Genetic and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongation

Journal of Experimental Botany

Volumn 58, Issue 3, 2007, Pages 473-481

  Qin, Yong Mei ^a   Pujol, François M ^b   Hu, Chun Yang ^a   Feng, Jian Xun ^a   Kastaniotis, Alexander J ^b   Hiltunen, J Kalervo ^b   Zhu, Yu Xian ^a  

^a PEKING UNIVERSITY   (China)

^b UNIVERSITY OF OULU   (Finland)

Author keywords

3 ketoacyl CoA synthase; Fatty acid elongation; Gossypium hirsutum; MALDI TOF MS

Indexed keywords

3-KETOACYL-COA SYNTHASE; FATTY ACID ELONGATION; GOSSYPIUM HIRSUTUM; MALDI-TOF-MS;

COTTON FIBERS; FATTY ACIDS; GENES;

YEAST;

ACYLTRANSFERASE; ARABIDOPSIS PROTEIN; CUT1 PROTEIN, ARABIDOPSIS; FATTY ACID; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COTTON; ENZYMOLOGY; GENETIC COMPLEMENTATION; GENETIC TRANSFORMATION; GENETICS; IN SITU HYBRIDIZATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; TRANSGENIC ORGANISM;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; ARABIDOPSIS PROTEINS; FATTY ACIDS; GENETIC COMPLEMENTATION TEST; GOSSYPIUM; IN SITU HYBRIDIZATION; MOLECULAR SEQUENCE DATA; ORGANISMS, GENETICALLY MODIFIED; PLANT PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSFORMATION, GENETIC;

COTTON; FATTY ACIDS; GENES; YEASTS;

ARABIDOPSIS; GOSSYPIUM HIRSUTUM; SACCHAROMYCES CEREVISIAE;

EID: 33847646792     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erl218     Document Type: Article

References (36)

1. Ayorinde FO, Garvin K, Saeed K. 2000. Determination of the fatty acid composition of saponified vegetable oils using matrixassisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 14, 608-615.
2. Dunn TM, Lynch DV, Michaelson LV, Napier JA. 2004. A postgenomic approach to understanding sphingolipid metabolism in Arabidopsis thaliana. Annals of Botany 93, 483-497.
3. Feng J-X, Ji S-J, Shi Y-H, Xu Y, Wei G, Zhu Y-X. 2004. Analysis of five differentially expressed gene families in fast elongating cotton fiber. Acta Biochimica et Biophysica Sinica 36, 51-56.
4. Fiebig A, Mayfield JA, Miley NL, Chau S, Fischer RL, Preuss D. 2000. Alterations in CER6, a gene identical to CUT1, differentially affect long-chain lipid content on the surface of pollen and stems. The Plant Cell 12, 2001-2008.
5. Han J, Luhs W, Sonntag K, Zahringer U, Borchardt DS, Wolter FP, Heinz E, Frentzen M. 2001. Functional characterization of β-ketoacyl-CoA synthase genes from Brassica napus L. Plant Molecular Biology 46, 229-239.
6. Hooker TS, Millar AA, Kunst L. 2002. Significance of the expression of the CER6 condensing enzyme for cuticular wax production in arabidopsis. Plant Physiology 129, 1568-1580.
7. Ghanevati M, Jaworski JG. 2002. Engineering and mechanistic studies of the Arabidopsis FAE1 β-ketoacyl-CoA synthase, FAE1 KCS. European Journal of Biochemistry 269, 3531-3539.
8. Gong W, Shen Y-P, Ma L-G, et al. 2004. Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Plant Physiology 135, 773-782.
9. James Jr DW, Lim E, Keller J, Plooy I, Ralston E, Dooner HK. 1995. Directed tagging of the Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene with the maize transposon activator. The Plant Cell 7, 309-319.
10. Ji S-J, Lu Y-C, Feng J-X, Wei G, Li J, Shi Y-H, Fu Q, Liu D, Luo J-C, Zhu Y-X. 2003. Isolation and analyses of genes preferentially expressed during early cotton fiber development by substractive PCR and cDNA array. Nucleic Acids Research 31, 2534-2543.
11. Ji S-J, Lu Y-C, Li J, Wei G, Liang X, Zhu Y-X. 2002. A β-tubulin-like cDNA expressed specifically in elongating cotton fibers induces longitudinal growth of fission yeast. Biochemical and Biophysical Research Communications 296, 1245-1250.
12. Kajikawa M, Yamato KT, Kanamaru H, Sakuradani E, Shimizu S, Fukuzawa H, Sakai Y, Ohyama K. 2003. MpFAE3, a β-ketoacyl-CoA synthase gene in the liverwort Marchantia polymorpha L., is preferentially involved in elongation of palmitic acid to stearic acid. Bioscience Biotechnology Biochemistry 67, 1667-1674.
13. Kastaniotis AJ, Autio KJ, Sormunen RT, Hiltunen JK. 2004. Htd2p/Yhr067p is a yeast 3-hydroxyacyl-ACP dehydratase essential for mitochondrial function and morphology. Molecular Microbiology 53, 1407-1421.
14. Kunst L, Samuels AL. 2003. Biosynthesis and secretion of plant cuticular wax. Progress in Lipid Research 42, 51-80.
15. Lassner MW, Lardizabal K, Metz JG. 1996. A jojoba β-ketoacyl-CoA synthase cDNA complements the canola fatty acid elongation mutation in transgenic plants. The Plant Cell 8, 281-292.
16. Lu YC. 2002. Large scale cloning and characterization of fiberspecific genes through high throughput analysis. PhD thesis, Peking University, China.
17. Ma DP, Tan H, Si Y, Creech RG, Jenkins JN. 1995. Differential expression of a lipid transfer protein gene in cotton fiber. Biochimica et Biophysica Acta 1257, 81-84.
18. Matthes B, Böger P. 2002. Chloroacetamides affect the plasma membrane. Zeitschrift Naturforschung [C] 57, 843-852.
19. Mietkiewska E, Giblin EM, Wang S, Barton DL, Dirpaul J, Brost JM, Katavic V, Taylor DC. 2004. Seed-specific heterologous expression of a Nasturtium FAE gene in Arabidopsis results in a dramatic increase in the proportion of erucic acid. Plant Physiology 136, 2665-2675.
20. Millar AA, Clemens S, Zachgo S, Giblin EM, Taylor DC, Kunst L. 1999. CUT1, an Arabidopsis gene required for cuticular wax biosynthesis and pollen fertility, encodes a very-long-chain fatty acid condensing enzyme. The Plant Cell 11, 825-838.
21. Millar AA, Kunst L. 1997. Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. The Plant Journal 12, 121-131.
22. Moon H, Chowrira G, Rowland O, Blacklock BJ, Smith MA, Kunst L. 2004. A root-specific condensing enzyme from Lesquerella fendleri that elongates very-long-chain saturated fatty acids. Plant Molecular Biology 56, 917-927.
23. Oh C-S, Toke DA, Mandala S, Martin CE. 1997. ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. Journal of Biological Chemistry 272, 17376-17384.
24. Paul S, Gable K, Beaudoin F, Cahoon E, Jaworski J, Napier JA, Dunn TM. 2006. Members of the Arabidopsis FAE1-like. 3-ketoacyl-CoA synthase gene family substitute for the Elop proteins of Saccharomyces cerevisiae. Journal of Biological Chemistry 281, 9018-9029.
25. Qin Y-M, Pujol FMA, Shi Y-H, Feng J-X, Liu Y-M, Kastaniotis AJ, Hiltunen JK, Zhu Y-X. 2005. Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductases from developing cotton fibers. Cell Research 15, 465-473.
26. Ruan Y-L, Chourey PS. 1998. A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds. Plant Physiology 118, 399-406.
27. Shi YH, Zhu SW, Mao XZ, Feng JX, Qin YM, Zhang L, Cheng J, Wei LP, Wang ZY, Zhu YX. 2006. Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. The Plant Cell 18, 651-664.
28. Schreiber L, Skrabs M, Hartmann K, Becker D, Cassagne C, Lessire R. 2000. Biochemical and molecular characterization of corn (Zea mays L.) root elongases. Biochemical Society Transactions 28, 647-649.
29. Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.
30. Todd J, Post-Beittenmiller D, Jaworski JG. 1999. KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana. The Plant Journal 17, 119-130.
31. Toke DA, Martin CE. 1996. Isolation and characterization of a gene affecting fatty acid elongation in Saccharomyces cerevisiae. Journal of Biological Chemistry 271, 18413-18422.
32. Trenkamp S, Martin W, Tietjen K. 2004. Specific and differential inhibition of very-long-chain fatty acid elongases from Arabidopsis thaliana by different herbicides. Proceedings of the National Academy of Sciences, USA 101, 11903-11908.
33. Tzagoloff A, Akai A, Foury F. 1976. Assembly of the mitochondrial membrane system. XVI. Modified form of the ATPase proteolipid in oligomycin-resistant mutants of Saccharomyces cerevisiae. FEBS Letters 65, 391-395.
34. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. 1997. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22, 130-131, 134-138.
35. Zhang T, Pan J. 1992. Genetic analysis of a fuzzless-lintless mutant in Gossypium hisutum L. Jiangsu Journal of Agriculture Science 7, 13-16.
36. Zheng H, Rowland O, Kunst L. 2005. Disruptions of the Arabidopsis enoyl-CoA reductase gene reveal an essential role for very-long-chain fatty acid synthesis in cell expansion during plant morphogenesis. The Plant Cell 17, 1467-1481.