Screening and characterization of a novel fibrinolytic metalloprotease from a metagenomic library

Biotechnology Letters

Volumn 29, Issue 3, 2007, Pages 465-472

  Lee, Dong Geun ^a   Jeon, Jeong Ho ^b   Jang, Min Kyung ^a   Kim, Nam Young ^a   Lee, Jong Hyun ^a   Lee, Jung Hyun ^b   Kim, Sang Jin ^b   Kim, Gun Do ^c   Lee, Sang Hyeon ^a  

^a Silla University   (South Korea)

^b Korea Research Institute of Ships and Ocean Engineering   (South Korea)

^c Pukyong National University   (South Korea)

Author keywords

Azocasein; Fibrinolytic; Metagenome; Metalloprotease

Indexed keywords

AMINO ACIDS; DNA; ESCHERICHIA COLI; GENES; HYDROLYSIS; PH EFFECTS;

AZOCASEIN; FIBRINOLYTIC; METAGENOME; METALLOPROTEASE;

ENZYMES;

BACTERIAL PROTEIN; FIBRINOLYTIC AGENT; METALLOPROTEINASE; PEPTIDE LIBRARY; PROTEOME;

ARTICLE; CHEMISTRY; CHROMOSOME MAP; DRUG DESIGN; ESCHERICHIA COLI; GENETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; SEDIMENT;

BACTERIAL PROTEINS; CHROMOSOME MAPPING; DRUG DESIGN; ESCHERICHIA COLI; FIBRINOLYTIC AGENTS; GEOLOGIC SEDIMENTS; METALLOPROTEASES; PEPTIDE LIBRARY; PROTEOME;

ESCHERICHIA COLI;

EID: 33847263157     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9263-8     Document Type: Article

References (28)

1. Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143-169
2. Bode W, Gomis-Rüth F-X, Stöckler W (1993) Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc-binding environments (HEXXHXXGXXH and Met-turn) and topologies and should be grouped into a common family, the 'metzincins'. FEBS Lett 331:134-140
3. Datta G, Dong A, Witt J, Tu AT (1995) Biochemical-characterization of basilase, a fibrinolytic protease from Crotalus basiliscus basiliscus. Arch Biochem Biophys 317:365-373
4. Ferrer M, Martinez-Abarca F, Golyshin PN (2005) Mining genomes and 'metagenomes' for novel catalysts. Curr Opin Biotechnol 16:588-593
5. Fujita M, Nomura K, Hong K, Ito Y, Asada A, Nishimuro S (1993) Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochem Biophys Res Commun 197:1340-1347
6. Gupta R, Beg QK, Lorenz P (2002) Bacterial alkaline protease: molecular approaches and industrial application. Appl Microbial Biotechnol 59:15-32
7. Holden RW (1990) Plasminogen activators: pharmacology and therapy. Radiology 174:993-1001
8. Hurt RA, Qiu X, Wu L, Roh Y, Palumbo AV, Tiedje JM, Zhou J (2001) Simultaneous recovery of RNA and DNA from soils and sediments. Appl Environ Microbiol 67:4495-4503
9. Jeong YK, Yang WS, Kim KH, Chung KT, Joo WH, Kim JH, Kim DE, Park JU (2004) Purification of a fibrinolytic enzyme (myulchikinase) from pickled anchovy and its cytotoxicity to the tumor cell lines. Biotechnol Lett 26:393-397
10. Kim JH, Kim YS (2001) Characterization of a metalloenzyme from a wild mushroom, Tricholoma saponaceum. Biosci Biotechnol Biochem 65:356-362
11. Kim OS, Kwak HJ, Lee J-H, Ha JM, Ha B-J, Lee S-H (2004) Development of an E.coli expression cassette for the efficient production of a harmful protein. Biotechnol Bioproc Eng 9:389-392
12. Larsen KS, Auld DS (1991) Characterization of an inhibitory metal binding site in carboxypeptidase A. Biochemistry 30:2613-2618
13. Lee SH, Oh JR, Lee JH, Kim SJ, Cho JC (2004) Cold-seep sediment harbors phylogenetically diverse uncultured bacteria. J Microbiol Biotechnol 14:906-913
14. Lee SH, Taguchi H, Yoshimura E, Minagawa E, Kaminogawa S, Ohta T, Matsuzawa H (1994) Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli. Biosci Biotechnol Biochem 58:1490-1495
15. Leonardi A, Gubensek F, Krizaj I (2002) Purification and characterisation of two hemorrhagic metalloproteinases from the venom of the long-nosed viper, Vipera ammodytes ammodytes. Toxicon 40:55-62
16. Luciano P, Tokatlidis K, Chambre I, Germanique JC, Geli V (1998) The mitochondrial processing peptidase behaves as a zinc-metallopeptidase. J Mol Biol 280:193-199
17. Marchler-Bauer A, Anderson JB, Cherukuri PF, DeWeese-Scott C, Geer LY, Gwadz M, He S, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Liebert CA, Liu C, Lu F, Marchler GH, Mullokandov M, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Yamashita RA, Yin JJ, Zhang D, Bryant SH (2005) CDD: a Conserved Domain Database for protein classification. Nucleic Acids Res 33:D192-196
18. Nakajima N, Mihara H, Sumi H (1993) Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus. Biosci Biotechnol Biochem 57:1726-1730
19. Poindexter K, Nelson N, DuBose RF, Black RA, Cerretti DP (1999) The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries. Gene 237:61-70
20. Ramos OH, Carmona AK, Selistre-de-Araujo HS (2003) Expression, refolding, and in vitro activation of a recombinant snake venom pro-metalloprotease. Protein Expr Purif 28:34-41
21. Ratel D, Glazier G, Provencal M, Boivin D, Beaulieu E, Gingras D, Beliveau R (2005) Direct-acting fibrinolytic enzymes in shark cartilage extract: potential therapeutic role in vascular disorders. Thromb Res 115:143-152
22. Sambrook E, Fritsch F, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Labarotory, Cold Spring Harbor, New York
23. Sharma S, Aneja MK, Mayer J, Schloter M, Munch JC (2004) RNA fingerprinting of microbial community in the rhizosphere soil of grain legumes. FEMS Microbiol Lett 240:181-186
24. Strohl WR (2000) The role of natural products in modern drug discovery. Drug Discov Today 5:39-41
25. Sumi H, Nakajima N, Yatagai C (1995) A unique strong fibrinolytic enzyme (katsuwokinase) in skipjack "Shiokara," a Japanese traditional fermented food. Comp Biochem Physiol B Biochem Mol Biol 112:543-547
26. Vallee BL, Auld DS (1990) Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry 29:5647-5659
27. Windle HJP, Kelleher D (1997) Identification and characterization of a metalloprotease activity from Helicobacter pylori. Infect Immun 65:3132-3137
28. Wong AH, Mine Y (2004) Novel fibrinolytic enzyme in fermented shrimp paste, a traditional asian fermented seasoning. J Agric Food Chem 52:980-986