Functional definition of the tobacco protoporphyrinogen IX oxidase substrate-binding site

Biochemical Journal

Volumn 402, Issue 3, 2007, Pages 575-580

  Heinemann, Ilka U ^a   Diekmann, Nina ^a   Masoumi, Ava ^a   Koch, Michael ^b   Messerschmidt, Albrecht ^c   Jahn, Martina ^a   Jahn, Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Enzyme kinetics; Haem biosynthesis; Protoporphyrin; Protoporphyrinogen IX oxidase; Stopped flow fluorescence; Variegate porphyria

Indexed keywords

AMINO ACIDS; BINDING ENERGY; BIOSYNTHESIS; CHLOROPHYLL; PORPHYRINS;

HAEM BIOSYNTHESIS; PROTOPORPHYRIN; PROTOPORPHYRINOGEN IX OXIDASE; STOPPED-FLOW FLUORESCENCE; VARIEGATE PORPHYRIA;

ENZYME KINETICS;

PROTOPORPHYRINOGEN OXIDASE;

ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME KINETICS; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; TOBACCO;

AMINO ACIDS; BINDING SITES; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN STRUCTURE, TERTIARY; PROTOPORPHYRINOGEN OXIDASE; PROTOPORPHYRINS; SUBSTRATE SPECIFICITY; TOBACCO;

NICOTIANA TABACUM;

EID: 33947230298     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20061321     Document Type: Article

References (32)

1. Brenner, D. A. and Bloomer, J. R. (1980) The enzymatic defect in variegate porphyria. Studies with human cultured skin fibroblasts. N. Engl. J. Med. 302, 765-769
2. Poulson, R. and Polglase, W. J. (1975) The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX. Protoporphyrinogen oxidase activity in mitochondrial extracts of Saccharomyces cerevisiae. J. Biol. Chem. 250, 1269-1274
3. Poulson, R. (1976) The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX in mammalian mitochondria. J. Biol. Chem. 251, 3730-3733
4. Dailey, H. A. and Karr, S. W. (1987) Purification and characterization of murine protoporphyrinogen oxidase. Biochemistry 26, 2697-2701
5. Hansson, M. and Hederstedt, L. (1992) Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes. J. Bacteriol. 174, 8081-8093
6. Siepker, L. J., Ford, M., de Kock, R. and Kramer, S. (1987) Purification of bovine protoporphyrinogen oxidase: immunological cross-reactivity and structural relationship to ferrochelatase. Biochim. Biophys. Acta 913, 349-358
7. Dailey, T. A. and Dailey, H. A. (1996) Human protoporphyrinogen oxidase: expression, purification, and characterization of the cloned enzyme. Protein Sci. 5, 98-105
8. Dailey, T. A. and Dailey, H. A. (1997) Expression, purification, and characteristics of mammalian protoporphyrinogen oxidase. Methods Enzymol. 281, 340-349
9. Maneli, M. H., Corrigall, A. V., Klump, H. H., Davids, L. M., Kirsch, R. E. and Meissner, P. N. (2003) Kinetic and physical characterisation of recombinant wild-type and mutant human protoporphyrinogen oxidases. Biochim. Biophys. Acta 1650, 10-21
10. Deybach, J. C., da Silva, V., Grandchamp, B. and Nordmann, Y. (1985) The mitochondrial location of protoporphyrinogen oxidase. Eur. J. Biochem. 149, 431-435
11. Jacobs, J. M. and Jacobs, N. J. (1984) Protoporphyrinogen oxidation, an enzymatic step in heme and chlorophyll synthesis: partial characterization of the reaction in plant organelles and comparison with mammalian and bacterial systems. Arch. Biochem. Biophys. 229, 312-319
12. Dailey, H. A. (2002) Terminal steps of haem biosynthesis. Biochem. Soc. Trans. 30, 590-595
13. Kappas, A. S. S., Galbraith, R. A. and Nordmann, Y. (1995) The Metabolic and Molecular Basis of Inherited Diseases, McGraw-Hill, New York
14. Mustajoki, P. (1980) Variegate porphyria. Twelve years' experience in Finland. Q. J. Med. 49, 191-203
15. Dean, G. (1971) Screening tests for porphyria. Lancet 1, 86-87
16. Meissner, P. N., Dailey, T. A., Hift, R. J., Ziman, M., Corrigall, A. V., Roberts, A. G., Meissner, D. M., Kirsch, R. E. and Dailey, H. A. (1996) A R59W mutation in human protoporphyrinogen oxidase results in decreased enzyme activity and is prevalent in South Africans with variegate porphyria. Nat. Genet. 13, 95-97
17. Warnich, L., Kotze, M. J., Groenewald, I. M., Groenewald, J. Z., van Brakel, M. G., van Heerden, C. J., de Villiers, J. N., van de Ven, W. J., Schoenmakers, E. F., Taketani, S. et al. (1996) Identification of three mutations and associated haplotypes in the protoporphyrinogen oxidase gene in South African families with variegate porphyria. Hum. Mol. Genet. 5, 981-984
18. Roberts, A. G., Puy, H., Dailey, T. A., Morgan, R. R., Whatley, S. D., Dailey, H. A., Martasek, P., Nordmann, Y., Deybach, J. C. and Elder, G. H. (1998) Molecular characterization of homozygous variegate porphyria. Hum. Mol. Genet. 7, 1921-1925
19. von und zu Fraunberg, M., Timonen, K., Mustajoki, P. and Kauppinen, R. (2002) Clinical and biochemical characteristics and genotype-phenotype correlation in Finnish variegate porphyria patients. Eur. J. Hum. Genet. 10, 649-657
20. Lermontova, I., Kruse, E., Mock, H. P. and Grimm, B. (1997) Cloning and characterization of a plastidal and a mitochondrial isoform of tobacco protoporphyrinogen IX oxidase. Proc. Natl. Acad. Sci. U.S.A. 94, 8895-8900
21. Beale, S. I. (1999) Enzymes of chlorophyll biosynthesis. Photosynth. Res. 60, 43-73
22. Koch, M., Breithaupt, C., Kiefersauer, R., Freigang, J., Huber, R. and Messerschmidt, A. (2004) Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis. EMBO J. 23, 1720-1728
23. Phillips, J. D., Whitby, F. G., Kushner, J. P. and Hill, C. P. (2003) Structural basis for tetrapyrrole coordination by uroporphyrinogen decarboxylase. EMBO J. 22, 6225-6233
24. Breckau, D., Mahlitz, E., Sauerwald, A., Layer, G. and Jahn, D. (2003) Oxygen-dependent coproporphyrinogen III oxidase (HemF) from Escherichia coli is stimulated by manganese. J. Biol. Chem. 278, 46625-46631
25. Layer, G., Grage, K., Teschner, T., Schunemann, V., Breckau, D., Masoumi, A., Jahn, M., Heathcote, P., Trautwein, A. X. and Jahn, D. (2005) Radical S-adenosylmethionine enzyme coproporphyrinogen III oxidase HemN: functional features of the [4Fe-4S] cluster and the two bound S-adenosyl-L-methionines. J. Biol. Chem. 280, 29038-29046
26. Randau, L., Schauer, S., Ambrogelly, A., Salazar, J. C., Moser, J., Sekine, S., Yokoyama, S., Soll, D. and Jahn, D. (2004) tRNA recognition by glutamyl-tRNA reductase. J. Biol. Chem. 279, 34931-34937
27. Ohno, Y., Buescher, E. S., Roberts, R., Metcalf, J. A. and Gallin, J. I. (1986) Reevaluation of cytochrome b and flavin adenine dinucleotide in neutrophils from patients with chronic granulomatous disease and description of a family with probable autosomal recessive inheritance of cytochrome b deficiency. Blood 67, 1132-1138
28. Labbe, P., Camadro, J. M. and Chambon, H. (1985) Fluorometric assays for coproporphyrinogen oxidase and protoporphyrinogen oxidase. Anal. Biochem. 149, 248-260
29. Layer, G., Moser, J., Heinz, D. W., Jahn, D. and Schubert, W. D. (2003) Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of radical SAM enzymes. EMBO J. 22, 6214-6224
30. Corrigall, A. V., Siziba, K. B., Maneli, M. H., Shephard, E. G., Ziman, M., Dailey, T. A., Dailey, H. A., Kirsch, R. E. and Meissner, P. N. (1998) Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis. Arch. Biochem. Biophys. 358, 251-256
31. Camadro, J. M., Thome, F., Brouillet, N. and Labbe, P. (1994) Purification and properties of protoporphyrinogen oxidase from the yeast Saccharomyces cerevisiae. Mitochondrial location and evidence for a precursor form of the protein. J. Biol. Chem. 269, 32085-32091
32. Wang, K. F., Dailey, T. A. and Dailey, H. A. (2001) Expression and characterization of the terminal heme synthetic enzymes from the hyperthermophile Aquifex aeolicus. FEMS Microbiol. Lett. 202, 115-119