New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database

Journal of Mass Spectrometry

Volumn 42, Issue 3, 2007, Pages 288-292

  Basilico, F ^a   Di Silvestre, D ^a   Sedini, S ^a   Petretto, A ^b   Levreri, I ^b   Melioli, G ^b   Farina, C ^c   Mori, F ^c   Mauri, P L ^a  

^a CNR   (Italy)

^b Department of Experimental and Laboratory Medicine ^*  (Italy)

^c Kedrion SpA   (Italy)

Author keywords

Hemoglobin variants; LC MS MS; SEQUEST; Tryptic peptide database

Indexed keywords

ALGORITHMS; DATABASE SYSTEMS; ELECTROPHORESIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES;

HEMOGLOBIN VARIANTS; SEQUEST ALGORITHM; TRYPTIC PEPTIDE DATABASE;

HEMOGLOBIN;

HEMOGLOBIN VARIANT; PEPTIDE;

ALGORITHM; ARTICLE; CORRELATION ANALYSIS; DATA BASE; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN ANALYSIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; DATABASES, PROTEIN; HEMOGLOBINS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PEPTIDES; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; VARIATION (GENETICS);

EID: 33947268640     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1160     Document Type: Article

References (21)

1. http://www.expasy.org [2006].
2. http://www.ncbi.nlm.nhi.gov [2006].
3. http://globin.bx.psu.edu/hbvar/menu.html [2006].
4. Wild BJ, Bain BJ. Detection and quantitation of normal and variant haemoglobins: an analytical review. Ann. Clin. Biochem. 2004; 41: 355.
5. Serjeant GR, Serjeant BE. In Sickle Cell Disease, 3rd edn. Oxford University Press: Oxford, 2001.
6. Weatherall DJ, Clegg JB. In The Thalassemia Syndromes, 4th edn. Blackwell Scientific: Oxford. 2001.
7. Huisman T, Carver M, Efremov G. In Human Haemoglobin Variants, 2nd edn. Sickle Cell Anemia foundation: Augusta, 1998.
8. Righetti PG, Gianazza E, Bianchi-Bosisio A, Cossu G. In Methods in Hematology, Huisman THJ (ed). Churchill Livingstone: Edinburgh, 1986.
9. OU CN, Rognerud CL. Rapid analysis of hemoglobin variants by cation-exchange HPLC. Clin. Chem. 1993; 39: 820.
10. Higgins TN, Ridley B, Clarket G. Novel method for screening for the presence of hemoglobin S in blood. Transfus. Med. 2005; 15: 493.
11. Huisman THJ. High performance liquid chromatographic analysis of human hemoglobins and their polypeptide chains: its use in the identification of variants. Anal. Chim. Acta 1997; 352: 187.
12. Rai DK, Griffiths WJ, Landin B, Wild BJ, Alvelius G, Green BN. Accurate mass measurement by electrospray ionization quadrupole mass spectrometry: detection of variants differing by <6 Da from normal in human hemoglobin heterozygotes. Anal. Chem. 2003; 75: 1978.
13. Caruso D, Crestani M, Mitro N, Da Riva L, Mozzi R, Sarpau S, Merlotti C, Franzini C. High pressure liquid chromatography and electrospray ionization mass spectrometry are advantageously integrated into a two-level approach to detection and identification of haemoglobin variants. Clin. Lab. Haematol. 2005; 27: 111.
14. Yang L, Lee CS, Hofstadler S, Pasa-Tolic L, Smith RD. Capillary isoelectric focusing-electrospray ionization fourier transform ion cyclotron resonance mass spectrometry for protein characterization. Anal. Chem. 1998; 70: 3235.
15. Rai DK, Green BN, Landin B, Alvelius G, Griffiths WJ. Accurate mass measurement and tandem mass spectrometry of intact globin chains identify the low portion variant hemoglobin Lepore-Boston-Washington from the blood of a heterozygote. J. Mass Spectrom. 2004; 39: 289.
16. Gatlin CL, Eng JK, Cross ST, Detter JC, Yates JR III. Automated identifications of amino acid sequence variations in proteins by HPLC/Microspray tandem mass spectrometry. Anal. Chem. 2000; 72: 757.
17. Yates JR III, Eng J, McCormac A, Schieltz D. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database. Anal Chem. 1995; 67: 1426.
18. Hubbard M, Wilson JB, Wrightstone RN, Efremov GD, Huisman TH. Hb Cadmen and Hb Hope found during routine testing. Acta Haematol. 1975; 54: 53.
19. Garel MC, Hassan W, Coquelet MT, Goossens M, Rosa J, Arous N. Hemoglobin J Cairo: beta 65 (E9) Lys leads to Gln, a new hemoglobin variant discovered in an Egyptian family. Biochim. Biophys. Acta 1976; 420: 97.
20. Makarov A, Denisov E, Kholomeev A, Balschum W, Lange O, Strupat K, Horning S. Performance evaluation of a hybrid linear Ion trap/Orbitrap Mass spectrometry. Anal. Chem. 2006; 78: 2113.
21. Shackleton CH, Witkowska HE. Characterizing abnormal hemoglobin by MS. Anal. Chem. 1996; 68: 29A.