISIAE; XENOPUS LAEVIS; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENE SILENCING; GENETICS; ISOLATION AND PURIFICATION; MASS FRAGMENTOGRAPHY; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; MORTIERELLA; SEQUENCE HOMOLOGY;

FUNGI; MORTIERELLA ALPINA; SACCHAROMYCES CEREVISIAE; XENOPUS LAEVIS;

ANIMALS; BASE SEQUENCE; CHOLECALCIFEROL; CHOLESTEROL; CLONING, MOLECULAR; DESMOSTEROL; DNA, FUNGAL; ERGOSTEROL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENE EXPRESSION; GENE SILENCING; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MOLECULAR WEIGHT; MORTIERELLA; OPEN READING FRAMES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; PHYTOSTEROLS; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID; STEROLS; XENOPUS LAEVIS;

EID: 33947408774     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02425-06     Document Type: Article

References (30)

1. Bae, S. H., J. N. Lee, B. U. Fitzky, J. Seong, and Y. K. Paik. 1999. Cholesterol biosynthesis from lanosterol: molecular cloning, tissue distribution, expression, chromosomal localization, and regulation of rat 7-dehydrocholesterol reductase, a Smith-Lemli-Opitz syndrome-related protein. J. Biol. Chem. 274:14624-14631.
2. Delorme, E. 1989. Transformation of Saccharomyces cerevisiae by electroporation. Appl. Environ. Microbiol. 55:2242-2246.
3. Duport, C., R. Spagnoli, E. Degryse, and D. Pompon. 1998. Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast. Nat. Biotechnol. 16:186-189.
4. Fitzky, B. U., M. Witsch-Baumgartner, M. Erdel, J. N. Lee, Y. K. Paik, H. Glossmann, G. Utermann, and F. F. Moebius. 1998. Mutations in the Δ7-sterol reductase gene in patients with the Smith-Lemli-Opitz syndrome. Proc. Natl. Acad. Sci. USA 95:8181-8186.
5. Fryberg, M., A. C. Oehlschlager, and M. Unrau. 1973. Biosynthesis of ergosterol in yeast: evidence for multiple pathways. J. Am. Chem. Sci. 95:5747-5757.
6. Gaylor, J. L. 1981. Formation of sterols in animals, p. 481-543. In J. W. Porter and S. L. Springer (ed.), Biosynthesis of isoprenoid compounds. Wiley, New York, NY.
7. Hartmann, M. A., and P. Benveniste. 1987. Plant membrane sterols: isolation, identification, and biosynthesis. Methods Enzymol. 148:632-650.
8. Hayashi, Y., and M. Nakajima. May 2004. Manufacturing process for fungi improved by metabolic engineering and sterols produced by them. Japan patent 2004-141125.
9. Lecain, E., X. Chenivesse, R. Spagnoli, and D. Pompon. 1996. Cloning by metabolic interference in yeast and enzymatic characterization of Arabidopsis thaliana sterol Δ7-reductase. J. Biol. Chem. 271:10866-10873.
10. Lee, J., and Y. K. Paik. 1997. Cholesterol biosynthesis from lanosterol: development of a novel assay method, characterization, and solubilization of rat hepatic microsomal sterol Δ7-reductase. J. Biochem. Mol. Biol. 30:370-377.
11. Moebius, F. F., B. U. Fitzky, J. N. Lee, Y. K. Paik, and H. Glossmann. 1998. Molecular cloning and expression of the human Δ7-sterol reductase. Proc. Natl. Acad. Sci. USA 95:1899-1902.
12. Nes, W. D., and S. D. Nichols. 2006. Phytosterol biosynthesis pathway in Mortierella alpina. Phytochemistry 67:1716-1721.
13. Osumi, T., S. Taketani, H. Katsuki, T. Kuhara, and I. Matsumoto. 1978. Ergosterol biosynthesis in yeast. Pathways in the late stages and their variation under various conditions. J. Biochem. 83:681-691.
14. Popjak, G., A. Meenan, E. J. Parish, and W. D. Nes. 1989. Inhibition of cholesterol synthesis and cell growth by 24(R,S),25- iminolanosterol and triparanol in cultured rat hepatoma cells. J. Biol. Chem. 264:6230-6238.
15. Reinhart, M. P., J. T. Billheimer, J. R. Faust, and J. L. Gaylor. 1987. Subcellular localization of the enzymes of cholesterol biosynthesis and metabolism in rat liver. J. Biol. Chem. 262:9649-9655.
16. Sakuradani, E., T. Abe, K. Iguchi, and S. Shimizu. 2005. A novel fungal ω3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 66:648-654.
17. 9-Fatty acid desaturase from arachidonic acid-producing fungus: unique gene sequence and its heterologous expression in a fungus, Aspergillus. Eur. J. Biochem. 260:208-216.
18. Schroepfer, G. J., Jr. 1982. Sterol biosynthesis. Annu. Rev. Biochem. 51: 555-585.
19. Shefer, S., G. Salen, A. Honda, A. BaKa, S. Hauser, G. S. Tint, M. Honda, T. Chen, M. F. Holick, and L. B. Nguyen. 1997. Rapid identification of Smith-Lemli-Opitz syndrome homozygotes and heterozygotes (carriers) by measurement of deficient 7-dehydrocholesterol-Δ7-reductase activity in fibroblasts. Metabolism 46:844-850.
20. Shimizu, S., H. Kawashima, M. Wada, and H. Yamada. 1992. Occurrence of a novel sterol, 24,25-methylenecholest-5-en-3β-ol, in Mortierella alpina 1S-4. Lipids 27:481-483.
21. Smith, D. W., L. Lemli, and J. M. Opitz. 1964. A newly recognized syndrome of multiple congenital anomalies. J. Pediatr. 64:210-217.
22. Szczebara, F. M., C. Chandelier, C. Villeret, A. Masurel, S. Bourot, C. Duport, S. Blanchard, A. Groisillier, E. Testet, P. Costaglioli, G. Cauet, E. Degryse, D. Balbuena, J. Winter, T. Achstetter, R. Spagnoli, D. Pompon, and B. Dumas. 2003. Total biosynthesis of hydrocortisone from a simple carbon source in yeast. Nat. Biotechnol. 21:143-149.
23. Takeno, S., E. Sakuradani, S. Murata, M. Inohara-Ochiai, H. Kawashima, T. Ashikari, and S. Shimizu. 2004. Cloning and sequencing of the ura3 and ura5 genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4. Biosci. Biotechnol. Biochem. 68:277-285.
24. Takeno, S., E. Sakuradani, S. Murata, M. Inohara-Ochiai, H. Kawashima, T. Ashikari, and S. Shimizu. 2004. Establishment of an overall transformation system for an oil-producing filamentous fungus, Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 65:419-425.
25. Takeno, S., E. Sakuradani, S. Murata, M. Inohara-Ochiai, H. Kawashima, T. Ashikari, and S. Shimizu. 2005. Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase. Lipids 40:25-30.
26. Takeno, S., E. Sakuradani, A. Tomi, M. Inohara-Ochiai, H. Kawashima, T. Ashikari, and S. Shimizu. 2005. Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S-4, through RNA interference with Δ12-desaturase gene expression. Appl. Environ. Microbiol. 71:5124-5128.
27. Tint, G. S., M. Irons, E. R. Elias, A. K. Batta, R. Frieden, T. S. Chen, and G. Salen. 1994. Defective cholesterol biosynthesis associated with the Smith-Lemli-Opitz syndrome. N. Engl. J. Med. 13:107-113.
28. Waterham, H. R., F. A. Wijburg, R. C. Hennekam, P. Vreken, B. T. Poll-The, L. Dorland, M. Duran, P. E. Jira, J. A. Smeitink, R. A. Wevers, and R. J. Wanders. 1998. Smith-Lemli-Opitz syndrome is caused by mutations in the 7-dehydrocholesterol reductase gene. Am. J. Hum. Genet. 63:329-338.
29. Yamada, H., S. Shimizu, and Y. Shinmen. 1987. Production of arachidonic acid by Mortierella elongata 1S-5. Agric. Biol. Chem. 51:785-790.
30. Yu, H., and S. B. Patel. 2005. Recent insights into the Smith-Lemli-Opitz syndrome. Clin. Genet. 68:383-391.