Reduction of O-GlcNAc protein modification does not prevent insulin resistance in 3T3-L1 adipocytes

American Journal of Physiology - Endocrinology and Metabolism

Volumn 292, Issue 3, 2007, Pages

  Robinson, Katherine A ^a   Ball, Lauren E ^a   Buse, Maria G ^a,b  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b CSB 823 ^*  (United States)

Author keywords

Akt activation; Glucose transport; O linked N acetylglucosamine

Indexed keywords

ACETYLGLUCOSAMINIDASE; GLUCOSE; INSULIN; N ACETYLGLUCOSAMINE; N ACETYLGLUCOSAMINYLTRANSFERASE; PROTEIN KINASE B; SERINE; SMALL INTERFERING RNA; THREONINE;

ACETYLATION; ADIPOCYTE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; GLUCOSE TRANSPORT; INSULIN RESISTANCE; LOW DRUG DOSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MODIFICATION; WESTERN BLOTTING;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; GLUCOSE; GLYCOSYLATION; INSULIN; INSULIN RESISTANCE; MICE; N-ACETYLGLUCOSAMINYLTRANSFERASES; RNA, SMALL INTERFERING; TRANSDUCTION, GENETIC;

EID: 33947109881     PISSN: 01931849     EISSN: 15221555     Source Type: Journal    
DOI: 10.1152/ajpendo.00569.2006     Document Type: Article

References (34)

1. Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. Diabetes 54: 1615-1625, 2005.
2. Buse MG. Hexosamines, insulin resistance, and the complications of diabetes: current status. Am J Physiol Endocrinol Metab 290: E1-E8, 2006.
3. Comer FI, Hart GW. Reciprocity between O-GlcNAc and O-phosphate on the carboxyl terminal domain of RNA polymerase II. Biochemistry 40: 7845-7852, 2001.
4. Comer FI, Vosseller K, Wells L, Accavitti MA, Hart GW. Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine. Anal Biochem 293: 169-177, 2001.
5. Cooksey RC, Hebert LF, Zhu JH, Wofford P, Garvey WT, McClain DA. Mechanism of hexosamine-induced insulin resistance in transgenic mice overexpressing glutamine:fructose-6-phosphate amidotransferase: decreased glucose transporter GLUT4 translocation and reversal by treatment with thiazolidinedione. Endocrinology 140: 1151-1157, 1999.
6. Deshayes S, Gerbal-Chaloin S, Morris MC, Aldrian-Herrada G, Charnet P, Divita G, Heitz F. On the mechanism of non-endosomial peptide-mediated cellular delivery of nucleic acids. Biochim Biophys Acta 1667: 141-147, 2004.
7. Dignam JD, Lebovitz RM, Roeder RG. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11: 1475-1489, 1983.
8. Dong DL, Hart GW. Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol. J Biol Chem 269: 19321-19330, 1994.
9. Gao Y, Wells L, Comer FI, Parker GJ, Hart GW. Dynamic Oglycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain. J Biol Chem 276: 9838-9845, 2001.
10. Garvey WT, Olefsky JM, Matthaei S, Marshall S. Glucose and insulin co-regulate the glucose transport system in primary cultured adipocytes. A new mechanism of insulin resistance. J Biol Chem 262: 189-197, 1987.
11. Goldberg HJ, Whiteside CI, Hart GW, Fantus IG. Posttranslational, reversible O-glycosylation is stimulated by high glucose and mediates plasminogen activator inhibitor-1 gene expression and Sp1 transcriptional activity in glomerular mesangial cells. Endocrinology 147: 222-231, 2006.
12. Hazel M, Cooksey RC, Jones D, Parker G, Neidigh JL, Witherbee B, Gulve EA, McClain DA. Activation of the hexosamine signaling pathway in adipose tissue results in decreased serum adiponectin and skeletal muscle insulin resistance. Endocrinology 145: 2118-2128, 2004.
13. He TC, Kinzler KW, Vogelstein B. A simplified system for rapid generation of recombinant adenoviruses. A practical guide to using the AdEasy system [Online]. Howard Hughes Medical Institute and Molecular Genetics Laboratory, Johns Hopkins Oncology Center. http://www.coloncancer.org/adeasy/ protocol.htm [2006].
14. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci USA 95: 2509-2514, 1998.
15. Hebert LF, Daniels MC, Zhou J, Crook ED, Turner RL, Simmons ST, Neidigh JL, Zhu JS, Baron AD, McClain DA. Overexpression of glutamine:fructose-6-phosphate amidotransferase in transgenic mice leads to insulin resistance. J Clin Invest 98: 930-936, 1996.
16. Hu Y, Belke D, Suarez J, Swanson E, Clark R, Hoshijima M, Dillmann WH. Adenovirus-mediated overexpression of O-GlcNAcase improves contractile function in the diabetic heart. Circ Res 96: 1006-1013, 2005.
17. Kreppel LK, Blomberg MA, Hart GW. Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats. J Biol Chem 272: 9308-9315, 1997.
18. Lubas WA, Frank DW, Krause M, Hanover JA. O-Linked GlcNAc transferase is a conserved nucleocytoplasmic protein containing tetratricopeptide repeats. J Biol Chem 272: 9316-9324, 1997.
19. Marshall S, Bacote V, Traxinger RR. Discovery of a metabolic pathway mediating glucose-induced desensitization of the glucose transport system. Role of hexosamine biosynthesis in the induction of insulin resistance. J Biol Chem 266: 4706-4712, 1991.
20. McClain DA. Hexosamines as mediators of nutrient sensing and regulation in diabetes. J Diabetes Complications 16: 72-80, 2002.
21. McClain DA, Lubas WA, Cooksey RC, Hazel M, Parker GJ, Love DC, Hanover JA. Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia. Proc Natl Acad Sci USA 99: 10695-10699, 2002.
22. Mitra P, Zheng X, Czech MP. RNAi-based analysis of CAP, Cbl, and CrkII function in the regulation of GLUT4 by insulin. J Biol Chem 279: 37431-37435, 2004.
23. Nelson BA, Robinson KA, Buse MG. High glucose and glucosamine induce insulin resistance via different mechanisms in 3T3-L1 adipocytes. Diabetes 49: 981-991, 2000.
24. Nelson BA, Robinson KA, Buse MG. Insulin acutely regulates Munc18-c subcellular trafficking: altered response in insulin-resistant 3T3-L1 adipocytes. J Biol Chem 277: 3809-3812, 2002.
25. Nelson BA, Robinson KA, Buse MG. Defective Akt activation is associated with glucose- but not glucosamine-induced insulin resistance. Am J Physiol Endocrinol Metab 282: E497-E506, 2002.
26. Obici S. Hexosamine pathway contribution to insulin resistance. New insights from muscle specific GFAT transgenic. 65th Annual Meeting of the American Diabetes Association, San Diego, CA, 2005.
27. Rossetti L, Giaccari A, DeFronzo RA. Glucose toxicity. Diabetes Care 13: 610-630, 1990.
28. Rossetti L. Perspective: Hexosamines and nutrient sensing. Endocrinology 141: 1922-1925, 2000.
29. Toleman C, Paterson AJ, Whisenhunt TR, Kudlow JE. Characterization of the histone acetyltransferase (HAT) domain of a bifunctional protein with activable O-GlcNAcase and HAT activities. J Biol Chem 279: 53665-53673, 2004.
30. Vosseller K, Wells L, Lane MD, Hart GW. Elevated nucleocytoplasmic glycosylation by O-GlcNAc results in insulin resistance associated with defects in Akt activation in 3T3-L1 adipocytes. Proc Natl Acad Sci USA 99: 5313-5318, 2002.
31. Wells L, Vosseller K, Hart GW. Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc. Science 291: 2376-2378, 2001.
32. Wells L, Gao Y, Mahoney JA, Vosseller K, Chen C, Rosen A, Hart GW. Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase. J Biol Chem 277: 1755-1761, 2002.
33. Whisenhunt TR, Yang X, Bowe DB, Paterson AJ, Van Tine BA, Kudlow JE. Disrupting the enzyme complex regulating O-GlcNAcylation blocks signaling and development. Glycobiology 16: 551-563, 2006.
34. Yki-Jarvinen H, Helve E, Koivisto VA. Hyperglycemia decreases glucose uptake in type I diabetes. Diabetes 36: 892-896, 1987.