Noninvasive image analysis of 3D construct mineralization in a perfusion bioreactor

Biomaterials

Volumn 28, Issue 15, 2007, Pages 2525-2533

  Porter, Blaise D ^a   Lin, Angela S P ^a,b   Peister, Alexandra ^b   Hutmacher, Dietmar ^c   Guldberg, Robert E ^a,b  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Bioreactor; Bone tissue engineering; Image analysis

Indexed keywords

CELLS; DEPOSITION; IMAGE ANALYSIS; MINERALS; OPTIMIZATION; PARTICLE SIZE;

MINERAL DEPOSITION; MINERALIZATION;

BIOREACTORS;

MINERAL;

ANIMAL CELL; ANIMAL TISSUE; ARCHITECTURE; ARTICLE; BIOREACTOR; CELL TYPE; IMAGE ANALYSIS; MICRO-COMPUTED TOMOGRAPHY; MINERALIZATION; MONITORING; NON INVASIVE PROCEDURE; NONHUMAN; PARTICLE SIZE; PERFUSION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; THICKNESS; THREE DIMENSIONAL IMAGING;

ANIMALS; BIOCOMPATIBLE MATERIALS; BIOREACTORS; BONE AND BONES; CALCIFICATION, PHYSIOLOGIC; COLLAGEN; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, CONFOCAL; PERFUSION; POLYESTERS; RATS; RATS, SPRAGUE-DAWLEY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; STROMAL CELLS; TISSUE ENGINEERING; TOMOGRAPHY, X-RAY COMPUTED;

EID: 33847644129     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2007.01.013     Document Type: Article

References (27)

1. Schantz J.T., Hutmacher D.W., Lam C.X., Brinkmann M., Wong K.M., Lim T.C., et al. Repair of calvarial defects with customised tissue-engineered bone grafts II. Evaluation of cellular efficiency and efficacy in vivo. Tissue Eng 9 Suppl. 1 (2003) S127-S139
2. Bruder S.P., and Fox B.S. Tissue engineering of bone. Cell based strategies. Clin Orthop Relat Res Suppl. 367 (1999) S68-S83
3. Lane J.M., Yasko A.W., Tomin E., Cole B.J., Waller S., Browne M., et al. Bone marrow and recombinant human bone morphogenetic protein-2 in osseous repair. Clin Orthop Relat Res 361 (1999) 216-227
4. Einhorn T.A. Clinically applied models of bone regeneration in tissue engineering research. Clin Orthop Suppl. 367 (1999) S59-S67
5. Brodke D., Pedrozo H.A., Kapur T.A., Attawia M., Kraus K.H., Holy C.E., et al. Bone grafts prepared with selective cell retention technology heal canine segmental defects as effectively as autograft. J Orthop Res 24 5 (2006) 857-866
6. Byers B.A., Guldberg R.E., and Garcia A.J. Synergy between genetic and tissue engineering: Runx2 overexpression and in vitro construct development enhance in vivo mineralization. Tissue Eng 10 11-12 (2004) 1757-1766
7. Ishaug-Riley S.L., Crane-Kruger G.M., Yaszemski M.J., and Mikos A.G. Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers. Biomaterials 19 15 (1998) 1405-1412
8. Cartmell S., Huynh K., Lin A., Nagaraja S., and Guldberg R. Quantitative microcomputed tomography analysis of mineralization within three-dimensional scaffolds in vitro. J Biomed Mater Res 69A 1 (2004) 97-104
9. Bancroft G.N., Sikavitsas V.I., van den Dolder J., Sheffield T.L., Ambrose C.G., Jansen J.A., et al. Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner. Proc Natl Acad Sci USA 99 20 (2002) 12600-12605
10. Cartmell S.H., Porter B.D., Garcia A.J., and Guldberg R.E. Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro. Tissue Eng 9 6 (2003) 1197-1203
11. Glowacki J., Mizuno S., and Greenberger J.S. Perfusion enhances functions of bone marrow stromal cells in three-dimensional culture. Cell Transplant 7 3 (1998) 319-326
12. Goldstein A.S., Juarez T.M., Helmke C.D., Gustin M.C., and Mikos A.G. Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds. Biomaterials 22 11 (2001) 1279-1288
13. Holtorf H.L., Jansen J.A., and Mikos A.G. Flow perfusion culture induces the osteoblastic differentiation of marrow stroma cell-scaffold constructs in the absence of dexamethasone. J Biomed Mater Res A 72 3 (2005) 326-334
14. Janssen F.W., Oostra J., Oorschot A., and van Blitterswijk C.A. A perfusion bioreactor system capable of producing clinically relevant volumes of tissue-engineered bone: in vivo bone formation showing proof of concept. Biomaterials 27 3 (2006) 315-323
15. Meinel L., Karageorgiou V., Fajardo R., Snyder B., Shinde-Patil V., Zichner L., et al. Bone tissue engineering using human mesenchymal stem cells: effects of scaffold material and medium flow. Ann Biomed Eng 32 1 (2004) 112-122
16. Braccini A., Wendt D., Jaquiery C., Jakob M., Heberer M., Kenins L., et al. Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts. Stem Cells 23 8 (2005) 1066-1072
17. Sikavitsas V.I., Bancroft G.N., Holtorf H.L., Jansen J.A., and Mikos A.G. Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces. Proc Natl Acad Sci USA 100 25 (2003) 14683-14688
18. Klein-Nulend J., Helfrich M.H., Sterck J.G., MacPherson H., Joldersma M., Ralston S.H., et al. Nitric oxide response to shear stress by human bone cell cultures is endothelial nitric oxide synthase dependent. Biochem Biophys Res Commun 250 1 (1998) 108-114
19. McAllister T.N., Du T., and Frangos J.A. Fluid shear stress stimulates prostaglandin and nitric oxide release in bone marrow-derived preosteoclast-like cells. Biochem Biophys Res Commun 270 2 (2000) 643-648
20. Porter B., Zauel R., Stockman H., Guldberg R., and Fyhrie D. 3-D computational modeling of media flow through scaffolds in a perfusion bioreactor. J Biomech 38 3 (2005) 543-549
21. Schantz J.T., Lim T.C., Ning C., Teoh S.H., Tan K.C., Wang S.C., et al. Cranioplasty after trephination using a novel biodegradable burr hole cover: technical case report. Neurosurgery 58 Suppl. 1 (2006) ONS-E176; discussion ONS-E176
22. Aubin J.E. Osteoprogenitor cell frequency in rat bone marrow stromal populations: role for heterotypic cell-cell interactions in osteoblast differentiation. J Cell Biochem 72 3 (1999) 396-410
23. Hutmacher D.W., Schantz T., Zein I., Ng K.W., Teoh S.H., and Tan K.C. Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. J Biomed Mater Res 55 2 (2001) 203-216
24. Byers B.A., and Garcia A.J. Exogenous Runx2 expression enhances in vitro osteoblastic differentiation and mineralization in primary bone marrow stromal cells. Tissue Eng 10 11-12 (2004) 1623-1632
25. Bonewald L.F., Harris S.E., Rosser J., Dallas M.R., Dallas S.L., Camacho N.P., et al. von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation. Calcif Tissue Int 72 5 (2003) 537-547
26. Martin I., Wendt D., and Heberer M. The role of bioreactors in tissue engineering. Trends Biotechnol 22 2 (2004) 80-86
27. Ishaug S.L., Crane G.M., Miller M.J., Yasko A.W., Yaszemski M.J., and Mikos A.G. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. J Biomed Mater Res 36 1 (1997) 17-28