Lateral diffusion of TGF-β type I receptor studied by single-molecule imaging

Biochemical and Biophysical Research Communications

Volumn 356, Issue 1, 2007, Pages 67-71

  Ma, Xinyong ^a   Wang, Qiang ^b   Jiang, Yaxin ^a   Xiao, Zeyu ^a   Fang, Xiaohong ^a   Chen, Ye Guang ^b  

^a INSTITUTE OF CHEMISTRY   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Lipid raft; Mobility; Single molecule fluorescence imaging; TGF receptor; TGF 1

Indexed keywords

CHOLESTEROL; GREEN FLUORESCENT PROTEIN; METHYL BETA CYCLODEXTRIN; NYSTATIN; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 1; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 2; TRANSFORMING GROWTH FACTOR BETA1;

ARTICLE; CELL MEMBRANE; DIFFUSION; FLUORESCENCE; HUMAN; HUMAN CELL; LIPID RAFT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ACTIVIN RECEPTORS, TYPE I; CELL MEMBRANE; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IONOPHORES; MEMBRANE MICRODOMAINS; MICROSCOPY, FLUORESCENCE; NYSTATIN; PROTEIN TRANSPORT; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 33947146085     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.02.080     Document Type: Article

References (18)

1. Massague J., and Chen Y.-G. Controlling TGF-β signaling. Genes Dev. 14 (2000) 627-644
2. Wrana J.L., Attisano L., Carcamo J., Zentella A., Doody J., Laiho M., Wang X., and Massagure J. TGFβ signals through a heteromeric protein kinase receptor complex. Cell 71 (1992) 1003-1014
3. Yamashita H., ten Dijke P., Franzen P., Miyazono K., and Heldin C. Formation of hetero-oligomeric complexes of type I and type II receptors for transforming growth factor-β. J. Biol. Chem. 269 (1994) 20172-20178
4. Zuniga J.E., Groppe J.C., Cui Y., Hinck C.S., Contreras-Shannon V., Pakhomova O.N., Yang J., Tang Y., Mendoza V., Lopez-Casillas F., Sun L.Z., and Hinck A.P. Assembly of TβRI: TβRII: TGFβ ternary complex in vitro with receptor extracellular domains is cooperative and isoform-dependent. J. Mol. Biol. 354 (2005) 1052-1054
5. Sako Y., and Uyemura T. Total internal reflection fluorescence microscopy for single-molecule imaging in living cells. Cell Struct. Funct. 27 (2002) 357-365
6. Sako Y., Minoguchi S., and Yanagida T. Single-molecule imaging of EGFR signalling on the surface of living cells. Nat. Cell Biol. 2 (2000) 168-172
7. Iino R., Koyama I., and Kusumi A. Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface. Biophys. J. 80 (2001) 2667-2677
8. Murakoshi H., Iino R., Kobayashi T., Fujiwara T., Ohshima C., Yoshimura A., and Kusumi A. Single-molecule imaging analysis of Ras activation in living cells. Proc. Natl. Acad. Sci. 101 (2004) 7317-7322
9. Tardin C., Cognet L., Bats C., Lounis B., and Choquet D. Direct imaging of lateral movements of AMPA receptors inside synapses. EMBO J. 22 (2003) 4656-4665
10. Orr G., Hu D., Ozcxelik S., Opresko L.K., Wiley H.S., and Colson S.D. Cholesterol dictates the freedom of EGF receptors and HER2 in the plane of the membrane. Biophys. J. 89 (2005) 1362-1373
11. Yao G., Fang X., Yokota H., Yanagida T., and Tan W. Monitoring molecular beacon DNA probe hybridization at the single-molecule level. Chem. Eur. J. 9 (2003) 5686-5692
12. Kusumi A., Sako Y., and Yamamoto M. Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells. Biophys. J. 65 (1993) 2021-2040
13. Lu Z.X., Murray J.T., Luo W.J., Li H.L., Wu X.P., Xu H.X., Backer J.M., and Chen Y.-G. Transforming growth factor β activates smad2 in the absence of receptor endocytosis. J. Biol. Chem. 277 (2002) 29363-29368
14. Gilboa L., Wells R.G., Lodish H.F., and Henis Y.I. Oligomeric structure of type I and type II transforming growth factor-β receptors: homodimers form in the ER and persist at the plasma membrane. J. Cell Biol. 140 (1998) 767-777
15. Roy C.L., and Wrana J.L. Clathrin- and non-clathrin mediated endocytic regulation of cell signalling. Nat. Rev. Mol. Cell. Biol. 6 (2005) 112-126
16. Razani B., Zhang X.L., Bitzer M., von Gersdorff G., Böttinger E.P., and Lisanti M.P. Caveolin-1 regulates transforming growth factor (TGF)-β/SMAD signaling through an interaction with the TGF-β type I receptor. J. Biol. Chem. 276 (2001) 6727-6738
17. Di Guglielmo G.M., Roy C.L., Goodfellow A.F., and Wrana J.L. Distinct endocytic pathways regulate TGF-β receptor signalling and turnover. Nat. Cell Biol. 5 (2003) 410-421
18. Xiao L.Z., Nicholas T., Takafumi I., and Aled P. Interleukin-6 regulation of transforming growth factor (TGF)-β receptor compartmentalization and turnover enhances TGF-β1 signaling. J. Biol. Chem. 280 (2005) 12239-12245