A method for single-cell sorting and expansion of genetically modified human embryonic stem cells

Stem Cells and Development

Volumn 16, Issue 1, 2007, Pages 109-117

  Nicholas, Cory R ^a   Gaur, Meenakshi ^a   Wang, Shaohui ^a   Reijo Pera, Renee A ^a   Leavitt, Andrew D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL DIVISION; CULTURE MEDIUM; DIPLOIDY; DNA MODIFICATION; EMBRYO; EMBRYONIC STEM CELL; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENE FUNCTION; HUMAN; HUMAN CELL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; TISSUE SPECIFICITY; TRANSGENE;

CELL DIFFERENTIATION; CELL SEPARATION; CLONE CELLS; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; PLURIPOTENT STEM CELLS; TRANSDUCTION, GENETIC; TRANSGENES;

LENTIVIRUS;

EID: 33847689843     PISSN: 15473287     EISSN: None     Source Type: Journal    
DOI: 10.1089/scd.2006.0059     Document Type: Article

References (32)

1. Keller G. (2005). Embryonic stem cell differentiation: emergence of a new era in biology and medicine. Genes Dev 19:1129-1155.
2. Ben-Dor I, P Itsykson, D Goldenberg, E Galun and BE Reubinoff. (2006). Lentiviral vectors harboring a dual-gene system allow high and homogeneous transgene expression in selected polyclonal human embryonic stem cells. Mol Ther 14:255-267.
3. Heidersbach A, A Gaspar-Maia, MT McManus and M Ramalho-Santos. (2006). RNA interference in embryonic stem cells and the prospects for future therapies. Gene Ther 13:478-486.
4. Menendez P, L Wang and M Bhatia. (2005). Genetic manipulation of human embryonic stem cells: a system to study early human development and potential therapeutic applications. Curr Gene Ther 5:375-385.
5. Ma Y, A Ramezani, R Lewis, R Hawley and J Thomson. (2003). High-level sustained transgene expression in human embryonic stem cells using lend viral vectors. Stem Cells 21:111-117.
6. Eiges R, M Schuldiner, M Drukker, O Yanuka, J Itskovitz-Eldor and N Benvenisty. (2001). Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells. Curr Biol 11:514-518.
7. Vallier L, PJ Rugg-Gunn, IA Bouhon, FK Andersson, AJ Sadler and RA Pedersen. (2004). Enhancing and diminishing gene function in human embryonic stem cells. Stem Cells 22:2-11.
8. Ren CP, M Zhao, WJ Shan, XY Yang, ZH Yin, XJ Jiang, HB Zhang and KT Yao. (2005). Establishment of human embryonic stem cell line stably expressing Epstein-Barr virus-encoded nuclear antigen 1. Acta Biochim Biophys Sin (Shanghai) 37:68-73.
9. Liu YP, OV Dovzhenko, MA Garthwaite, SV Dambaeva, M Durning, LM Pollastrini and TG Golos. (2004). Maintenance of pluripotency in human embryonic stem cells stably over-expressing enhanced green fluorescent protein. Stem Cells Dev 13:636-645.
10. Zwaka TP and JA Thomson. (2003). Homologous recombination in human embryonic stem cells. Nature Biotechnol 21:319-321.
11. Lakshmipathy U, B Pelacho, K Sudo, JL Linehan, E Coucouvanis, DS Kaufman and CM Verfaillie. (2004). Efficient transfection of embryonic and adult stem cells. Stem Cells 22:531-543.
12. Siemen H, M Nix, E Endl, P Koch, J Itskovitz-Eldor and O Brustle. (2005). Nucleofection of human embryonic stem cells. Stem Cells Dev 14:378-383.
13. Pfeifer A, M Ikawa, Y Dayn and IM Verma. (2002). Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA 99:2140-2145.
14. Gropp M, P Itsykson, O Singer, T Ben-Hur, E Reinhartz, E Galun and BE Reubinoff. (2003). Stable genetic modification of human embryonic stem cells by lentiviral vectors. Mol Ther 7:281-287.
15. Xiong C, DQ Tang, CQ Xie, L Zhang, KF Xu, WE Thompson, W Chou, GH Gibbons, LJ Chang, LJ Yang and YE Chen. (2005). Genetic engineering of human embryonic stem cells with lentiviral vectors. Stem Cells Dev 14:367-377.
16. Suter DM, L Cartier, E Bettiol, D Tirefort, ME Jaconi, M Dubois-Dauphin and KH Krause. (2006). Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors. Stem Cells 24:615-623.
17. Zaehres H, MW Lensch, L Daheron, SA Stewart, J Itskovitz-Eldor and GQ Daley. (2005). High-efficiency RNA interference in human embryonic stem cells. Stem Cells 23:299-305.
18. Clark AT, MS Bodnar, M Fox, RT Rodriquez, MJ Abeyta, MT Firpo and RA Pera. (2004). Spontaneous differentiation of germ cells from human embryonic stem cells in vitro. Hum Mol Genet 13:727-739.
19. Amit M, MK Carpenter, MS Inokuma, CP Chiu, CP Harris, MA Waknitz, J Itskovitz-Eldor and JA Thomson. (2000). Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227:271-278.
20. Bodnar MS, JJ Meneses, RT Rodriguez and MT Firpo. (2004). Propagation and maintenance of undifferentiated human embryonic stem cells. Stem Cells Dev 13:243-253.
21. Clark AT, RT Rodriguez, MS Bodnar, MJ Abeyta, MI Cedars, PJ Turek, MT Firpo and RA Reijo Pera. (2004). Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. Stem Cells 22:169-179.
22. Lois C, EJ Hong, S Pease, EJ Brown and D Baltimore. (2002). Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295:868-872.
23. Naldini L, U Blomer, P Gallay, D Ory, R Mulligan, FH Gage, IM Verma and D Trono. (1996). In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272:263-267.
24. Gaur M, GJ Murphy, J Frampton and AD Leavitt. (2004). Using retroviruses to express genes in primary megakaryocyte lineage cells. Methods Mol Biol 273:381-396.
25. Mitalipova MM, RR Rao, DM Hoyer, JA Johnson, LF Meisner, KL Jones, S Dalton and SL Slice. (2005). Preserving the genetic integrity of human embryonic stem cells. Nature Biotechnol 23:19-20.
26. Draper JS, K Smith, P Gokhale, HD Moore, E Maltby, J Johnson, L Meisner, TP Zwaka, JA Thomson and PW Andrews. (2004). Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nature Biotechnol 22:53-54.
27. Inzunza J, S Sahlen, K Holmberg, AM Stromberg, H Teerijoki, E Blennow, O Hovatta and H Malmgren. (2004). Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation. Mol Hum Reprod 10:461-466.
28. Gerrard L, D Zhao, AJ Clark and W Cui. (2005). Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Stem Cells 23:124-133.
29. Heins N, A Lindahl, U Karlsson, M Rehnstrom, G Caisander, K Emanuelsson, C Hanson, H Semb, P Bjorquist, P Sartipy and J Hyllner. (2006). Clonal derivation and characterization of human embryonic stem cell lines. J Biotechnol 122:511-520.
30. Sidhu KS and BE Tuch. (2006). Derivation of three clones from human embryonic stem cell lines by FACS sorting and their characterization. Stem Cells Dev 15:61-69.
31. Ventura A, A Meissner, CP Dillon, M McManus, PA Sharp, L Van Parijs, R Jaenisch and T Jacks. (2004). Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci USA 101:10380-10385.
32. Nolden L, F Edenhofer, S Haupt, P Koch, FT Wunderlich, H Siemen and O Brustle. (2006). Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase. Nature Methods 3:461-467.