In situ extraction of intracellular l-asparaginase using thermoseparating aqueous two-phase systems

Journal of Chromatography A

Volumn 1147, Issue 1, 2007, Pages 127-134

  Zhu, Jian Hang ^a,b   Yan, Xi Luan ^b   Chen, Hong Jun ^b   Wang, Zhi Hui ^b  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b NANCHANG UNIVERSITY   (China)

Author keywords

Aqueous two phase extraction system; Cell disruption; In situ extraction; Intracellular protein; Process integration

Indexed keywords

CELLS; CHROMATOGRAPHY; ESCHERICHIA COLI; PHASE SEPARATION; PURIFICATION; THERMOANALYSIS;

AQUEOUS TWO PHASE EXTRACTION SYSTEMS; CELL DISRUPTION; IN SITU EXTRACTION; INTRACELLULAR PROTEINS; PROCESS INTEGRATION;

ENZYMES;

ASPARAGINASE; CELL ENZYME;

ACIDITY; ARTICLE; CELL DISRUPTION; CENTRIFUGATION; ENZYMATIC DEGRADATION; ENZYME PURIFICATION; EXTRACTION; PRIORITY JOURNAL;

ASPARAGINASE; BIOREACTORS; BIOTECHNOLOGY; CELL EXTRACTS; CELL FRACTIONATION; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; POLYETHYLENE GLYCOLS; PROTEINS; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 33947228400     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2007.02.035     Document Type: Article

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